We narrowed to 1,042 results for: URE

...triplicate.  The nice thing about qPCR is that since it measures relative quantification - that is, since you’re...comparison of assays for accurate copy number measurement of the low-affinity Fc gamma receptor genes FCGR3A...

...here, the SMASh tag does offer a few other unique features. If your protein is not amenable to being tagged...effective it will be, given the diversity of protein structures, interacting partners, and localization. Thankfully...

... 350(13): p. 1353-6. PubMed PMID: 14999088. 7. Turetsky, T., et al., Laser-assisted derivation of human...cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell, 2006. 126(4): p. 663...

...presentation. It can help soothe your nerves and make sure you cover everything you need to during the interview...students; an online meeting can take some of that pressure off. This question and answer process can also...

...thousands of investigators studying them, how do we ensure that the research community has access to all of...24651010. 3. Ledford, Heidi. "The ras renaissance." Nature 520.7547 (2015): 278-280. PubMed PMID: 25877186...

...much more predictable than NHEJ. As seen in the figure above, short (5-25 bp) regions of homology flanking...knocking the GFP-Puro cassette into the locus (see figure below). The double-MMEJ strategy looks very similar...

... two sgRNAs isn’t feasible for your experiment, ensure your sgRNA sequence has little homology to other...mediates efficient homology-directed repair and temperature-controlled genome editing. Nat. Commun. 2017 ...

... a little assistance at some point during the procedure. Be an active partner Everyone’s learning style...printed protocol, or maybe you would like to take pictures/videos of a machine set up while your mentor is...

...Adeno-associated virus (AAV) has many features which make it a great viral vector, but its packaging ...in mice. Expression from the hybrid vector, as measured by β-galactosidase activity, was 81% of that from...

...process that will avoid such miscommunications in the future. Assumptions: Making incorrect assumptions can...Resolving conflicts is great, but avoiding unnecessary future conflicts while giving people the tools to resolve...

...-based applications in your experiment...but not sure where to start or what your options are? Addgene's...

...101 covers everything from plasmids parts and nomenclature to popular cloning techniques and troubleshooting...

...milestones Addgene is poised to reach in the near future! We’re almost at:  100,000 different plasmids ...

...parts of every quick tip video on our TikTok! Be sure to subscribe to our YouTube channel for quick tips...

...equipment to learning about how to use a tissue culture hood and other lab equipment, we’re here for you...

...to our collections pages, to comb through the literature for potential new deposits, and to meet with ...

...photos and slideshows shared some of the moments we captured over the years, including an album of Addgenie...

...you inspired by our 2017 #Deckthelab winners? Be sure to enter our 2018 #Deckthelab contest when the next...

...certain export control exceptions were made by the US Bureau of Industry and Security, pursuant to the international...

...Resources from the Addgene Blog Visit our CRISPR featured topic page Check out our Hot Plasmids Podcast ...

...your favorite one. If you have any suggestions for future GIFs, feel free to let us know on Twitter @addgene...

...export control licenses is available through the US Bureau of Industry and Security. If you have any questions...

...Microbiology Microbe meeting here in Boston next week - be sure to come by booth #851 and say hello! Just in time...

... her incredibly important role in solving the structure of DNA. 2. The Immortal Rap of Henrietta Lacks...

...scientists from a variety of different fields. Exposure - With over 100,000 views each month, the Addgene...

... its own (highly Tweetable!) audio clip. In the future, you’ll be able to access these snippets here or...

...some behind-the-scenes peaks of Addgene’s people, culture, and workplace. Rumor has it that a few Addgenies...

...improved site! Here are some of the exciting new features on the blog that will give you a better experience...

... and volume, and submit your request.    Figure 1: The AAV Packaged on Request banner.   We...

...it's an inhibitory opsin!) and due to its bistable nature, it enables inhibition of synapses distant to the...