We narrowed to 954 results for: app

1. Experimenting in an Open Source lab: from CRISPR to Cats

   Type

   Blog Post

   ... in biology but don’t really know how to start applying their skills to it. The Kittybiome project gives...

2. AAV Vector Quality Control: Going the Extra Mile with NGS

   Type

   Blog Post

   ...suspected, we proceed to de novo assembly of the unmapped reads, employing our bioinformatics software to...

3. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

   Type

   Blog Post

   ...but have run into a wall trying to study it? It happens. Is it an evolutionarily conserved gene? Can you...

4. Transferable Skills Guide: Managing a Team

   Type

   Blog Post

   ... couple of weeks now”. I had no idea this was happening even though I met with X on a weekly basis! The...

5. Common Injection Routes in Mice

   Type

   Blog Post

   ...expertise available in your lab or organization, and approvals from the organization overseeing your work. Good...

6. Anatomy of a Plasmid Page at Addgene

   Type

   Blog Post

   ...those flames about? Flame logo: The flame that appears beside some of Addgene’s plasmids is an indication...

7. Genome-wide Screening Using CRISPR

   Type

   Blog Post

   ...negatives – As with any experiment, the use of appropriate controls, multiple replicates and several cell...

8. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

   Type

   Blog Post

   ...substantial. It's estimated that genetic engineering as applied to products on the domestic market generates $300...

9. CRISPR 101: RNA Editing with Cas13

   Type

   Blog Post

   ...Addressing the potential use of RESCUE in therapeutic applications, the authors tested various dRanCas13b truncations...

10. Negative Can Be Positive: Open AAV Data with Addgene

    Type

    Blog Post

    ...this information can be cited, tracked, and appropriately credited, broadening the knowledge base of AAV...

11. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type

    Blog Post

    ...the same drawbacks to each reagent type, so this approach is not suitable for all antibodies. Detergents...

12. DNA Purification Without a Kit

    Type

    Blog Post

    ...rather a glass powder slurry that has a milk-like appearance. To make DNA stick to the glass particles in ...

13. Simplify Cloning with in vivo Assembly

    Type

    Blog Post

    ...protocol prior to transformation (Figure 1). As this approach requires no special reagents or purification kits...

14. Plasmids 101: Protein tags

    Type

    Blog Post

    ...deciding on which one to use will depend on your application (see table below). The antibodies available for...

15. Save Time with Transient Plant Leaf Transformations

    Type

    Blog Post

    ...placed in an Agrobacterium solution and a vacuum is applied with a vacuum bell or desiccator. Successful infiltration...

16. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type

    Blog Post

    ...Often times, a gene gets its name based on what happens to an organism when a gene/protein is depleted ...

17. CRISPR-mediated Plant Base Editors

    Type

    Blog Post

    ...question probably comes to mind: can CRISPR be applied to fix the defective base precisely? Until 2016...

18. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type

    Blog Post

    ...to dramatically increase the number of CRISPR applications. The first non-Cas9 CRISPR protein adapted for...

19. Plasmids 101: Stringent Regulation of Replication

    Type

    Blog Post

    ...carefully regulated to ensure that it occurs at the appropriate time—for example, the cell must have enough nutrients...

20. Twenty Years of Sharing: Expanding Accessibility

    Type

    Blog Post

    ...like viral preps and libraries were kept at the appropriate temperature for the duration of their journey...

21. Filming Science Videos in the Age of Social Distancing

    Type

    Blog Post

    ...refining the process down to a place I could be happy with it. Cycle 5 - The Future! Figure 5: ...

22. An Integrin Antibody Toolkit from IPI

    Type

    Blog Post

    ... in flow cytometry. Based on the established applications of the original hybridoma monoclonals, we anticipate...

23. Thoughts on the Future of Research 2015 Boston Symposium

    Type

    Blog Post

    ...many of the people who work in them are incredibly happy with their jobs. One of the Saturday Hack Day projects...

24. Plasmids 101: Common Lab E. coli Strains

    Type

    Blog Post

    ...common lab strain and determine whether it is appropriate for propogating your plasmid or carrying out ...

25. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type

    Blog Post

    ...international mentor creates a closer and more approachable relationship for the mentee, where (s)he feels...

26. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type

    Blog Post

    ...under a fluorescent microscope, the mycoplasma DNA appears in the form of small clusters, apart from the cellular...

27. Protocol - How to Create a Bacterial Glycerol Stock

    Type

    Protocol

    ... plate . Grow your bacteria overnight at the appropriate temperature. Growth conditions, including copy...

28. Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs

    Type

    Protocol

    ...requirements. Conclusion Although simple, following appropriate PPE protocol goes a long way. It is the easiest...

29. Protocol - Over-Agar Antibiotic Plating

    Type

    Protocol

    ...over-agar Plate shows a lawn of E. coli and no apparent selection. 150 µL of 1 mg/mL Carbenicillin plated...

30. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

    Type

    Protocol

    ...years to come. Design Briefly, we will design overlapping oligos that once annealed can be cloned directly...