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We narrowed to 117 results for: CAN

Showing: 1 - 20 of 117 results

  1. Antibody Guide

    Type
    Collection
    ...reaction. Many antibodies can be purchased already conjugated, which can save significant time in the lab. However...choice, you can conjugate antibodies in the lab. The complexity of this can vary significantly based on ... B cell can clone itself to enable higher production of a single antibody, but each B cell can only produce... and can be used to increase flexibility or specificity in antibody-based methods. Antibodies can recognize...antibodies can be isotype-specific or even isotype subclass-specific. This specificity can be used to ...that you can detect in a single assay is limited by the number of signaling molecules that can be distinguished...may be present in. However, polyclonals can vary significantly from lot to lot, as immune responses have...

  2. Plan Your Experiment

    Type
    Collection
    ...transcriptase (RT)) Can edit within a wider window than most base editors Editing efficiency can be low, but ...within the gene itself can also be effective, as the Cas9 bound to the DNA can interfere with transcription...gRNAs can save your lab valuable time and resources when carrying out CRISPR experiments and can be used...transfection or electroporation. Expression can be transient, or you can generate stable cell lines if your plasmid...targeted edits can lead to changes in more than just the DNA sequence. If this is the case, you can confirm ...different Cas proteins available, and researchers can adapt these experiments for different organisms. ... to have a biological question in mind. Then you can decide how to answer that question using genetic ...

  3. Lentivirus Plasmids

    Type
    Collection
    ...fusion; can be used for cDNA expression; puro resistance Sabatini 14883 FUGW 3rd hUbC-driven EGFP; can be ...research. Lentivirus can transduce both dividing and non-dividing cells without a significant immune response... lentiviral envelope and packaging plasmids that can be used with 2nd and 3rd generation lentivirus technologies...Description PI 12253 pRSV-Rev 3rd Packaging Contains Rev; can be used with 3rd generation transfer plasmids along...12251 pMDLg/pRRE 3rd Packaging Contains Gag and Pol; can be used with 3rd generation transfer plasmids along...psPAX2 2nd Packaging Contains Gag, Pol, Rev, and Tat; can be used with 2nd and 3rd generation transfer plasmids...R8.2 2nd Packaging Contains Gag, Pol, Rev, and Tat; can be used with 2nd and 3rd generation transfer plasmids...

  4. AAV for Neuronal Tracing

    Type
    Collection
    ...present, the virus can spread from this cell to synaptically connected cells (meaning, it can spread to cells...Thus, G-deleted rabies can infect a single "starter" cell, but new virus cannot spread to any neighboring...rabies virus-based monosynaptic tracing. These AAV can be used with modified rabies virus to perform retrograde...Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G Can be used to complement deletion-mutant rabies virus...pAAV-syn-FLEX-splitTVA-EGFP-tTA These AAV together can be used to complement deletion-mutant rabies virus...infected cells that lack the G protein, the virus can replicate but the newly formed viruses are unable... through complementation, G-deleted rabies virus can spread to monosynaptically connected cells. To further...

  5. Zhang Lab CRISPR Page

    Type
    Collection
    ...vector can be digested using BbsI, and a pair of annealed oligos (design is indicated below) can be cloned...plasmid can be digested using BbsI, and a pair of annealed oligos (design is indicated below) can be cloned...Protocol 237.2 KB SpCas9 Plasmids The CRISPR/Cas system can be implemented in mammalian cells by co-expressing...additional guanine nucleotide to the 5’ of 20-bp guide can increase targeting efficiency. SpCas9 and SpCas9n...cassette expressing human codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons (Ran et al., Nature...Individual human codon-optimized SpCas9 plasmids can be ordered via the links below: 42230 : PX330; SpCas9... below. Pooled genome-wide human SAM library SAM can be combined with a human genome-wide library to activate...

  6. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...deaminase protein becomes a specific base editor that can alter DNA bases without inducing a DNA break. Base...reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce ...dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. Design...to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering with transcription...Type VI enzymes that function in mammalian cells can be used to attentuate RNA levels. In mammalian systems...Publication Purify A catalytically inactive Cas9 (dCas9) can be used to purify a region of genomic DNA and its...Cas9 (dCas9) fused to a fluorescent protein (FP) can help visualize specific genomic loci using fluorescent...

