We narrowed to 997 results for: lars;

...birds spots the “caterpillar” and then poke out the snail’s eyes and ingests the larvae. The adult worms...biosynthesis in Drosophila melanogaster." Molecular and cellular endocrinology 215.1-2 (2004): 1-10. PubMed...snail’s eyestalks. Once ingested by the snail, the larvae reach the eyestalk and transform them into a pulsating...pulsating display imitating a moving caterpillar. The worm controls the snail’s behavior, forcing it out...parasite The parasitic worm Spinochordodes tellinii‘s larvae makes its home inside grasshoppers and crickets...

...sensitivity in the zeptomolar range while One-step-SHERLOCK has a sensitivity in the femto-aptomolar range. For...fastest test that has been developed so far.  A similar method has been optimized to detect the SARS-CoV...molecule per milliliter can be detected – in the zeptomolar range Time: ~1 hour Equipment required: 37°C ...and will not give a positive result for highly similar viruses. Unlike Cas12a, Cas13a does not require...illuminator or UV light illuminator. Approach: Highly similar to the SHERLOCK diagnostic test, this test varies...identification using FnCas9 (FELUDA) Sensitivity: – 110 femtomolar  Time: Not mentioned, likely ~1-2 hours Equipment...Shravanti Suresh is a PhD candidate who investigates cellular aspects of CRISPR/Cas immune systems in the Sashital...

... what your desired edit is. Large knock-ins Are you introducing a large insertion, something 20 base ... applicable to knock-ins both large and small is a software very similar to TIDE known as TIDER (Tracking...deletion that isn’t a multiple of three will do. A popular screening method for knockouts is Tracking of Indels...edit. Most dual guide systems generate a deletion large enough that it can be visualized by running your...guide system.   Fig. 2: Size screening for large deletion events.   Validating knock-in mutations... Pro tip: make sure your PCR amplicon isn’t too large so that you can easily visualize the size shift ...

...should be widely applicable to other targets. The modular system of linkers and spacers could enable many...single-particle analysis of PRC2 with RvLEAMshort (1:6 molar ratio) with 10 minutes of glutaraldehyde crosslinking...previously-reported technique for precisely integrating large DNA sequences into a genome (Anzalone et al., 2022...deletion, replacement, integration and inversion of large DNA sequences with twin prime editing. Nature Biotechnology... (2024). Efficient site-specific integration of large genes in mammalian cells via continuously evolved...cells you are studying. Antibodies can help reveal cellular identities by targeting marker proteins that are...expressed in a given cell type. For example, glial fibrillary acidic protein (GFAP) is a glia-specific intermediate...

...Goedart, an assistant professor at the Section of Molecular Cytology and van Leeuwenhoek Centre for Advanced...Microscopy (University of Amsterdam). GFP is the most popular, most widely used genetically encoded fluorescent...fluorescent probe. Several factors contribute to the popularity of GFP including (i) fast and complete maturation...folded beta-barrel structure, followed by an intramolecular reaction that generates the chromophore (Tsien...Tsien, 1998). Importantly, this reaction requires molecular oxygen and as a consequence GFP remains non-fluorescent...Goedhart is an assistant professor at the Section of Molecular Cytology and Van Leeuwenhoek Centre for Advanced...Kurt. "Genetically encoded fluorescent tags." Molecular biology of the cell 28.7 (2017): 848-857. PubMed...

...dLight1, a molecular tool to detect and image DA in live animals (Patriarchi et al., 2018). Similar to calcium...the brain has been in vivo microdialysis of extracellular fluid (Darvesh et al., 2011). This technique... across the semipermeable membrane from the extracellular fluid into the perfused liquid. The probe then...sensor, dLight1.1 and dLight1.2, exhibit high (submicromolar) DA affinity and high-magnitude responses (increases...s lab has created the GRABDA sensor based on a similar strategy: a GFP variant bound to a DA receptor,...cyclase in caudate nucleus of rat brain, and its similarity to the “dopamine receptor”." Proceedings of the...

...and targeting power of CRISPR combined with the large sequence capacity of transposons open whole new ...’t cause double-stranded breaks and can deliver large sequences and even entire genes. Figure 1...template and transposon proteins (Tns) to integrate large sequences into genomic DNA downstream of gRNA-specified...Created with BioRender.com. CASTs quickly became popular bacterial genome engineering tools, with early ... al., 2019). Type I-F CASTs were found to be particularly useful. But they were barely effective at editing...., Gelsinger, D. R., Vo, P. L. H., Chen, A. T., Tolar, J., Osborn, M. J., Sternberg, S. H., & Liu, D. ...on Addgene.org Addgene's CRISPR Guide (Jump to Large Edits) CAST Plasmid Collection ...

...encoded tools, such as microbial opsins, to control cellular activities using light. In 2015, scientists combined...Charles Gersbach’s lab at Duke University developed similar systems based on the light-inducible heterodimerizing...effector (LACE) system, the Gersbach lab utilized a similar strategy to develop an optimized photoactivatable...system was able to recognize the same PAM and had similar targeting specificity as full-length Cas9 (flCas9...light on and off. As one might expect from the modular nature of Cas9, Nihongaki et al. showed that it... respectively. The use of far-red light is of particular interest since blue light has limited applications...that post-UV exposure it showed cleavage activity similar to the wild-type Cas9. This photocaging technique...

...293, the most commonly used line expresses SV40 large T antigen and is thus dubbed HEK-293T. Given that...older cell lines in the research community, many cellular research tools have been optimized specifically... viral production because they express the SV40 large T-antigen. This allows them to replicate viral plasmids...HEKs can also grow in suspension, which allows for large scalability, and can also be adapted for serum-free...two areas of research where HEK cells may be particularly useful due to their intrinsic properties. ...by mycoplasma and bacteria, but HEKs are also particularly vulnerable to viral infection. Yes, your cells...Additional resources on the Addgene blog Five Popular Model Organisms 10 Tips for Mammalian Cell Culture...

...this latest work, the team designed and screened a large library of iGluSnFR3 mutants, identifying the two...somatosensory cortex, while iGluSnFR4s produces larger signal-to-noise ratios in two-photon imaging, making... making it ideal for video recordings of large populations of synapses and deep-brain fiber photometry...most experiments. These new tools promise to be a popular choice for a variety of synaptic imaging applications...gene makes stable cell line creation a breeze. Modular by design: Key elements (promoter, tag, polyA site...edits — single-base changes, small deletions, and large insertions up to 12 kb — with efficiencies between...isolating their associated protein interactome. Molecular Cell, 84(24), 4929-4944.e8. https://doi.org/10.1016...