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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 818 results for: cell

Showing: 301 - 310 of 818 results

  1. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...with enrichment, single-cell sequencing, and optical pooled screens in human cells. Addgene offers vectors...reverse transcriptase enzymes, inhibition of host cell proteins, and modifications to the pegRNA. The Britt...shows enhanced editing efficiencies in a number of cell types at different genomic loci — in some cases,... editing efficiencies at different loci in U2OS cells. Image adapted from Yan et al. 2024 under a CC-BY...western blots (Figure 3).      Figure 3: HeLa cells transduced with SARS-CoV-2 nucleocapsid protein ...desirable than having many closely-packed labeled cells. Plus, Voltron2’s fluorescence is tunable by changing...Neuhaus Genetic modification of microglial immune cells is critical for central nervous system research;...

  2. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...differentiate induced pluripotent stem cells (Chavez et al., 2015) into neuronal cells by activating genes that cause...drug targets by finding genes that cause cell death in cancer cell lines upon activation (Gilbert et al.,...Repression and Activation. Cell 159:647–661 . https://doi.org/10.1016/j.cell.2014.09.029 Konermann S, Brigham...and Fluorescence Imaging. Cell 159:635–646 . https://doi.org/10.1016/j.cell.2014.09.039 Wong N, Liu W,... that over-express coding sequences from a given cell type or organism, have been used in a similar manner...many times, the isoform(s) common to a particular cell type are unknown or not readily available. By activating... et al., 2015)) are good choices across multiple cell lines (HEK293T, MCF7, U2-OS, Hela, N2A, 3T3) and...

  3. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    .... RANbodies are expressed in 293T cells and are secreted from cells due to their IgGκ signal peptide sequence...-to tool for detecting a protein of interest in cells and tissues. Although antibody production is well... sequence, making them easy to harvest from cell culture media. Their His tags allow for further purification...RANBody While all three reporters stain cultured cells and tissue sections, some reporters are better suited... signal amplification of HRP Staining cultured cells and tissue sections Amplifying weak signals of fluorescent...Labeled anti-HA or -Myc antibody Staining cultured cells and tissue sections When detected with a labeled...Labeled Anti-Chicken IgY antibody Staining cultured cells and tissue sections Great for multicolor staining...

  4. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System.” Cell 155 (2013): 1379-1491. Pubmed...of 6 chromosome-specific loci in the U20S cancer cell line./ Each color represents a gRNA targeting a ...localization and movement of this locus in live cells! This technique was developed in the lab of Addgene...et al. to track telomere dynamics throughout the cell cycle. To optimize the signal to noise ratio of ...sophisticated tracking of multiple chromosomal loci in live cells. However, they note that certain improvements could...repression and genome editing. Ready to make your cells a little more colorful? CRISPRainbow is available...Multicolor CRISPR labeling of chromosomal loci in human cells.” Proc. Natl. Acad. Sci. USA 112(10) (2015): 3002...

  5. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    ...sensitive GFP2 (roGFP2)-based biosensors for live cells. His work focuses on the enzyme hydropersulfide-... understand the processes that happen inside the cell,” says Pedre Pérez. Figure 2: Micrographs...Micrographs of the biosensor expressed in the human cell line, Hep3B. From left to right: Mitotracker Red, GFP...studies opioid use disorder. Her goal is to identify cell populations in the brain that contribute to the ...will explore the connectivity patterns of these cells and the functional contribution to a variety of ...administration and withdrawal to explore how these cells may encode the negative affect associated with drug...library of mutant sensors expressed in a mammalian cell line engineered to be electrically excitable. The...

  6. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ... defined population of cells (e.g., a neuron subtype) and specific subcellular compartments for measurements...calcium concentrations and transients in living cells and organisms. Unlike chemical indicators, these...neuron-specific expression (hSyn promoter) and pan-mammalian cell expression (CAG promoter). An improved version named...neuron-specific, astrocyte-specific (GCaMP6 only) or mammalian cell expression, and compatible with constitutive or ...measure changes in calcium specific to the neuronal cell body or the axon (GCaMP6 only). The genetic elements...long-term expression and integration within the target cells, ensuring more consistent and reproducible results...calcium sensor to specific tissues or even distinct cell subtypes for efficient expression and downstream...

  7. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...that inntags IT5, IT6, and IT10 expressed well in cells and did not form undesirable aggregates. Using budding...tags. Impressively, IT6-GFP did not reduce yeast cell size or viability compared to untagged GFP overexpression...multiple other proteins. Again, little to no change in cell morphology was observed with IT5 and IT6 fusions...and HA tags had especially detrimental effects on cell size and growth rate, indicating that they compromised...correlation for protein localization in mammalian cells." Nature methods 10.4 (2013): 315-323. Pubmed PMID...

  8. CRISPR Antimicrobials

    Type
    Blog Post
    ...reach essentially every cell or else small pools of antibiotic resistant cells could repopulate the environment...strategies are nonspecific - they harm any bacterial cell without a resistance gene, allowing resistant bacteria...antibiotics target one of three essential processes: cell wall synthesis, DNA replication, or protein synthesis...eliminated from the bacterium, which may also induce cell death. CRISPR also enables increased specificity...Treating a mixed population, represented here by cells of different colors, specifically targets bacteria...bacteria. Targeting a genomic sequence results in cell death. Plasmid targeting may result in plasmid loss...and antibiotic resensitization, or may also cause cell death. Image from Beisel et al., licensed under ...

  9. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ...choose cell lines that are highly transfectable.  The amounts of each plasmid transfected into cells are ... polymerase gene or using cells that stably express T7 (e.g. BSR T7/5 cells) (1). We hope that minigenomes...capable of being replicated and transcribed in the cell when the appropriate viral proteins are provided... RNA-dependent RNA polymerase into the infected cells. The EBOV polymerase replicates the viral genome...on separate plasmids and are co-transfected into cells alongside a plasmid encoding the minigenome. These...the minigenome) are efficiently transfected into cells. All of these components are necessary to efficiently... RNA polymerase also needs to be present in the cells to transcribe the viral mRNAs and minigenome. This...

  10. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...orthologs that display cleavage activity in mammalian cells. Like Cas9 nucleases, Cpf1 family members contain...the PAM. With this information, they turned to a cell culture system to see if any Cpf1 nucleases would...would exhibit in vivo activity in mammalian cells. From 16 diverse Cpf1 candidates, Zetsche et al. found...particularly helpful for targeting non-dividing cells, which are difficult to modify through homology-... Cpf1 orthologs known to be active in mammalian cells, LbCpf1 and AsCpf1. In both reports, on target editing... (RNPs) failed to induce off-target editing in a cell culture model. The application of Cpf1 to genome...RNA-guided endonuclease of a class 2 CRISPR-Cas system." Cell (2015). PubMed PMID: 26422227. Find plasmids from...
Showing: 301 - 310 of 818 results