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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 939 results for: OMP

Showing: 601 - 620 of 939 results

  1. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...is guided by an RNA molecule, the gRNA, to a complementary site in the genome where it will proceed to ...that Cas14 also indiscriminately cleaves non-complementary ssDNA when engaged by its target sequence. Why...Cas7-11 Cas7-11 is a naturally occurring RNase composed of the fusion of several Cas7 and Cas11 domains...

  2. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...validated their platform on clinical stool samples by comparison to qPCR and demonstrated that it can also detect...without fixation or preparation, and deliver CRISPR components for gene disruption or CRISPRi. While designed...bacteriophage shell protein or a bacterial microcompartment protein. The expression of this fusion is ...

  3. RNA Extraction Without A Kit

    Type
    Blog Post
    ...or tissue collection method, so biotechnology companies have marketed several products that help to further... RNAzol®, QIAzol®  and sold by many different companies). TRIzol® is an all-in-one acid-guanidinium-phenol... is the carryover of DNA that can potentially complicate results of a downstream application such as quantitative...

  4. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ... was also between 1013 - 1014 vg/kg, a dosage comparable to those currently used in human gene therapy...fluorescent voltage indicators, however, fall short compared to GFP-based sensors in terms of response and ...precision of gene expression and reduces toxicity as compared to previous Cre/lox or drug-induced Cre systems...

  5. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ... light sensitive and have enhanced conductance compared to current channelrhodopsins. Their new channelrhodopsins...neurons do not express as many copies of the ChRs as compared to direct injection. This lower multiplicity of...better suited for systemic delivery with PHP.eB compared to the existing ChR2(H134R).  In the second experiment...

  6. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ... had been collecting data and documenting the compliance of universities with the FLSA in our online resource... salary raising as the only viable option for compliance. However, 10 days prior to the implementation...opportunity of the lapsed mandate (or are being compelled at the state level, in the case of some public...

  7. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...Stanford University. Sex and gender are critical components of biological research that are often forgotten...cost at this early stage of research is minimal compared to that of human clinical trials. And, as Janine...studies that draw from a body of research that is incomplete (because sex was not analyzed) may harm humans...

  8. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...Cloner, and Snapgene), and save these to a shared computer or server. This will ensure that future members...more than two sequencing reactions. In the more complex cases, we sometimes have to design new primers ...saves us the step of purifying the DNA and gives comparable success rates and sequence quality, which is ...

  9. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...difficult to functionally manipulate and study in comparison to protein-coding genes (Rinn and Chang 2012)...deletions and insertions in the DNA sequence can completely disrupt expression of the transcribed ncRNA (...interactions between ncRNAs and adjacent genes are complex and not yet fully understood, so it is important...

  10. CRISPR in the Clinic

    Type
    Blog Post
    ...well as the introduction of the cancer-fighting component, CAR. Clinical trials are underway to explore ... have involved turning on fetal hemoglobin to compensate for defects in the adult hemoglobin produced ...for use in the next few months. Several other companies have followed suite, with Beam Therapeutics initiating...

  11. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...choosing antibodies   Complexity is a key factor Biological systems tend to be complex with a variety of ...proteins interacting. Experiments tend to be equally complex with users trying to probe multiple targets in ...

  12. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...itself. Among other things, science communication comprises broadcasting, science writing, and certain aspects... get on stage for a Science Slam – a sort of competitive TED talk – to win the audience’s applause with...photography and participate in a science photo competition. Or nominate yourself as an educator for a short...

  13. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...get the molecular components of CRISPR/Cas9 (Cas9 protein and the crRNA-tracrRNA complex) and the donor ...destabilizes the cell’s outer membrane and allows the components to physically enter the cells so they can go ...

  14. Engaging with science and society at pgEd

    Type
    Blog Post
    ...of science, even while I was a bench scientist completing my graduate studies in molecular biology and ...for the past three years. pgEd is a small team comprised of individuals from diverse backgrounds and disciplines...social context. This “general public” audience comprises the taxpayers who fund much of scientific research...

  15. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...response against AdV. Importantly, recombination-competent adenovirus (RCA) can be generated if the E1 gene...negligible toxicity. However, their production is more complex and the presence of helper virus contaminants have...amplification.*Pro-Tip*: The cells will become completely confluent and the media will turn yellow. Do ...

  17. Lab Automation at Addgene

    Type
    Blog Post
    ...allowing us to do up to 96 transformations in one run compared to doing about 50 transformations in the same ...increased plasmid demand it was too time consuming to complete this process by hand and we welcomed yet another...liquid handler can fill 400 vials in 35 minutes compared to a lab assisstant filling about 200 vials in...

  18. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...machinery.   Well, I’ve got some good news: complete DNA demethylation can be achieved by dCas9 alone...too.  High dCas9 and gRNA expression are key for complete DNA demethylation – tight binding is crucial for...Charlton J, Jung EJ, Mattei AL et al. (2020) TETs compete with DNMT3 activity in pluripotent cells at thousands...

  20. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...structure of the hairpin-DNA complex is thought to destabilize the transcription complex, initiating cleavage ...mechanism, whereas Polymerase II termination is more complex and involves two RNA polymerase-associated proteins...
Showing: 601 - 620 of 939 results