We narrowed to 918 results for: IRE

... GC-rich and repetitive regions. These regions require additional verification experiments that can be...of Snapgene. When you download a Snapgene file directly from our website, you can open it with either ...

...only makes CRISPR screen results available to the entire biomedical research community, but also allows ...identifier, which can also be used to search the entire CRISPR database for particular screens of interest...

...companies have the potential to make a huge impact directly on waste, but also on culture. Encourage your ...posts and updates from Labconscious. If you’re inspired by this post then I challenge you to take to action...

...Your next cool experiment requires some AAV.  Where do you start? Plasmids of course! You just need three...gene The rep gene encodes four proteins that are required for viral genome replication and packaging: Rep78...

...These apurinic sites if in double stranded DNA are repaired by base excision repair, but can lead to mutations...concentration. Alternatively, if the priority is to directly use your DNA preparation in any downstream application...

...widely adopted. So thank you to the camelids who inspired this versatile family of tools, the applications...Antibodies  Secondary Nanobody Toolbox No Llamas Required - Synthetic Nanobodies Against Membrane Proteins...

..., and, as mentioned earlier, how frequently the entire group will meet. You and your collaborators should...with everyone involved can speed up the future directions of the project. The state of research is constantly...

...2 components: 1) an SB transposase, the enzyme required for catalysis of transposition; and 2) a transposon...Beauty transposase as a nonviral vector for CD19-directed chimeric antigen receptor (CAR) therapy. SB is...

...long, skinny things") and generally exuding a very-tired-but-very-excited attitude. Riding up to the resort...Montana! Though sadly I will not be attending the entire meeting, Monday's talks alone were worth the trip...

...over the sides.  These tiny gels and tiny lanes required less agar, less ethidium bromide, less stop buffer...commercial boxes were small enough to minimize the required area and buffer volume.  So, I designed my own...

...survive. Genome engineering and future directions CRISPR/Cas inspired genome editing was initially described...

...large amounts of the target protein are typically required due to the relatively low efficiency of biotinylation... version 2.0? Instruments such as an Octet may require less in vivo-biotinylated protein (total amount...

... public and retrofitting our car with a freezer wired to a charger and two deep cycle batteries for power...and workflows where CRISPR nuclease activity is desired and time or protocol steps can be saved by flexibly...

...varies. See below for additional considerations. *Requires pir gene for replication (reference). **F1 is...always pick a single colony and do not subculture directly from glycerol stocks, agar stabs, or liquid cultures...

...optimized for your application. Depending on the tag, directly imaging or tracking your protein may also be possible...is not ideal with Myc, as low pH conditions are required during the purification, which may affect protein...

...turns expression on and off with blue light.   Inspired by @UriAlonWeizmann, here my song about our paper...The original AraC system, which uses arabinose, requires many wash steps to remove arabinose making it ...

...antibody tool and therefore will grow to serve the entire scientific community.     Why recombinant antibodies.... Daniela Bourges, Addgene’s interim Executive Director. “NABOR provides a new model for the creation ...

...and control elements. Development of this tool required creating a database of common features, and devising...Empirical tests indicated that a reasonable rule is to require at least 96% sequence identity when detecting a...

...down viral genomes containing only the sequences required for viral replication and transcription as well...binding proteins, dCas9, and a modified gRNA to direct a variety of customizable functions to your DNA...

...yourself, you can now order ready-to-use virus directly from Addgene! As part of our new Viral Service...Virus production can be a frustrating process that requires a large investment of time and resources for labs...