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We narrowed to 1,141 results for: Gene

Showing: 601 - 620 of 1141 results

  1. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    ...experiment. PITChing MMEJ as an Alternative Route for Gene Editing Michohomology-mediated end joining (MMEJ...template to increase efficiency of insertion and gene replacement. Care to share your favorite CRISPR ...Blugene and I represented Addgene at the recent Keystone meeting on Precision Genome Editing with Programmable...hopes to evaluate genome editing pipelines and generate a common lexicon for genome editing studies. These...desired edit, and I want to make sure that I (and Addgene) don’t forget this in the future. Here’s a list... to keep current with the latest and greatest! Addgene resources 3 Tips to Improve HDR Efficiency for ...

  2. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...neurotransmission. But with approximately 950 different human genes encoding GPCRs (Takeda S et al., 2002) and many ...2002) Identification of G protein-coupled receptor genes from the human genome sequence. FEBS Letters 520... exhaustive refinement, it was not possible to generate an optimal Rluc8-Gα construct for two of the 16...s0014-5793(02)02775-8 Additional resources on the Addgene blog Browse all of our fluorescent protein blog...Find other fluorescent biosensors Resources on Addgene.org Browse the catalog of fluorescent protein plasmids...

  3. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ... throughout the chromosome. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  4. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...sequence of a thermostable DNA ligase-encoding gene." Gene 109.1 (1991): 1-11. PubMed PMID: 1756968. 7. ...nick-closing activity of bacteriophage T4 DNA ligase." Gene 76.2 (1989): 245-254. PubMed PMID: 2753355. 12. ...blood-borne bacterial pathogens by use of a novel 16S rRNA gene PCR-ligase detection reaction-capillary electrophoresis...restriction cloning), adaptor ligation for next generation sequencing (NGS) library construction, and circularization...fragments end-to-end without the exo/polymerase generation of annealed complementary regions. Why use a... does fidelity mean for a ligase? DNA ligases generally prefer fully Watson-Crick base-paired dsDNA substrates...as a correct base pair. DNA ligases have been generally found to have a higher discrimination at the upstream...

  5. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...horizontal gene transfer, which occurs when bacteria of different evolutionary lineages share genes that can...bacteria that occurs when bacterial cells regulate gene expression in response to changes in cell density... the ASM General Meeting (now Microbe) in 2013 in Denver, CO. While I had been to the General Meeting ... were very valuable when shared with the next generation of scientists. The connection between mentors... with experience mentoring in STEM, including Addgene’s Joanne Kamens. Expanding Binning Singletons to...Binning Singletons, Inc. Additional resources on the Addgene blog Browse our blog posts on scientific conferences...

  6. CRISPR Plasmids - Xenopus

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    Collection
    ...faithfully introduce specific genomic changes. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 51306 pUC57-Simple-gRNA backbone T7...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  7. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Vector Type Promoter PI Publication Empty...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 46759 pT7-gRNA T7 BsmBI In vitro transcription...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  8. AAV Viral Preps

    Type
    Collection
    ...Adeno-associated virus (AAV) vectors are safe and useful gene delivery tools that effectively transduce many mammalian...Packaging Service overview. Function Chemogenetics DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins...Ready-to-use adeno-associated virus (AAV) available from Addgene's viral vectory packaging service. Browse constructs...preps are purified by density centrifugation. Addgene’s comprehensive high-quality quality control process...shipment. If you can't find the prep you need, try Addgene's AAV Packaged on Request service. You can choose...

  9. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ...description of the VGS process and use cases in Human Gene Therapy. Existing methods for AAV quality control...Adeno-Associated Viral Genomes in Academic Settings. Human Gene Therapy Methods 30:195–205 . https://doi.org/10.1089...and tips Resources on Addgene.org Find AAVs at Addgene Learn more about Addgene's viral service Find protocols...we partnered with Seqwell to establish a next-generation sequencing (NGS) QC platform that allows us to...methods, we showed that the DNA extract is a heterogeneous mix of single-stranded and double-stranded species...interest.   While the VGS process isn’t new to Addgene, we are excited to share the procedures and Python...Vector Preparations by Single-stranded Virus Next-generation Sequencing. Molecular Therapy - Nucleic Acids...

  10. Plasmid Cloning by PCR

    Type
    Blog Post
    ... vector that is better suited for analyzing the gene’s function. The process is shown graphically in the...figure, in which we add EcoRI and NotI sites to Your Gene of Interest (YGOI) for ligation into a recipient..., you should use a DNA analysis tool, such as Addgene’s Sequence Analyzer, to allow you to identify which...restriction site to improve cutting efficiency. You can generally add any 6 bases, but you should ensure that the...with 18bp of homology to the ORF). We now need to generate the reverse-complement of this sequence so that...that we can successfully amplify the ORF. You can generate the reverse-complement using existing software...the final product. Additional Resources on the Addgene Blog Learn About Other Cloning Techniques Catch...

  11. Fluorescent Protein Guide: Empty Backbones

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    Collection
    ...you to create fusion proteins with your gene of interest....protein (GFP) was cloned in 1992 ( Prasher et al., Gene, 1992 ), and since then scientists have engineered...tags for you to create fusion proteins with your gene of interest. These are organized By Color of the...Lab Lentiviral Plasmids - Fluorescently tag your gene for lentiviral expression Verkhusha Lab Plasmids...Addgene has assembled a collection of empty plasmid backbones with different fluorescent tags for you...Monomer? Background Fluorescent proteins are genetically encoded tools that are used extensively by life...proteins that result in a diverse set of colors. Addgene has assembled a collection of empty plasmid backbones...

