We narrowed to 1,073 results for: TOR;

... College of Medicine. Her doctoral research was conducted in the laboratory of Erik Lee Snapp and focused...the cytoplasm. That is, unfortunately, some investigators realize too late that they have missed the impact...environment where it will localize [4]. Most investigators are surprised to learn that FPs are often poorly...environments, including the organelles that compose the secretory pathway, endocytic vesicles, mitochondria, lysosomes...residues form inappropriate disulfide bonds in the secretory pathway (Figure 1) [4,6–8]. Additionally, FPs ...and a sequence intended to localize them to the secretory pathway, a dark pool that is mislocalized to the...misfolded, blocked from proceeding through the secretory pathway, and retained in the ER. FPs have been...

...therapeutics such as  (Chimeric Antigen Receptors) CAR T cells and PD-1 modulators. An exciting development in immunotherapy...methodology with T cell receptor engineering (Ref 5)  as described by Blue Flame Addgene depositor Professor Michel...Professor Karin about alcohol and obesity as driving factors for chronic inflammation which in turn suppresses...self-limiting effects kick in trying to resolve the inflammatory state by inducing immunosuppressive measures...upregulation of the immunosuppressive cell surface receptor PD-1. This immunosuppressive environment in turn...tumor and immune cells in real time. Addgene depositor Professor Max Krummel from UCSF showed some impressive...Throughout the conference aspirin and the use of COX inhibitors was discussed and Professor Caetano Reis e Sousa...

...then easy to package in low-capacity vectors like AAV, a popular vector due to its low immunogenicity. But...RNA-editing Cas13 enzymes have taken the CRISPR world by storm. Like RNA interference, these enzymes can knock ...in size and are easy to package in low-capacity vectors! These small, but mighty type VI-D enzymes are ...looked for a CRISPR repeat array near a putative effector nuclease. The Cas13d proteins they identified ...identified other advantages, including Cas13d-specific regulatory proteins and high targeting efficiency, both ...WYL domain-containing proteins may be broader regulators of Cas13d activity. This property makes WYL proteins...Konermann et al.: CasRx is a highly potent, specific editor in mammalian cells Not all Cas13d proteins are ...

...  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David Root lab is...you! We would like to thank all of our depositors and requestors for making their reagents available and...opening our UK office in 2014, to starting our viral vector service in 2016...we’re excited to help scientists...look back at some of the contributions from our depositors. Below are the 15 top requested plasmids over... be used with 2nd or 3rd generation lentiviral vectors and an envelope expressing plasmid, such as our...lentiCRISPR v2 - This 3rd generation lentiviral transfer vector also from the Zhang lab is used to deliver Cas9...your gRNA of interest into mammalian cells. This vector was the top requested plasmid of 2015. What makes...

...adenine base editor Earlier this year, Keith Joung’s lab developed SECURE cytosine base editors, which decrease... Nature Biotechnology Find the base editors   Single base editor that induces C-to-T and A-to-G mutations...developed a base editor that combines the abilities of C-to-T and A-to-G base editors into one protein...pyogenes Cas9. They called this editor Target-ACE (adenine and cytosine editor). Target-ACE exhibits increased...research! This time: CRISPRi in Burkholderia Base editors CRISPR toolkit for epitope tagging Lentiviral capsid-based...APOBEC1. Now, they’ve engineered SECURE adenine base editor variants using a protein-truncation strategy with...a target site compared to a mixture of two base editor enzymes in mammalian cells. Read the paper in ...

...activation of keratinocytes, which release inflammatory mediators that trigger the activation of dendritic...recruitment of neutrophils. Though there are IL-36 receptor inhibitors in development, there’s still a lot of mysteries...Generalized pustular psoriasis is a chronic autoinflammatory skin disease estimated to affect 0.27 to 4.6...actually get out of keratinocytes and induce their effector functions. It’s an important process that we need...IL-36 cytokines are a potent inducer of an autoinflammatory loop: keratinocytes both respond to and release...when he was studying thoracic aortic disease. “My mentors Dr. Dianna Milewicz, Dr. Carolyn Bondy, and Dr....with TS (Trolle et al., 2016). Using lentiviral vectors from Addgene, the lab will express these candidate...

...point.   What makes a story? A story is a narrative connecting cause and effect. Stories can and should be... or notes. Listen to their stories and narratives (see below “Tell a story”) and note how they build suspense...hear material delivered in the form of a story. Using stories is one of the best ways to make you and ...me and wished I had been more careful. 3. Tell a story We are biologically programmed to listen and remember...presentations engaging and memorable. If you read my story above about the presentation faux pas, you are more... striking images, creative ideas, and engaging stories as you encounter or experience them. Drop content... all graduate students and postdocs form peer mentoring groups to practice giving talks and getting feedback...

...editing assemblies are sensor-actuators, either transcriptional regulators or chromatin-modifying enzymes...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...s chromatin status into account (Fig 1a). The laboratory of Karmella Haynes at Emory recently contributed...contributed a set of plasmids to the repository that will help epigenetic researchers custom design their own...Examples of histone-modifying enzymes and reader-effector proteins. b) Golden Gate assembly (backbones available...with examples of potential synthetic chromatin mediators and probes. Image from Priode et al., 2021. ...potential tools fall into two categories: probes and editors. A probe array may consist of at least two sensor...

...lab has developed AAV vectors to selectively visualize excitatory and inhibitory synapses in the brain...combining vectors expressing different fluorescent proteins targeting inhibitory or excitatory synapses... find in this post: Tools for labeling excitatory inhibitory synaptic proteins Fluorescent protein system...New from the viral service Labeling excitatory and inhibitory synaptic proteins with FingRs By Jason... proteins PSD95 and gephyrin to label excitatory and inhibitory synapses, respectively. Once expressed...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles... cycle in vivo, particularly if the cells are migratory or in deep tissues. To resolve this issue, Shangqin...

...efficiencies of integrating viral vectors. New Sleeping Beauty transposase vectors Even more recently, Eric ...developed two new types of vectors for the SB transposase system - one set of vectors for robust constitutive...transposase as a nonviral vector for CD19-directed chimeric antigen receptor (CAR) therapy. SB is a valuable...of DNA that can move around in a genome. In a laboratory setting, transposons can be used to both introduce...cells’ defense mechanisms. The use of non-viral vectors, like transposons, avoids many, though not all,...use of viruses is also important for social and regulatory reasons. 1.) The transposon consists of a mirrored... expression (pSBbi derivatives) and one set of vectors for inducible expression (pSBtet derivatives). ...