  7. Bacterial Expression Systems

    Type
    Collection
    ...promoter element can be used to quickly determine whether or not a particular protein can activate expression...of a split FP. When the candidate proteins interact, the pieces of the FP can get together and reconstruct...Find bacterial expression plasmids that can be used for protein purification, protein visualization, ...features. Addgene distributes many plasmids that can help researchers plan their experiments using bacterial...plasmids and produce proteins in high yields. Proteins can then be extracted from the culture and purified through...are often expressed alongside epitope tags, which can also later be used for protein detection in western.... The pCri System (Addgene #1000000058) plasmids can be utilized for heterologous cytoplasmic and periplasmic...

  8. Worm Expression Resources

    Type
    Collection
    ...plasmids can be found on the Jorgensen Lab page. More information on miniMos and MosSCI plasmids can be found...cell biology, development, and aging. Scientists can knock down genes by feeding worms bacteria expressing...table. For a more complete list of plasmids that can be used in worms, search Addgene's entire collection...Collections are plasmids grouped by a specific topic, but can only be ordered individually. CRISPR Read Addgene's...CRISPR resources, along with links to protocols, can be found below: SapTrap-SEC kit for C. elegans genome...cassette (SEC). More information regarding this kit can be found at the Goldstein Lab website . SapTrap CRISPR...expressed from pHO4d-Cas9 from Michael Nonet. Protocol can be found at the (Link opens in a new window) Seydoux...

  9. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... Cre-lox can be used to turn shRNA constructs on or off. In floxed-shRNA constructs, Cre can excise the...SSRs that cleave all four DNA strands at once. They can mediate the integration of a large DNA payload containing...that Cre-lox and other site-specific recombinases can be used to modify the genome or control gene expression...cell-specific promoters or inducible systems, you can precisely control and optimize expression of your... how the target sites are arranged, recombinases can be used for more than inversion, deletion, or translocation...For more information on FLEx switch and what you can do with it, read Addgene's blog on FLEx Vectors ....site. Multiplexing: Orthogonal recombinase systems can be combined in multiple, independent recombination...

  10. TALEN Guide

    Type
    Collection
    ...interactions between zinc fingers within an array can reduce their specificity. Two years ago, groups led...targets thymine, and NN targets guanine (though NN can also bind adenenine with lower specificity). With... is composed of 18 TAL effector repeats. The kit can be used to make arrays from 12-31 repeats. Perhaps... TAL effector arrays is the ease with which they can be made. Intuitively, one would expect that assembling...s group from the University of Minnesota, arrays can be assembled in a matter of days. In an open access...groups describe a set of customized plasmids that can be used with the recently developed Golden Gate cloning...Addgene , custom arrays consisting of 12-31 repeats can be assembled and inserted into a variety of backbones...

  11. CRISPR Guide

    Type
    Collection
    ...monocytogenes , which can inhibit the CRISPR activity of both LmoCas9 and SpCas9. Acr proteins can be used to control...desired edit can increase efficiency. Synchronizing cells or modulating the cell cycle can help as HDR .... CRISPR imaging can visualize chromatin and RNA dynamics in live cells. Labeling can be done by fusing...capabilities , as it can process multiple gRNAs under a single promoter, and can cleave ssDNA non-specifically...and quantification and can be used on lateral flow strips. Other Cas enzymes can be added to SHERLOCKv2...shredder” system that can cause large genomic deletions. The boundaries of these deletions can be defined, either... an enzymatically inactive form of Cas9; can bind, but cannot cleave DNA DSB D ouble- S trand B reak, ...

  12. Optogenetics Plasmid Collection

    Type
    Collection
    ...archaebacteria, that can be opened and closed in response to light. These proteins can be expressed in specific...Archaerhodopsins and Leptosphaeria rhodopsins, are pumps that can actively move cations or anions into or out of the...expression and localization. Many other types of proteins can also be controlled by light, generally by utilizing...light-oxygen-voltage-sensing (LOV) proteins. These fusions can then be used as an optical switch to control protein...

