Addgene

We narrowed to 632 results for: Erf

Showing: 601 - 620 of 632 results

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Tips for Getting a Faculty Position

Type

Blog Post

...weeds. Check out Joanne Kamens's "10 Steps to a Perfect Science Talk." 4. The chalk talk, simple in principle...
CRISPR 101: Homology Directed Repair

Type

Blog Post

...targeted-break technology coupled with HDR is a powerful site-specific knock-in tool that can be harnessed...
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Type

Blog Post

...tagged protein because of its size. This type of interference is known as steric hindrance. For instance, ...
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

Type

Blog Post

... intestinal crypt, and since then has become a powerful tool for understanding cell proliferation. Inducible...
Transferable Skills Guide: Conflict Resolution

Type

Blog Post

...form of (hopefully constructive) conflict. As you perform the experiments to address a reviewer’s concerns...
Antibodies 101: Single Chain Fragment Variables (scFvs)

Type

Blog Post

...functional scFvs; however, some individual scFvs performing better in one configuration than the other (Sandomenico...
Deep Mutational Scanning with One Pot Saturation Mutagenesis

Type

Blog Post

...ease of use, 2) time savings, and 3) ability to perform single site or multi-site saturation mutagenesis...
Plasmids 101: Biotinylation

Type

Blog Post

...relatively small, water soluble molecule that does not interfere with the macromolecules it is added to. Biotin...
An Integrin Antibody Toolkit from IPI

Type

Blog Post

...integrins at non-ligand-binding sites and do not interfere with integrin function. Overall, the synthetic...
Replacing Paper: Tips for Choosing an Electronic lab Notebook

Type

Blog Post

...notes etc. Detailed records about every action performed in the lab (user, date, time, action) are required...
Adeno-associated Viruses (AAVs) for Genome Editing

Type

Blog Post

...cell types, now you know that AAVs can provide a powerful boost to genome editing experiments. We’re excited...
Transferable Skills Guide: Time Management

Type

Blog Post

...quick and useful tasks will swiftly give you the wonderful feeling of accomplishment while the longer tasks...
Using Phosphoserine to Study Protein Phosphorylation

Type

Blog Post

...generate non-phosphorylated recombinant protein and perform in vitro kinase reactions. Often, however, researchers...
Evolution of Lab Techniques

Type

Blog Post

...upon the incorporation of a ddNTP. Researchers perform four different reactions, one for each radiolabelled...
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Type

Blog Post

... restriction site for linearization. This is a perfect case for the use of Gibson assembly combined with...
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

Type

Blog Post

...of the Brainbow multicolor labeling system - a perfect example of the continued utility of Cre-lox. Check...
Plasmids 101: Common Lab E. coli Strains

Type

Blog Post

...gene of interest, painstakingly cloned into the perfect empty backbone, made sure to add the right tags...
Viral Vectors 101: AAV Serotypes and Tissue Tropism

Type

Blog Post

...Michael, S., Kuleshova, E., Ojala, D., Huang, C.-C., Gerfen, C. R., Schiller, J., Dudman, J. T., Hantman, A...
Viral Vectors 101: AAV Variables That Matter

Type

Blog Post

...first amount that looks effective. Ultimately, the perfect “high titer” is one that optimizes gene expression...
Is this the right place for me? 8 tactics for choosing a lab

Type

Blog Post

... Or is it teamwork that brings out your best performance and learning? Everyone’s different so give some...

Showing: 601 - 620 of 632 results

Results per page:

Tips for Getting a Faculty Position

CRISPR 101: Homology Directed Repair

Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

Transferable Skills Guide: Conflict Resolution

Antibodies 101: Single Chain Fragment Variables (scFvs)

Deep Mutational Scanning with One Pot Saturation Mutagenesis

Plasmids 101: Biotinylation

An Integrin Antibody Toolkit from IPI

Replacing Paper: Tips for Choosing an Electronic lab Notebook

Adeno-associated Viruses (AAVs) for Genome Editing

Transferable Skills Guide: Time Management

Using Phosphoserine to Study Protein Phosphorylation

Evolution of Lab Techniques

Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

Plasmids 101: Common Lab E. coli Strains

Viral Vectors 101: AAV Serotypes and Tissue Tropism

Viral Vectors 101: AAV Variables That Matter

Is this the right place for me? 8 tactics for choosing a lab

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