We narrowed to 725 results for: ACE

1. Mouse Modeling, Part 1: Genetically Engineered Mice

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   ...create knockout mice include a reporter gene, which replaces the targeted gene and provides a way to track ...

2. Adenoviral Vector Production and Troubleshooting

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   ...chloride (CsCl) density gradients combined with ultracentrifugation to separate rAdV from other cellular debris...

3. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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   ...method, dCas9 is fused to CBP, an histone acetyltransferase domain capable of rearranging chromatin structure...

4. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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   ... viruses. For example, an Acr gene was used to replace genes in the difficult-to-engineer Sulfolobus islandicus...

5. Corralling and Cornering the Goldilocks Antibody

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   ... contained all the goldilocks antibodies in one place... well, lucky for you one is in the making by Dr...

6. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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   ...fragment An enhanced recombinant amino-terminal acetylation system and novel in vivo high-throughput screen...

7. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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   ... godsends. Tethering dCas9 to the histone acetyltransferase p300 or the DNA methyltransferase DNMT3A allows...

8. CRISPR in the Clinic

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   ...for these diseases was sponsored by Vertex Pharmaceuticals and CRISPR Therapeutics. Data shared from these...

9. Adapting Toehold Switches to Detect Zika Virus

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   ...this spread until vaccine programs can be put in place to provide community protection. Toehold switches...

10. Plasmids 101: FLEx Vectors

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    Blog Post

    ...gene “GeneR”). The key to this experiment is the placement of the two pairs of target sites. By flanking ...

11. Tips for Titering Your Lentiviral Preps

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    Blog Post

    ...or single cell sorting based on expression of a surface marker, subcloning, expansion, and confirmation...

12. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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    ...both personal and professional conflicts. At the workplace, these strategies will help you to communicate...

13. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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    Blog Post

    ...guide sequence to every possible 23bp sequence (protospacer + PAM) in the target region after HDR. If the...

14. Plasmids 101: Degron Tags

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    Blog Post

    ... is added in the form of auxin hormone indole-3-acetic acid (IAA), the TIR1 F-box associated E3 ligase...

15. The AAVantages of AAV in CRISPR Screens

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    ...that when using Cas9, you’re already almost out of space! Don’t let this get you down for too long, though...

16. Of Myc and Men

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    ... one of several new tools for unraveling the multifaceted role Myc plays in human health and disease. ...

17. Academic vs. Industry Postdocs

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    Blog Post

    ...better understanding of the fast, deadline-driven pace of biotech. In other words, industry postdocs already...

18. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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    ...In many tissues, stem cells serve to replenish/replace damaged cells that no longer function adequately...

19. Google Forums Round Up: First Impressions of NgAgo

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    Blog Post

    ...community if you've had success with it. Some great places to get your results out there include: Google ...

20. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    Blog Post

    ... is sedimentation equilibrium analytical ultracentrifugation [2]. Some affinity assays used in the papers...