We narrowed to 638 results for: Erf

1. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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   ...ease of use, 2) time savings, and 3) ability to perform single site or multi-site saturation mutagenesis...

2. Replacing Paper: Tips for Choosing an Electronic lab Notebook

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   ...notes etc. Detailed records about every action performed in the lab (user, date, time, action) are required...

3. An Integrin Antibody Toolkit from IPI

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   ...integrins at non-ligand-binding sites and do not interfere with integrin function. Overall, the synthetic...

4. New Optogenetic Tools for Cytoskeleton and Membrane Control

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   ...recruiting proteins to the cell membrane isn’t powerful enough, what about altering the chemical composition...

5. Tips for Getting a Faculty Position

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   ...weeds. Check out Joanne Kamens's "10 Steps to a Perfect Science Talk." 4. The chalk talk, simple in principle...

6. CRISPR 101: Homology Directed Repair

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   ...targeted-break technology coupled with HDR is a powerful site-specific knock-in tool that can be harnessed...

7. Transferable Skills Guide: Conflict Resolution

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   ...form of (hopefully constructive) conflict. As you perform the experiments to address a reviewer’s concerns...

8. Antibodies 101: Single Chain Fragment Variables (scFvs)

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   ...functional scFvs; however, some individual scFvs performing better in one configuration than the other (Sandomenico...

9. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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   ... restriction site for linearization. This is a perfect case for the use of Gibson assembly combined with...

10. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    ...of the  Brainbow multicolor labeling system - a perfect example of the continued utility of Cre-lox. Check...

11. Plasmids 101: Common Lab E. coli Strains

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    ...gene of interest, painstakingly cloned into the perfect empty backbone, made sure to add the right tags...

12. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    ...Michael, S., Kuleshova, E., Ojala, D., Huang, C.-C., Gerfen, C. R., Schiller, J., Dudman, J. T., Hantman, A...

13. Is this the right place for me? 8 tactics for choosing a lab

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    ... Or is it teamwork that brings out your best performance and learning? Everyone’s different so give some...

14. Viral Vectors 101: AAV Variables That Matter

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    ...first amount that looks effective. Ultimately, the perfect “high titer” is one that optimizes gene expression...

15. When Fidelity Matters: A frank discussion about ligase fidelity

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    ...polynucleotide ligase and RNA capping enzyme superfamily of covalent nucleotidyltransferases." Current...

16. Quick Guide to All Things Lentivirus

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    ...important to be aware that lentiviral particles are a powerful way to transduce cells - even your own! You should...

17. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    ...Orientation) or DO (Double-floxed Orientation)) is a powerful tool that provides precise temporal and spatial...

18. A History of Genome Engineering in Popular Culture

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    ...replicants which are genetically engineered by a powerful corporation to serve as slaves.  Meganucleases...

19. Typing CRISPR Systems

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    ...become a natural successor to traditional RNA interference, as it can target and degrade select RNA transcripts...

20. Cancer and the Immune System: Deciphering the Relationship

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    ...inflammatory cytokines - interleukin (IL)-12 and interferon gamma (IFNγ) - and eliminate cancer cells. 2....