We narrowed to 1,015 results for: LEA

...both a screen and a selection, it is important to clearly define what type of factor you are looking for ...

...techniques and troubleshooting methodologies drew me to learn more about the process that was used to create this...

...protein that helps with virion assembly and particle release from late endosomes when AAV is trafficked. Rep...

...should have a FMO sample for any marker without a clear positive and negative population. If you’re unsure...

...Cloning Techniques like SLIC and Golden Gate Cloning Learn How to Verify Your Plasmid Read our Plasmids 101...

...Award, which recognizes researchers who have at least one plasmid deposited at Addgene that has been distributed...

...an otherwise normal genomic background, can even lead to the formation of whole eyes from cell types that...

...for digestion. PCR Design primers that overlap at least 24 bp with the template and if the sequence has ...

...of antibody for research, monoclonal antibodies Learn about the advantages of using recombinant plasmid-based...

...its localization in the membrane. The lesson we learned from these studies is that the tag protein can ...

... IPTG concentrations. Higher IPTG concentration leads to higher RepA expression which results in high ...

...Your Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors Additional...

...Read More about Fluorescent Protein-Based Tools Learn Some of the Pitfalls When Using Fluorescent Proteins...

...through Addgene, and where all of it is likely to lead us in the future. Building circuits from CRISPRs...

...strand before heteroduplex resolution, which can lead to indels (Anzalone et al., 2019). Figure...

...ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll find in this ...

... dCas9, a CRISPR protein variant lacking its endonuclease ability, to bind to genes without editing the...

... interview. Each interview is an opportunity to learn something and then apply it to the next interview...

...03239-6 Additional resources on the Addgene blog Learn about the advantages and disadvantages of using ...

...Antibodies 101: Introduction to Antibodies blog post Learn more about hybridomas Watch our Antibodies 101: ...

...targets may not result in equivalent cleavage efficiency. In fact, cleavage efficiency may increase or decrease...Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning...decrease expression of a target gene? Once you have a clear understanding of your experimental goal, you are...also important to consider factors that maximize cleavage of the desired target sequence, or on-target activity...exhibits the lowest immunogenicity and are one of the least toxic methods for in vivo viral delivery. Additionally...Before proceeding, we recommend asking labmates/colleagues, searching the literature, or discussing with...Cas9 expression or (2) a lack of efficient target cleavage in cells that do express both Cas9 and gRNA. Before...

... your research! If they do (or if they don't!), please consider sharing your experience with other researchers...

...be packaged in a variety of capsids. To date, at least 12 different capsids, or serotypes, have been identified...

...preferences. It sounds complicated but has a pretty low learning curve! Restraint: scruffing Location: throat Gauge...

... cells, and its lifecycle ends in cell lysis, releasing the virus to the supernatant. The virus will reach...

...the fluorescent signature upon antibody binding, leading to discrepancies during unmixing. Additionally,...

...science happens in a vacuum!  Munro and Pelham learned of these new antibodies and identified Myc1-9E10...

...compensation values later. This can be helpful as you're learning! Ashlyn has a research background in CD8+ T cells...

...currently uses ddPCR for AAV titrating. You can learn all about ddPCR for AAV titration and find helpful...

...the Addgene blog Read our CRISPR 101 blog posts Learn about some considerations when designing gRNAs Find...