  12. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...also allows for screening scFv generated from the B cell antibody genes from diseased or vaccinated humans...   Some phage display libraries screen antibody genes from B cells of immunized animals, but it's possible....1791  Deal CE, Balazs AB (2015) Vectored antibody gene delivery for the prevention or treatment of HIV ...also allows for generations of scFvs against two antigens that are impossible to generate scFvs against ...probe, but is easier and cheaper to generate since it’s genetically encoded.   Translational applications... of a tagged protein. Resources on Addgene.org: Browse Addgene’s Antibody Plasmid Collection Find antibodies...-linker-VH and VH-linker-VL configurations can generate functional scFvs; however, some individual scFvs...

  13. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...amplification kit. In this case, regions of gene S and Orf1ab gene are amplified. RPA reactions also contain...amplification by primers that target the E and the N2 gene of SARS-CoV-2. If the Cas12a-sgRNA complex binds...like CRISPR. Figure 1: An overview of the general schematic of CRISPR/Cas based COVID-19 testing ...protocol for Cas13-based detection of SARS-CoV-2 genetic material (CREST) Sensitivity: 10 copies of RNA ...of temperatures varying between 10-50°C. Visit Addgene's COVID-19 Plasmids and Resources Many thanks ...Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material. Cold Spring Harbor Laboratory. https:...20detection%20(updated).pdf Additional resources on the Addgene blog Read more about SHERLOCK and DETECTR Learn...

  14. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...Gibson assembly, imagine that you want to insert your gene of interest into a vector with a large tag at the...by using Gibson assembly, you can insert both the gene of interest and the tag sequences into the vector...CATCH enables one-step targeted cloning of large gene clusters. Nat Commun. 2015; Sep 1;6:8101. PubMed...the J. Craig Venter Institute in 2009. Here at Addgene we added this option to our drop down menu of common...which chews back the 5’ ends of the fragment, generating long overhangs which allows the single stranded...advantage is that this process makes it easy to generate wild type and mutant constructs at the same time...primer melting temperature greater than 48℃. Both Snapgene and NEB have tools that help you design primers...

  15. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...may be the hot new way to manipulate gene expression, but other gene manipulation systems remain valuable...important ways to spatially and temporally control gene expression, especially in in vivo models, and new...at least six generations. In addition to the neuronal pThy1-Brainbow constructs, Addgene also has two ...Brainbow plasmids available from Addgene! Find Brainbow Plasmids @Addgene: Brainbow 1.0, 1.1, 2.1 plasmids...coupling of Cre-lox with FLP-FRT recombination to generate additional recombination events, and the synthesis...and sea anemone mKate2.) They then successfully generated custom antibodies to each of these proteins and...analysis. To achieve even cell labeling, they generated farnesylated derivatives of the fluorescent proteins...

  16. Hot Plasmids - January 2023

    Type
    Blog Post
    ...were compared head-to-head for gene expression changes of non-target genes and RNA-seq of a nuclear (MALAT1...among the first batch of antibodies we produced at Addgene and, as you might suspect, it has been one of our... need a second GFP antibody?  Similarities:  Generated against full length GFP and converted to recombinant...always best to have options.      Fig. 2: Addgene’s western blot for mEGFP-HRas (from Plasmid 18662... [N86/8R] page for more details. Image credit: Addgene.   Find anti-GFP [N86/8R] here!   Andrews NP...

  17. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ... provide a highly versatile means of delivering genes to many different cell types with minimal immunological...effects. AAVs are used in experiments targeting genes to specific cell types in the brain, mapping neuronal...Resources at Addgene.org Addgene's Viral Service Find Retrograde AAV Browse Optogenetics Plasmids ... to our viral vector pages on the Addgene website and on the Addgene blog to get some ideas for future...Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering Primer on Optogenetics Resources... Addgene recently attended the Society for Neuroscience 2017 conference in Washington DC (#SfN17). This...newbies might want to get into the field. Find Optogenetics Plasmids for Use in Neuroscience Research Neuroscience...

  18. Chemogenetics Plasmids

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    Collection
    ...AAV preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter...Service: Chemogenetics AAV Blog: Chemogenetics Viral Vectors 101 Blog: Chemogenetics vs. Optogenetics Chemogenetics... more about chemogenetic resources available at Addgene. See our inventory of chemogenetic constructs,...Collections Chemogenetics Plasmids Chemogenetics Plasmid Collection You may also like... Chemogenetics Guide...biological processes. Addgene’s collection contains two main classes of chemogenetic receptors: Designer...luciferase and a light-sensing optogenetic element, making a bimodal opto-chemogenetic actuator. When the luciferase...your experiments, please see our Chemogenetics Guide . Chemogenetics Plasmids Browse, sort, or search ...

  19. Resolute Plasmid Collection

    Type
    Collection
    ... of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR and overexpression...engineering forms the core of the deorphanizing engine. Genetic and proteomic interactions represent the framework.... Protein production is used for assays and to generate antibody/ high-affinity ligands. Data is embedded...integrated knowledgebase that features, among others, genetic variants and disease associations. Plasmids This...overexpression pooled libraries are useful tools for genetic screening experiments. These libraries target the...

  20. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...Pancreatic and duodenal homeobox gene 1 induces expression of insulin genes in liver and ameliorates streptozotocin-induced...lentiviral vectors can be used for gene delivery Resources on Addgene.org Visit our Stem Cell Pages Find...from the patient in vitro then correct the damaged gene in the resulting iPSCs before differentiating the...PMC3987909. 6. Ban, H., et al., Efficient generation of transgene-free human induced pluripotent stem cells...Mukherjee. The ultimate goal in the field of regenerative medicine is to replace lost or damaged cells...cell is converted to a different cell type for regeneration and repair and situations where one process ...plasmids used for these methods can be found on Addgene’s stem cell page. On this page, you can also find...
Showing: 601 - 620 of 1141 results