...make a strategy that can help. Even something as simple as telling yourself, “I can stop and check my notes...Most of the time, that’s enough–but if it’s not, I can always take more time to check my notes!  Know your... speaking, Murphy’s law definitely applies: what can go wrong, will go wrong. The trash removal service...to,” or “It is okay if it doesn’t go perfectly,” can help. (And do remember to breathe!)   Public speaking... a chance to comfortably engage with you. If you can give a seminar, lead a journal club, or present at.... The whole process took just under a minute. I can't say the journal club was a rousing success–the response...

...assembled, the four chains make a Y-shaped protein that can be divided into the Fragment Crystallizable region...allows them to get into samples more efficiently and can also improve localization accuracy when used in super-resolution... to use Fab fragments as primary antibodies, you can make your own antibody fragments as needed.  In contrast...complex into the sample. Alternatively, Fab fragments can be used as a block in similar scenarios. By applying... here? Many of the Fab fragments described above can be used for similar applications and have similar...interchangeable, but the extra bit of heavy chain on a Fab’ can act as a convenient site for conjugation.  Finally...

...or post-mortem experiments. Fluorescent proteins can also be identified using antibodies and immunohistochemistry... always be the best option. Fluorescent proteins can interfere with fused protein function Under some ...some circumstances, the fused fluorescent proteins can be a roadblock, rather than an aid, in the expression...., 2013). We found that the fluorescent proteins can, indeed, interfere with the correct function of the...learned from these studies is that the tag protein can impact the expression of the transgene of interest...provided evidence that some fluorescent proteins can be, in some circumstances, detrimental to the expression...chemogenetic receptors used in these techniques cannot be directly visualized with fluorescence or immunohistochemistry...

...buy-in from all of Addgene’s teams. This solution can easily be implemented in an academic lab setting,...ambiguous terms. Defining words and applying annotation can be particularly useful in academic labs when creating...Clarify: Asking for clarification during a meeting can help get everyone on the same page. Others in the...meeting might also have the same question and asking can help the team identify possible points of confusion... done. But if you implement it well, here’s what can happen: More effective meetings. When everyone at...people aren’t listening to you or understanding you can be incredibly frustrating. Clarifying the words —...

...there is a clear winner, is there something that we can invest our time into learning and extract the most...CRISPR, there are two major areas where software can help. The foremost handles the design of sgRNA and...but is also a good example of how database tools can be used to aid design. These databases are still ...approximate methods. The details of their scoring function can be found in their online tool’s documentation. Since...the tool (a screen shot and overview of the table can be found above). These categories are further discussed...concern than data output as different input types can be readily converted.  Examples: “organism”, “sequence...provide results to the user in different formats. This can have a large impact on downstream results. Examples...

...literacy as I believe people that can see and understand data themselves can come to conclusions themselves...correctness of the hypothesis. The results of experiments can help support or reject a hypothesis. Looking at the... we learn about reality. Formally, a government can be defined as a social contract citizens enter into...drop them. Scientific realities limit what policy can do and, in this way, science has always been related...not make it happen. In a time when a top official can be quoted as saying “I’m not in the job of having...Washington as well as the satellite marches. Anyone can join the marches or support the cause. For science...to contact representatives at the state level you can click on your state in this map. Finally, the march...

...properties of state-of-the-art probes, many of which you can find at Addgene. Biliverdin chromophore Near-infrared...Shemetov et al, 2017). Modern state-of-the-art NIR FPs can be used similar to GFP-like probes by supplying a...the best for working in this spectral range. You can also check that no filters and/or cold mirrors block...multiplexing (Shcherbakova et al., 2018). NIR FPs can be combined with blue-light controlled optogenetics...based on light-oxygen-voltage (LOV) domain. They can also be used in crosstalk-free three-color imaging...red-shifted light. The same NIR FPs and biosensors can be imaged at spatial scales from sub-cellular to ...the maxima of their emission spectra. These probes can be combined in two-color and multicolor NIR imaging...

...requested AAV prep for the 2nd year running, and you can learn more about this useful, DREADD-containing AAV...recombinase under the control of the synapsin promoter. It can be used to drive Cre expression specifically in neurons...efficiently deliver genes to various brain regions. You can partially control which cells are successfully infected...interest is expressed.  A cell-type specific Cre can provide you with that control in a modular way. ...

.... The lab demonstrated that XMAS-TREE can be multiplexed and can be used in human pluripotent stem cells...MYC-ChIP-seq data from several MYC-dependent cancer cell lines. It can be applied for identification of essential...  After identification of ABE activity, XMAS-TREE can also be used to purify these modifications and work...with one sgRNA per operon or gene. The sgRNA pool can also target core operons in other pneumococcal strains...

