We narrowed to 730 results for: ACE
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TypeBlog Post...research plan Most grant applications restrict the space available to describe the proposed work. Thus, every...
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Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog Post...reminded of the extent that C. elegans researchers embrace open science and share resources and tools. That... -
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog Post...create knockout mice include a reporter gene, which replaces the targeted gene and provides a way to track ... -
Adenoviral Vector Production and Troubleshooting
TypeBlog Post...chloride (CsCl) density gradients combined with ultracentrifugation to separate rAdV from other cellular debris... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog Post...method, dCas9 is fused to CBP, an histone acetyltransferase domain capable of rearranging chromatin structure... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post... viruses. For example, an Acr gene was used to replace genes in the difficult-to-engineer Sulfolobus islandicus... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog Post... contained all the goldilocks antibodies in one place... well, lucky for you one is in the making by Dr... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...fragment An enhanced recombinant amino-terminal acetylation system and novel in vivo high-throughput screen... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post... godsends. Tethering dCas9 to the histone acetyltransferase p300 or the DNA methyltransferase DNMT3A allows... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog Post...opportunity is the AAAS Mass Media Fellowship, which places science students for 10 busy weeks at media outlets... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post...Miyawaki et al., 1997), was designed to measure intracellular calcium and published in 1997. Since that time... -
CRISPR in the Clinic
TypeBlog Post...for these diseases was sponsored by Vertex Pharmaceuticals and CRISPR Therapeutics. Data shared from these... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog Post...this spread until vaccine programs can be put in place to provide community protection. Toehold switches... -
Plasmids 101: FLEx Vectors
TypeBlog Post...gene “GeneR”). The key to this experiment is the placement of the two pairs of target sites. By flanking ... -
Tips for Titering Your Lentiviral Preps
TypeBlog Post...or single cell sorting based on expression of a surface marker, subcloning, expansion, and confirmation... -
7 Simple Strategies to Resolve Conflicts with Difficult Supervisors
TypeBlog Post...both personal and professional conflicts. At the workplace, these strategies will help you to communicate... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog Post...guide sequence to every possible 23bp sequence (protospacer + PAM) in the target region after HDR. If the... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog Post...In many tissues, stem cells serve to replenish/replace damaged cells that no longer function adequately... -
Plasmids 101: Degron Tags
TypeBlog Post... is added in the form of auxin hormone indole-3-acetic acid (IAA), the TIR1 F-box associated E3 ligase... -
The AAVantages of AAV in CRISPR Screens
TypeBlog Post...that when using Cas9, you’re already almost out of space! Don’t let this get you down for too long, though...