Harnessing Bacterial Toxins for Allelic Exchange
Type
Blog Post
...two visible blue or magenta chromoproteins (Figure 2) (Liljeruhm et al., 2018). This system is a nice confirmation...resistance cassette, and the R6K origin of replication to allow replication only in hosts carrying the bacteriophage...chromosome.
These methods roughly fall into two categories: those that rely on exogenous enzymes and those...Lambda Red recombineering, which uses enzymes domesticated from the Lambda bacteriophage, is arguably the...enzymes can also accomplish the desired genomic modification. This technique is frequently called allelic...recipient of interest. Because pDS132 cannot be replicated in the recipient, the only antibiotic resistant...exchange.
To do this, we performed the following modifications to pDS132 (Philippe et al., 2004):
Replaced...