We narrowed to 753 results for: ELP
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TypeBlog Post...might expect. References 1. Kaiser J. CRISPR helps heal mice with muscular dystrophy. Science. 2015...
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Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog Post...these solutions to your own research and thereby help move the biological research enterprise towards ... -
How to Design Your gRNA for CRISPR Genome Editing
TypeBlog Post... Institute and has worked with many Addgenies to help improve the understanding, curation, and explanation... -
Hot Plasmids: Spring 2025
TypeBlog Post...targeting guide and the transposon RNA homology both help direct the fusion construct to the new genomic locus... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog Post... be expressed or not expressed in each state. To help researchers adapt GRSMs for their research, we provide... -
Plasmids 101: Restriction Cloning
TypeBlog Post...good idea to run an uncut sample of each plasmid to help with troubleshooting if your digests don’t look ... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog Post...critical for success. Online tools are available to help optimize ligation reactions; the Thermostable Ligase... -
Important Considerations When Using AAVs
TypeBlog Post...research. Below I’ll provide some tips that will help you think about how to get started with AAVs. Since... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog Post...look smeared, so having an undigested control can help distinguish between a smear and a faint digestion... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog Post...kind of problem. Educate others when you can and help them understand the importance of preventing contamination... -
A History of Genome Engineering in Popular Culture
TypeBlog Post...understanding of the adenine base editors which can help accelerate the engineering of novel base editor ... -
Sequencing Options for CRISPR Genotyping
TypeBlog Post...assayed phenotype. Verifying these connections can help alleviate the reproducibility crisis in biology.... -
Which Fluorescence Microscopy Techniques is Best for Me?
TypeBlog Post... microscopist. Hopefully this brief synopsis can help point you in the right direction. You certainly ... -
Typing CRISPR Systems
TypeBlog Post...general principles of this classification system will help you gain a deeper appreciation and understanding... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog Post...expression of the transduced protein. I hope that helps you as you begin to design your first flow panels... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post...various promoters and other genetic manipulations to help restrict expression to a specific neuronal subtype... -
How to Deposit Your Plasmids with Addgene
TypeBlog Post...verified by next-generation sequencing. Please see the help center article, "Can I deposit a pooled DNA library... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog Post...again? We’re glad you asked. Hopefully this table helps you keep them straight! Innovation Purpose... -
27 Hot Plasmids from 2016
TypeBlog Post...about depositing your new plasmids with Addgene to help expand the collection! The Jacks lab has made several... -
Adeno-associated virus (AAV) Plasmids
TypeCollection...without the use of a helper virus. Instead, specific gene products can be provided by helper plasmids (e.g.,...transfer plasmid). Helper Plasmid ID Plasmid Serotype Description PI 112867 pAdDeltaF6 All Helper plasmid for...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...Traditionally, AAV requires the presence of another "helper" virus, such as adenovirus or herpes virus, in ...replication. This requirement has been circumvented with “helper-virus free systems,” which enable the production...., pHelper) and specific packaging cell lines (e.g., HEK293 cells) during AAV production. Read our AAV...In order to generate infectious AAV particles, a helper plasmid (encoding adenovirus E4, E2A and VA) and...