We narrowed to 712 results for: BASE

1. Hot Plasmids: Winter 2025

   Type

   Blog Post

   ...Garcia-Marcos’ lab recently developed a collection of BRET-based ONE vector G-protein Optical (ONE-GO) biosensors...

2. Magnetic Control of Proteins: More than a Dream

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   Blog Post

   ...could be harnessed or enhanced for future MagLOV-based tools, just as light-induced conformational changes...

3. Viral Vectors 101: The Retroviral Lifecycle

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   Blog Post

   ...Lentiviral Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives. Viruses...

4. Viral Vectors 101: Preparing Pooled Libraries

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   Blog Post

   ...followed by sequencing and further data analysis. Based on Figure 31.5.2 from https://pubmed.ncbi.nlm.nih.gov...

5. Antibodies 101: The Four ELISAs and When to Use Them

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   Blog Post

   ...simplicity we will discuss the more common enzyme-based reporter methods. For enzymatic signal detection...

6. CRISPR 101: Making a Knock-In Cell Line

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   Blog Post

   ...Learn about Non-Homologous End Joining Learn about Base Editing Read 3 Tips to Improve HDR Efficiency for...

7. An Inside Look into Addgene's Dev Team

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   Blog Post

   ...development called Scrum. The Scrum framework is based on an iterative and collaborative approach to development...

8. Droplet Digital PCR for AAV Quantitation

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   Blog Post

   ...particularly important if your amplicon is longer than 150 base pairs. While we use ddPCR for AAV titration, there...

9. Transferable Skills Guide: Career Planning Resources

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   Blog Post

   ...you do? What follows are just a few suggestions based on what I have found to be especially beneficial...

10. Healthcare Consulting: A Door to the Business of Life Sciences

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    Blog Post

    ...resources towards the development of a new therapy based on its market need and anticipated commercial success...

11. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    Blog Post

    ... 1a). Other strategies that have been developed based off of this concept include: Self-labeling tags...

12. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type

    Blog Post

    ...Ohashi, T., et al., An experimental study of GFP-based FRET, with application to intrinsically unstructured...

13. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    Blog Post

    ...whether you will create an N- or C-terminal fusion based on your experimental needs. Equally important, before...

14. Data Freedom: The Expansion of Data Sharing in Research Publications

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    Blog Post

    ... it, could allow idea generation and extension, based on existing, published data. The importance of data...

15. Using AAV for Neuronal Tracing

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    Blog Post

    ... neuron can then be traced throughout the brain based on its unique hue (plasmids at Addgene). A large-scale...Its efficiency is comparable to conventional dye-based tracing while also having the added advantage of...

16. Pouring LB Agar Plates

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    Protocol

    ... make. The precise mass you measure out will be based on the number of plates you’d like to pour. For ...

17. AAV Titration by qPCR Using SYBR Green Technology

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    Protocol

    ...physical titer of samples (viral genomes (vg)/mL) based on the standard curve and the sample dilutions. ...efficiency of PCR) ~100% (90%–110% range is acceptable) Baseline removal: all samples will have some small amount...

18. Isolating a Monoclonal Cell Population by Limiting Dilution

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    Protocol

    ...monoclonal lines generated using this protocol will vary based on several factors, including the growth properties...

19. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

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    Protocol

    ... 2: Buffer exchange Choose Option 1 or Option 2 based on the concentration of the pooled sample above....

20. Allen Institute for Brain Science AAV Enhancer Collection

    Type

    Collection

    ...in mice. Enhancers were selected from the genome based on their differential chromatin accessibility across...