We narrowed to 762 results for: cell

...these issues by creating immortal antibody-producing cell lines (hybridomas) that secrete antibodies in culture...reproducible production. However, even clonal hybridoma cell lines can produce more than one mAb (Bradbury et...

... life that remained unexplored. Using new single-cell sequencing approaches, the lab was able to eliminate...sample was separated into smaller samples of 5-10 cells each for sequencing, allowing for lower complexity...

...engineering project at BosLab. Once he optimizes plant cell culture and transgenesis he wants to share his knowledge... in an Open Source Lab: from CRISPR to Cats Stem Cell Models for Disease & Open Science: Interview with...

... can be used to deliver fluorescent proteins to cells as determined by the serotype. You can use these...determine if your AAVs are getting to the right cells using your particular delivery technique and conditions...

...efficient gene modification. Maeder ML et al. Mol Cell. . 2008. Jul 25. 31(2):294-301. PubMed PMID 18657511...

... that converts sucrose into a toxic metabolite. Cells that have undergone a double crossover will not ...contain sacB, allowing them to grow on sucrose. Cells that have only gone through the single cross over... from Harvard. Jake received his MD and a PhD in Cell and Molecular Biology from the University of Pennsylvania...

...delivered as a pooled library via a lentiviral vector to cell lines that either stably expressed Cas9 or that ...almost all genes involved in the production of a cell surface glycan that’s required for ricin uptake....reveals pathways underlying ricin susceptibility." Cell 152.4 (2013): 909-922. Pubmed PMID: 23394947. Pubmed...

...translation of many stop codons and detrimental effects on cell growth. The C321.ΔA strain was genomically recoded...is particularly interested in the intersection of cell signaling, metabolism, and the mighty mitochondrion..."The origins of protein phosphorylation." Nature cell biology4.5 (2002): E127-E130. PubMed PMID: 11988757...

...about synthetic promoter AAVs for cell-type expression in retinal cells Resources on Addgene.org Find ...

... a common thread at the meeting. The new Somatic Cell Genome Editing program, run through the NIH Common...Improve HDR Efficiency for CRISPR Editing in Human Cells Hands-on tips for repair template design from guest...

...predict that we’d be able to edit RNA in mammalian cells with CRISPR? I certainly didn’t, but now we can ...Improve HDR Efficiency for CRISPR Editing in Human Cells Day 3: Cpf1: Cpf1: A New Tool for CRISPR Genome ...

...with sophisticated gene expression controls. Most cell types do not respond to low-intensity light, so ...Levskaya A, Marcotte EM, Voigt CA, Ellington AD. Cell. 2009 Jun 26;137(7):1272-81. doi: 10.1016/j.cell...

...experiments possible in a wide range of organisms and cell lines. In C. elegans, one of the model organisms... efficiency. Finally, as shown in Drosophila S2 cells (Böttcher et al., 2014), inactivation of non-homologous... community. Developments in mammalian and yeast cells using modified Cas9 proteins to regulate gene expression...homologous recombination donors in cultured Drosophila cells. Nucleic Acids Research 42:e89–e89 . https://doi.org...

... is primarily a means of selection that targets cells in which homologous recombination has not occurred...ensures high editing efficiency; the only non-edited cells to survive have inactivating mutations in the Cas9...one or more genes in E. coli. and in a mammalian cell line (Qi et al., 2013). Two important features of...the bacteria are unable to resolve them, and the cells die.  This could be applied to diseases where the...for Sequence-Specific Control of Gene Expression. Cell 152:1173–1183 . https://doi.org/10.1016/j.cell.2013.02.022...

...plasmids for detecting GPCR activity in a variety of cell types. Protein Purification Optimization Kit Using...

...quantitatively evaluating antibody staining, or counting cells are now routine, open-source software projects that...portable device for studying plankton using flow cells, has become an established alternative to high-priced...

... can be used to visualize cells and to quantify targeting of particular cell types. For example, the mDlx...

...EvolvR could also be a tool for lineage tracing cells that do not tolerate double-strand DNA breaks by...researchers to trace the progeny of a single starting cell. Additionally, multiplexing of guides allows EvolvR...

...Check out additional microbiology resources Stem cell models for disease and open science: Interview with...

...ImagingResearchers: Andrea Radtke, Ziv YanevCellosaurus: A Cell Line Information DatabaseResearcher: Amos BairochViral...