... et al. designed gRNAs to target the HIV-1 5’ and 3’ LTRs and expressed them along with Cas9 in the T-...eradicating HIV-1/AIDS. J Neurovirol. 2015 Jun;21(3):310-21. PubMed PMID: 25716921. Additional Resources...reservoirs, chronic inflammation, and negative metabolic effects of ART. Why can’t the body mount an immune...

...Blog (other programs are discussed below). Chpater 3: SITN Popularity and Events - Amy discusses some of...To learn more about the STIN's podcast, email [email protected]    For more info about starting your...evens where scientists can grab a beer and chat about their work at a local bar. It doesn't have to be...1: Introduction - Vini and Amy tell us a little about themselves. Chapter 2: What is Science in the News...science communicators. In this chapter we learn about some of their events - science seminars, science...the lab and working with SITN. She points to collaboration and delegating as keys achieving success. Chapter...your own SciComm organization and to learn about some of SITN's other initiatives, email [email protected]...

...for making scientific graphics. PS: they’re free. 3. Fluorescent Proteins 101: GFP Fusion Proteins - Making...mNeonGreen, mTFP1, and mWasabi by Jennifer Tsang Learn about mNeonGreen, mTFP1, and mWasabi and find plasmids...Broccoli, Spinach, and Corn, for example. Learn more about the aptamer soup in this blog post. 5. Five Popular...

...similarly but not identically to BE3, modifying a 3-5 base window 18 bases upstream of the PAM.  David...than BE2 Nishida et al., 2016 Target-AID   Edits 3-5 base window surrounding -18 position upstream of... efficiency and product purity. Science Advances, 3(8), eaao4774. https://doi.org/10.1126/sciadv.aao4774...Homology-Directed Repair Learn about base editing for plants Read about four base editor reporters Additional... The BE3 system increased editing frequency to above 30% for a variety of targets in human cell lines... Liu lab created BE4max and AncBE4max mentioned above, they also made an adenine base editor with improved...cell therapy applications. Dual base editors What about combining the function of both editors into one?...

...the human kinome. These plasmids contain the same 3-4 gRNAs found in the first subset of the pooled library...numbers that’ll leave you, well, #sad. Learn More About CRISPR Pooled Library Screening Why the kinome?...

... bar to quickly find what you’re looking for. Tip 3: Share protocols and spreadsheets with lab members...experiments before the next R01 is due. But what about organizing yourself and your lab? Organizing your...

...tissue-penetrating wavelength above 600 nm. LumiFluors Schaub, F.X., et al. (3) designed two imaging probes...PMID: 27465542. PubMed Central PMCID: PMC4974478. 3. Schaub, F.X., et al. (2015) Fluorophore-NanoLuc BRET...Blog Learn more about FRET Tips for Using FRET in Your Experiments Learn more about Fluorescent Protein...heme-containing proteins in whole animals, light at or above 600 nm wavelengths should be used. Researchers often...be excited by NanoLuc® luciferase (NLuc) and set about to create an in vivo imaging reporter by linking...

...For treatment, it is best to identify a cocktail of 3-12 phages. Once these phages are identified, they ...open science Find blog posts about scientific sharing Find blog posts about networking Additional resources...

... Transcriptional repression DNA Methyltransferase 3 Alpha (DNMT3A) Vlatka Zoldoš’ lab has deposited pdCas9...available as ready-to-use lentivirus. Figure 3: dCas9-DNMT3A methylates cytosines at promoters and...modifications. Like the TALE-LSD1 system described above, dCas9-LSD1 inactivates targeted enhancers. Although...setting up your experimental system. Let us know about your experience with these constructs in the comments...

... leaving behind any accessory DNA sequences [2], [3]. Simply put, the piggyBac™ transposon containing ...PMID: 26702451. PubMed Central PMCID: PMC4686154. 3. A. M. Singh, D. W. Perry, V. V. A. Steffey, K. Miller...transposase itself, please read on to learn more about this exciting technology from the folks at Transposagen...Additional Resources on the Addgene Blog Learn about the Sleeping Beauty Transposase Read up on Genome...