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We narrowed to 1,015 results for: LEA

Showing: 601 - 620 of 1015 results

  2. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...nucleus in space and time, and to understanding how nuclear organization varies across the life of a cell, ...dCas9 gets a colorful makeover Even without its nuclease activity, catalytically dead Cas9 (dCas9) has ...under lower illumination settings, lowering photobleaching and phototoxicity concerns and extending the...

  3. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    ...those collaborations over the years, he and his colleagues built a Molecular Expressions collection including...much easier for scientists everywhere to get a clearer picture of that intracellular world, even as the...promote the great work of Michael Davidson and his colleagues." Acknowledgments Thank you to Michael Davidson...

  4. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    ...It’s clear that CRISPR-Cas9 technology has really changed the game for anyone looking to quickly and ... positive selection screening. They and their colleagues including Feng Zhang used the GeCKO library to...CRISPR libraries developed by MIT's Tim Wang and colleagues, including David Sabatini and Eric Lander, comprise...

  7. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...CRISPRi). A) Lentiviral vector design. B) Strategy for leak-free transcriptional control. C) Iterative transduction...activation in plants.  FnCas12a, unlike other Cas12a nucleases identified, functions at temperatures up to 43...The guides are optimized to maximize on-target cleavage and minimize off-target effects.   New from ...

  8. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...cells expressing Fcγ receptors binds to IgG and release cytotoxins that kill the target cell. If the pathogen...antibodies and eosinophils, where the eosinophils release cytotoxins via degranulation.  Antibody-dependent... as phagocytosis.  Other functions We’re still learning about Fc effector functions and all that antibodies...

  9. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...scientific data depends on validated reagents along with clear documentation, so we were excited when Amos Bairoch... cited. If the information seems incomplete or unclear, Bairoch follow up via email to the authors of ... you’re excited about this resource and want to learn more, check out the Cellosaurus FAQ! References...

  11. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ...require the use of specific sequences. Read on to learn how easy this process is and to get tips for applying...not well understood, it is believed that the exonuclease activity of the polymerase generates sticky ends...fidelity DNA polymerases like phusion. An 18-cycle PCR leads to the most colonies after transformation. When ...

  12. Advice for Choosing a Research Project

    Type
    Blog Post
    ...asking what approach will I take and what can I learn, learning the difference between good and bad data, and...establishes your professional brand. It offers colleagues insight into your interests and will determine...

  14. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...suggesting that outcomes are already determined by at least day 13. However, distinct transcriptional states...335547. Additional resources on the Addgene blog Learn more about induced pluripotent stems cells (iPSCS...delivery of reprogramming factors for generating iPSCs Learn how fluorescent proteins help researchers study ...

  15. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ... nearing deadline (an animated video depicting nuclear fission; a podcast with a genetics researcher on...condensed into two discernible paths. The first leads into the world of industry. Along this road, science...Understanding just how the public utilizes insights gleaned from scientific research is, after all, one of ...

  16. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    ... A diagnostic digest is one of the easiest and clearest ways to determine if your sample has recombined...extraction to isolate the full, intact plasmid. Please see our gel extraction protocol for more details...more molecular biology and viral vector protocols Learn about the common lab E. coli strains Find tips for...

  18. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...hope that this provides a clear overview of what you need to submit data. Please note that the data hub ...generated with a caption. For microscopy images, please include channel imaged, a color legend for probes...

  19. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...viral vectors. Here are some of the AAV we have released in the last few months. You can also browse our...that govern such activities as neurotransmitter release is vital in studying neural networks. We have expanded...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...

  20. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...with lentiviral preps for the delivery of Cas9 endonuclease, Cas9 nickase, Cas9 activators, Cas9 repressors...expertise you’d like to contribute to the blog, please email us and we’ll get you writing!     www.addgene.org... Service or any of the materials we distribute, please send an email to [email protected]. Your questions...
Showing: 601 - 620 of 1015 results