We narrowed to 949 results for: app

...negatives – As with any experiment, the use of appropriate controls, multiple replicates and several cell...

...substantial. It's estimated that genetic engineering as applied to products on the domestic market generates $300...

...Addressing the potential use of RESCUE in therapeutic applications, the authors tested various dRanCas13b truncations...

...this information can be cited, tracked, and appropriately credited, broadening the knowledge base of AAV...

...the same drawbacks to each reagent type, so this approach is not suitable for all antibodies. Detergents...

...rather a glass powder slurry that has a milk-like appearance. To make DNA stick to the glass particles in ...

...protocol prior to transformation (Figure 1). As this approach requires no special reagents or purification kits...

...deciding on which one to use will depend on your application (see table below). The antibodies available for...

...to dramatically increase the number of CRISPR applications. The first non-Cas9 CRISPR protein adapted for...

...placed in an Agrobacterium solution and a vacuum is applied with a vacuum bell or desiccator. Successful infiltration...

...Often times, a gene gets its name based on what happens to an organism when a gene/protein is depleted ...

...like viral preps and libraries were kept at the appropriate temperature for the duration of their journey...

...carefully regulated to ensure that it occurs at the appropriate time—for example, the cell must have enough nutrients...

...question probably comes to mind: can CRISPR be applied to fix the defective base precisely? Until 2016...

... in flow cytometry. Based on the established applications of the original hybridoma monoclonals, we anticipate...

...refining the process down to a place I could be happy with it. Cycle 5 - The Future! Figure 5: ...

...many of the people who work in them are incredibly happy with their jobs. One of the Saturday Hack Day projects...

...common lab strain and determine whether it is appropriate for propogating your plasmid or carrying out ...

...international mentor creates a closer and more approachable relationship for the mentee, where (s)he feels...

...under a fluorescent microscope, the mycoplasma DNA appears in the form of small clusters, apart from the cellular...

... plate . Grow your bacteria overnight at the appropriate temperature. Growth conditions, including copy...

...requirements. Conclusion Although simple, following appropriate PPE protocol goes a long way. It is the easiest...

...over-agar Plate shows a lawn of E. coli and no apparent selection. 150 µL of 1 mg/mL Carbenicillin plated...

...years to come. Design Briefly, we will design overlapping oligos that once annealed can be cloned directly...

... these cells can still be used in downstream applications. Safety Warnings Lentivirus is generally considered...

...), but any HEK293T cell line should work. This approach can be adapted for different cell lines and different...

...antibody can be purified for use in a variety of applications. Sharing speeds science. We believe that sharing...

...amplified. When these reagents are combined in an appropriate buffer, a series of heating (denaturing) and ...

...~3.3 difference Ct for a 10-fold dilution is appropriate). Quality of duplicates: Exclude duplicates from...

...want a recipient plasmid to insert ratio of approximately 1:3. Since the number of base pairs for each...