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  4. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...amino acids. This is possible because there are 4 bases (A, T, C, and G) and 3 positions in each codon. ... at all in E. coli (you can find a variety of databases showing various organisms’ codon preferences online...

  10. Cre-lox system

    Type
    Collection
    ... out Addgene’s Plasmids 101: Cre-lox blog post. Based on these Cre-lox recombination principles, scientists... pK170.AAV-TRE-Cre-WPRE (Supernova) TRE-Cre based Supernova TRE AAV Iwasato 85577 pTC-CMV-Tet CreER...

  11. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ... neuron can then be traced throughout the brain based on its unique hue (plasmids at Addgene). A large-scale...Its efficiency is comparable to conventional dye-based tracing while also having the added advantage of...

  12. Deisseroth INTRSECT Collection

    Type
    Collection
    ...virus (AAV)-borne payloads to be expressed in cells based on a doubly-specified combination of genetic and...

  13. Pouring LB Agar Plates

    Type
    Protocol
    ... make. The precise mass you measure out will be based on the number of plates you’d like to pour. For ...

  14. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...physical titer of samples (viral genomes (vg)/mL) based on the standard curve and the sample dilutions. ...efficiency of PCR) ~100% (90%–110% range is acceptable) Baseline removal: all samples will have some small amount...

  17. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...genomic edit. Some Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to ...from Staphylococcus aureus (SaCas9) is about 1 kilobase smaller than SpCas9, so it can be packaged into...

  19. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ... in the envelope and are targets for complement-based killing by anti-alphaGal antibodies. The VSV-G envelope...
Showing: 601 - 620 of 706 results