We narrowed to 910 results for: Nes
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TypeBlog Post...biggest changes, there are plenty of less obvious ones to discover as you browse. You may find one of your...choices informed by Web Content Accessibility Guidelines (WCAG). But don’t take our word for it — check...
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What's New in CRISPR - May 2019
TypeBlog Post...library (~60,000 gRNAs) that targets ~4,000 E. coli genes with ~15 gRNAs per gene and includes ~10,000 gRNAs...APOBEC1 causes genome-wide deamination of RNA cytosines in human cells. Thus, they engineered two CBE ... -
How Do I Place an Addgene Order Using a PO Number?
TypeBlog Post...online from your favorite store is easy. Ordering genes on plasmids online is a little trickier -- but at...to generate the legal agreement (MTA), which streamlines the process and saves you time. Anyone affiliated... -
6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results
TypeBlog Post...the DNA sequencing reaction should be repeated. Guidelines to help with DNA sequencing troubleshooting and...Most chromatogram viewing programs (even the free ones) allow you to edit the sequence. I hope these... -
Microbes: Look, Listen, and Tell
TypeBlog Post...ultrasound to image bacteria in vivo: acoustic reporter genes’ on our blog. Creative approaches to presenting...Bourdeau, Raymond W., et al. "Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian... -
What's New in CRISPR - Spring 2019
TypeBlog Post...reaction. STAgR is highly customizable as vector backbones, and gRNA structure, numbers, and promoters can...marker excision method for marking distinct cell clones developed by the Michalis Averof lab. It’s an alternative... -
Developing Transferable Skills During Science Training
TypeBlog Post...involving multiple scientists, create and stick to timelines Be a mentor in a formal program Start something... publications like FierceBiotech to “learn the business” Attend events with local networking and trade... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog Post...2006, we saw an increase in popularity of empty backbones for shRNA expression, such as pLKO.1 puro and ...shRNA for use as a negative control, and empty backbones such as pBABE-puro for retroviral expression and... -
What's New in CRISPR - December 2019
TypeBlog Post...Yachie’s lab recently developed a base editor that combines the abilities of C-to-T and A-to-G base editors...TadA) to a catalytically impaired Streptococcus pyogenes Cas9. They called this editor Target-ACE (adenine... -
Seeing Red: Simple GFP Photoconversion
TypeBlog Post... dynamics can be undertaken using available GFP lines. Like many great scientific discoveries, this one...for microscopy enthusiasts! Addgene has empty backbones containing GFP variants used by Sattarzadeh et... -
Three Tips to Organize Your Lab Notebooks in the New Year
TypeBlog Post...you experiments, keep track of upcoming grant deadlines, and sync everything to your calendar. Ditch the...Tasks to create lists of your upcoming tasks, set deadlines, and arrange automatic email reminders. These ... -
Scientific Sharing in the Time of COVID-19: Databases and Resources
TypeBlog Post...involved with COVID-19 to researchers in other disciplines (bioinformatics, image analysis, AI…). How to...new Center for COVID-19 Response and Pandemic Preparedness (CCRP2) at Rutgers University not only supports... -
2023 and 2024: Reflections and Looking Ahead
TypeBlog Post...future. Over the years, we have benefited from the business rigor and efficiencies typically adopted by for-profit... Chonnettia Jones... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog Post...and KD were shown to be functional in cultured Chinese hamster ovary (CHO) cells, as well as in cortical...activity regulation. Heidmann D, Lehner CF. Dev Genes Evol. 2001 Sep;211(8-9):458-65. doi:10.1007/s004270100167... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog Post...Public library. Being in the presence of scientific heroines is always inspiring. I was especially excited ...been able to use CRISPR/Cas9 to generate mice with genes knocked out in the insulin signaling cascades in... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post...site. In the SPARC system, PhiC31 irreversibly recombines one of the two competing attP sites to drive ...read-through transcripts of the effector gene. Effector genes, any gene that you want to control the expression... -
Plasmids 101: What is a plasmid?
TypeBlog Post...gene. Vital component for expression vectors: determines which cell types the gene is expressed in and... flexibility, versatility, safety, and cost-effectiveness enable molecular biologists to broadly utilize... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog Post... Rare Disorders (NORD). NORD raises money and awareness for rare disease and helps patients find the correct...an organization dedicated to raising funds and awareness of FOP. Please consider running with us or making... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog Post...raffled off one of our highly sought-after "nice genes…" tshirts, and the lucky winner was Shagun Sharma...to be had at ASM Microbe as well as the serious business of science. Two symposia on Sunday afternoon were... -
Management for Scientists: Managing vs. Leading
TypeBlog Post...any goal or finishing any project. Doers are the ones who do the work Doers focus on understanding what...Marshall Goldsmith, with Mark Reiter Harvard Business Review 10 Must Reads: On Leadership (Articles ...