We narrowed to 761 results for: Rest

1. Fluorescent Biosensors for Measuring Autophagic Flux

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   Blog Post

   ...mitophagy. Rosella can also be fused to a protein of interest to track the autophagy of that protein. Find ...

2. Addgene's Top 10 Blog Posts of 2015

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   Blog Post

   ...strains you can use to express your protein of interest. 4. Plasmids 101: Blue-White Screening Getting...

3. What is Polymerase Chain Reaction (PCR)

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   Protocol

   ... You may also like... Plasmid Cloning by PCR Restriction Digest of Plasmid DNA DNA Ligation Background... DNA: Contains the portion of DNA that we are interested in amplifying for analysis and manipulation. ...

4. Trading Spaces - Visiting Scientist Edition

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   Blog Post

   ...If you’re interested in learning a new skill, getting access to equipment you don’t have, or maybe both...

5. DAP arrays for multiplex gene editing

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   Blog Post

   ...editing, in virtually any cell type or organisms of interest with only minimal genetic payloads. Different ...

6. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

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   Blog Post

   ... is opened and equilibrium with ambient air is restored. We have no evidence to suggest that the transient...

7. Learning Technical Skills at a Lab Central Workshop

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   Blog Post

   ...Komuhendo is a Lab Technician at Addgene who is interested in medicine and music.       Additional Resources...

8. Cultivating Community Science at BosLab

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   Blog Post

   ...realized that it was easy to find people who were interested. For example, our journal club discussion on ...

9. Plasmids 101: NGS Quality Control for Pooled Libraries

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   Blog Post

   ...and if you have any tips, input, or stories of interest about library use, let us know in the comments...

10. Improving Transduction Efficiency with Exosome AAVs

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    Blog Post

    ...vehicle for drug delivery that has recently gained interest special contexts, such as the delivery of therapeutic...

11. Addgene Welcomes John Doench to the Advisory Board

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    Blog Post

    ...drawing me towards the Broad. In order to ask interesting biological questions, you need a tremendous amount...

12. Resume Writing for Non-academic Science Careers

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    Blog Post

    ...browsing job postings and look at the jobs you are interested in. Consider listing the skills you already have...

13. Plasmids 101: Mammalian Vectors

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    Blog Post

    ...assayed conveniently. A direct test for your gene of interest may be another method to assess transfection success...

14. Virus Protocol - Generating Stable Cell Lines

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    Protocol

    ...generate stable cell lines expressing a gene of interest from an integrated lentiviral vector....generate stable cell lines expressing a gene of interest from an integrated lentiviral vector. Unlike the...

15. Pouring LB Agar Plates

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    Protocol

    ...your own LB/agar plates with your antibiotic of interest. Last Upload: September 14, 2016 Protocol Video...selecting only bacteria containing the plasmids of interest (for example, fewer satellite colonies will grow...

16. Fluorescent Proteins 101: Luciferases

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    Blog Post

    ...thousands of reporter constructs with a gene of interest already inserted. In addition, we have recently...

17. AAV Q&A with Tim Miles

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    Blog Post

    ...understanding that AAV-BR1 has the same genome size restrictions as other AAV. Therefore, one should aim to keep...

18. Sweating the Small Stuff: Details in the Lab

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    Blog Post

    ...experiment.  For example, I used to constantly underestimate time needed to prep for an experiment (labeling...

19. Antibodies 101: Buffers, Storage, and Conjugates

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    Blog Post

    ...protein in an experiment with many proteins of interest! Research the brightness of your fluorophores ...

20. Antibodies 101: Isotypes

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    Blog Post

    ...primary antibody but doesn’t bind your antigen of interest. Isotype controls are used to make sure the staining...