...greater than 1.5. Then, using the A260 reading, you can calculate the DNA concentration. Generally, A260 ... their corresponding fluorescence. That way, you can compare the fluorescence of your sample against this...Though not the quickest way to quantify DNA, you can use the agarose gel method to not only find out how... acidic conditions and forms a blue complex that can be quantified at 595 nm. This method is time consuming...the measurements are made in the visible range and can be read with a standard ELISA reader if no other ...sensitivity at low concentrations of DNA and it can’t distinguish between DNA and RNA. See our protocol...This is something that absorbance based methods can’t tell you and you don’t need a spectrophotometer ...

...multiple AAV lots can be run in parallel and provide serotype data in minutes and the scripts  can be easily ... 100 AAV vector preparations and showed that VGS can quickly and reliably confirm the identity of AAV ...present in Cre-dependent vectors and a script that can confirm the serotype of AAV preparations.  Using ...this simplified sequencing and analysis approach can be easily incorporated into standard QC regimens ...

...courses get plenty of theory in their lectures, but it can be difficult to determine how much information they...it in an interactive setting. The problem is…how can you make a “hands-on” lesson on genetic manipulation...let your students play. As your students play, you can observe, answer questions, and guide them to productive...! Accessing and modifying BUILD YOUR PLASMID You can find rules, suggestions, and more details on how ...teaching developmental biology Learn how BioBuilder can help teach students about Synthetic Biology Find ... various diseases. That’s when I thought, if it cannot be hands-on, it will be “brains-on”! I know that...

...literature. I think that delivering constructive critique can help your colleagues at the receiving end, if not...open science in your peer-review Additionally, you can ask that data and resources described in the manuscript...journal you are reviewing for. The code and the data can be deposited in the public on-line repository (like...resources, like constructs or biological material, can be deposited in the specific repositories (Addgene... authors.” By addressing the comments to “you” I can easily check if I would have formulated specific ...

...AAV we have released in the last few months. You can also browse our entire AAV inventory. Our new AAVs...size 20 µL samples of control AAV that scientists can use for serotype testing. These samples are available...non-human primates, where fusion fluorescent proteins can affect DREADD trafficking and localization. The Sternson...encode engineered ligand-gated ion channels that can be activated with FDA-approved drugs. See our inventory...inventory. Flp is another useful recombinase that can be used in combination with Cre for intersectional...

...Crystal violet (CV) is water soluble and can enter the peptidoglycan layer of both bacteria types. In water...on the structure of a bacteria species and how it can be used in a lab? Here we’ll be talking about the...bacteria are stained red or pink. Purpose Gram staining can be used in medical settings to determine the general...bacteria present in an infection. This information can then be used to determine effective courses of treatment...plasmid to be inserted into a specific bacterium, it can be important to consider the type of bacteria it ...bacteria, or monoderms, have a single thick peptidoglycan membrane. Common gram positive bacteria include...negative bacteria, or diderms, have a thinner peptidoglycan membrane and an outer lipid membrane. Common...

...but a longer term and more substantial experience can be achieved by volunteering for a professional organization...Hopefully sharing my experience will show you how you can get started on your own development as a leader. ...become Chair of a Committee overnight (however this can be done if you already have some leadership experience...sections of the NPA’s website. By volunteering, you can gain experience working with other people mostly ...gaining transferable skills such as leadership skills can only assist you and make you more qualified for future.... Becoming involved is not too difficult and you can find opportunities around you. You just need to be...

...make a pretty good team. We have confidence that we can take on any challenge and quickly become experts ...organisms, and GMOs have a bad reputation. The public is can be fearful of this technology and angry at the agriculture...hate GMOs, but what you are doing makes sense. I can support that.” That was interesting. If people could...’s exhilarating to have that sort of freedom. It can also be overwhelming – there is so much knowledge... that the learning curve is pretty steep. But we can rely on one another to celebrate good news or share... pursuing scientific or business enterprises, he can be found climbing mountains or playing with his two...

...Organism box. This small piece of information can significantly reduce your wait time for blastn, blastp, ...sequences (blastn)” program for blastn so that you can extract practically every single reliable base pair...results. This option is not as fast as megablast, but can return longer alignments to compare with your sequencing...mismatches or gaps in the nucleotide sequence, you can run a Translated BLAST (blastx) search to check for...blastx search. An alignment omitting repeated regions can be confusing, such as when you attempt to verify ...reading frame to choose for your sequencing result and can easily translate it, we recommend using blastp over...

...researchers are using. The detailed results of his survey can be found in the full post but TLDR: many users have...school and provides a thorough walkthrough of how you can use PCR to make small sequence changes to plasmid...written in terms any programming noob (like myself) can understand. You’ll also find actionable advice on...emailing (hopefully non-vacationing) scientists. You can find all of our August posts in chronological order...

...Videos can save researchers time by quickly relaying key points about an unfamiliar topic or lab process...detailing Streaking Plates for Single Bacterial Colonies can be found below: Career videos Ever needed career...video, featuring Outreach Scientist Jessica Welch, can be found below: How-to videos Our How-To videos...