...collection.   Check out the SeqWell website to learn more about our plexWell kits and services! Many ...

...Read more about COVID-19 databases and resources Learn about Addgene's work to share plasmids to all scientists...

...the best way to elute and store your plasmid DNA Learn about the different grades of plasmid DNA preps ...

... the preprint repository BioRxiv. This example clearly illustrates the power of collaboration, which despite...

...what you are capable of. You would be surprised to learn how often I see resumes from good candidates that...

...most detectable and so should be used to tag your least abundant proteins, with the blue and infrared channels...

...techniques and troubleshooting methodologies drew me to learn more about the process that was used to create this...

...should have a FMO sample for any marker without a clear positive and negative population. If you’re unsure...

...protein that helps with virion assembly and particle release from late endosomes when AAV is trafficked. Rep...

...both a screen and a selection, it is important to clearly define what type of factor you are looking for ...

...Cloning Techniques like SLIC and Golden Gate Cloning Learn How to Verify Your Plasmid Read our Plasmids 101...

...description of 2nd and 3rd generation lentiviruses, please review 2nd vs 3rd generation above . Briefly, 2nd...Ther. 2005. 5(4): 387-398. PubMed . HIV-1 Genome Nuclear Import Is Mediated by a Central DNA Flap. Zennou...and partially spliced transcripts to facilitate nuclear export. Tat in trans Second generation only; Trans-activator...stimulates the expression of transgenes via increased nuclear export. LTR Sub-components: U3 R Sub-element: TAR...

... IPTG concentrations. Higher IPTG concentration leads to higher RepA expression which results in high ...

...Your Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors Additional...

...Read More about Fluorescent Protein-Based Tools Learn Some of the Pitfalls When Using Fluorescent Proteins...

...for digestion. PCR Design primers that overlap at least 24 bp with the template and if the sequence has ...

...of antibody for research, monoclonal antibodies Learn about the advantages of using recombinant plasmid-based...

...its localization in the membrane. The lesson we learned from these studies is that the tag protein can ...

...Award, which recognizes researchers who have at least one plasmid deposited at Addgene that has been distributed...

...an otherwise normal genomic background, can even lead to the formation of whole eyes from cell types that...

...through Addgene, and where all of it is likely to lead us in the future. Building circuits from CRISPRs...

... dCas9, a CRISPR protein variant lacking its endonuclease ability, to bind to genes without editing the...

... interview. Each interview is an opportunity to learn something and then apply it to the next interview...

...03239-6 Additional resources on the Addgene blog Learn about the advantages and disadvantages of using ...

...Antibodies 101: Introduction to Antibodies blog post Learn more about hybridomas Watch our Antibodies 101: ...

...strand before heteroduplex resolution, which can lead to indels (Anzalone et al., 2019). Figure...

...ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll find in this ...

...the Addgene blog Read our CRISPR 101 blog posts Learn about some considerations when designing gRNAs Find...

...currently uses ddPCR for AAV titrating. You can learn all about ddPCR for AAV titration and find helpful...

... your research! If they do (or if they don't!), please consider sharing your experience with other researchers...