  13. AAV Packaged on Request

    Type
    Collection
    ...production. You can do this whenever you are ready. Payment information must be added before we can begin processing.... It has low immunogenicity, is easy to use, and can be safely handled at the lowest biosafety protection...choices! By using AAV Packaged on Request, scientists can start experiments as soon as they receive their AAV...volumes. Details Scale and Titer Packaged on Request can be ordered in four sizes. For all viral vector preparations... Scope of Service . Serotype Packaged on Request can be ordered in seven serotypes: AAV1 AAV2 AAV5 AAV6... request and we’ll let you know whether the item can be packaged. Addgene does not perform functional ...

  14. CRISPR Plasmids - Tagging

    Type
    Collection
    ...footprints, and can be scaled up for systematic editing of multiple genes. Plasmids can be found associated...details on how to use this plasmid in your experiments can be found below and in the supplemental materials ...Dalvai et al. Cell Rep. 2015 . Alternatively, cDNAs can be cloned directly into this vector and targeted ...HDR-mediated gene knock-in. The detailed procedure can be found in Sakuma et al., Nature Protocols, 2016...essential nuclear and cytoplasmic proteins, which can then be depleted very rapidly after the addition ...addition of auxin to the culture medium. Plasmids can be found associated with the following article: Natsume... for selectable genome modification. This system can be used to create C- and N-terminal epitope tags....

  15. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...protein becomes a specific cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine...reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce ...dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. Design...to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering with transcription...sequence, they adopt an enzymatically active state and can bind and cleave additional RNAs regardless of homology...Publication Purify A catalytically inactive Cas9 (dCas9) can be used to purify a region of genomic DNA and its...gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids...

  16. CRISPR Plasmids - Yeast

    Type
    Collection
    ...protein becomes a specific cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine...reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce ...dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. Design...to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering with transcription...Publication Purify A catalytically inactive Cas9 (dCas9) can be used to purify a region of genomic DNA and its...gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids...

  17. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...relatively low immunological effects in vivo . While AAVs can be handled at BSL-1, AAVs expressing oncogenes or...or toxins should be handled at BSL-2. AAV can transduce both dividing and non-dividing cells with a low...integrate into the host genome, transgene expression can be long-lived. The utility of AAV is currently limited... a helper virus. Instead, specific gene products can be provided by helper plasmids (e.g., pHelper) and...helper plasmids for packaging your AAV product. You can search or this table based on plasmid name, capsid...required to produce infectious AAV particles. You can search or sort this table based on plasmid name, ...

  18. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ...nucleases in different cell types. Zinc finger arrays can be transferred in-frame to these vectors....ZFC reagents for either OPEN or modular assembly can be easily transferred in-frame to any of these vectors... Biotech 2007 ) in mammalian cells or zebrafish (can be used as a substitute for pMLM335 described in ... Biotech 2007 ) in mammalian cells or zebrafish (can be used as a substitute for pMLM336 described in ...

  19. AAV Molecular Tools

    Type
    Collection
    ...tools/controls and tetracycline transactivators that can be used with tetracycline (tet)-inducible expression...AAV encode tools for affinity purification (which can be used for viral TRAP). ID Name Expression System...Neurophysiology Tools These AAV encode tools that can be used for neurophysiology experiments, including...Tools for Cell Ablation These AAV encode tools that can be used for cell ablation. ID Name Expression System...Zhang Labeling Tools These AAV encode tools that can be used for cell or protein labeling. ID Name Expression...mNaChBac and (physically separate) tdTomato 8 Scanziani 34910 paavCAG-pre-mGRASP-mCerulean CAG-driven,...

  20. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...level. By controlling the number of mismatches we can obtain a range of expression levels. This collection...Detailed information about the repression system can be found in this article: Vigouroux, A., Oldewurtel...information about chromosomal integration of pLC97 can be found in this article: St-Pierre, F., Cui, L.,...the two reporters, mCherry and sfgfp in this case, can be controlled using a plasmid‐borne CRISPR array ...
Showing: 1 - 20 of 117 results