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We narrowed to 653 results for: EPO

Showing: 301 - 310 of 653 results

  1. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...sensors are better classified by their fluorescent reporter and the mechanism of their voltage sensing component...genetic cargo in lieu of their own genomes and deposit this cargo upon host infection. Packaging viruses...

  2. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ... nuclease active site wasn’t even close to its reported cleavage site on the complementary DNA strand....model also displays the several Lys/Ala mutations reported by Ian Slaymaker and colleagues to reduce off-...

  3. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...sections of DNA can be more difficult. Addgene depositors have developed new CRISPR-based methods for various...or cell lines, please consider sharing them by depositing with Addgene! Figures were adapted from: Hall...

  4. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...easier to construct targeting vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed MMEJ to create...Pages Find CRISPR Plasmids for Your Experiments Deposit Your New Plasmid Tools with Us  ...

  5. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...experimental settings. It is a curated set of technical reports on AAV vectors from Addgene and the CLOVER Center...Center, each one submitted by researchers like you. Reports are reviewed by Addgene scientists for clarity ...

  6. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...diseases and metabolic diseases are also underway and reported results are encouraging. Importantly, a significant...combined with AAV for in vivo gene editing, and reports suggest that the first clinical study combining...

  7. An Introduction to Adenovirus

    Type
    Blog Post
    ...(Tollefson et al., 1996) – cell death has been reported when using vectors, and immune inactivation of...pre-existing toolbox for adenoviral vectors in Addgene’s repository is smaller than for other vector types, we hope...

  8. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...viral vector system that could do both, Addgene depositor Didier Trono and collaborators turned to the lentivirus...transduced rat neurons in vivo with beta-gal and other reporters, and they showed that expression persisted for...

  9. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...in mammalian cells, LbCpf1 and AsCpf1. In both reports, on target editing efficiency for the Cpf1 orthologs...mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions...

  10. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...even been linked to some diseases. It has been reported that 5-10% of human genes contain a region where...EMBO Rep 1:18–23 . https://doi.org/10.1093/embo-reports/kvd001 Additional resources on the Addgene blog...
Showing: 301 - 310 of 653 results