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... is opened and equilibrium with ambient air is restored. We have no evidence to suggest that the transient...

...If you’re interested in learning a new skill, getting access to equipment you don’t have, or maybe both...

...editing, in virtually any cell type or organisms of interest with only minimal genetic payloads. Different ...

...vehicle for drug delivery that has recently gained interest special contexts, such as the delivery of therapeutic...

...Komuhendo is a Lab Technician at Addgene who is interested in medicine and music.       Additional Resources...

...drawing me towards the Broad. In order to ask interesting biological questions, you need a tremendous amount...

...browsing job postings and look at the jobs you are interested in. Consider listing the skills you already have...

...assayed conveniently. A direct test for your gene of interest may be another method to assess transfection success...

...transfect mammalian cells with your plasmid of interest....Transfections allow for transient expression of a gene of interest in a target cell line and can be useful for short...

...and photobleaching. Researchers have long been interested in using luminescence to get around these issues...

...Variants Identifies Oncogenic Alleles. Kim E, Ilic N, Shrestha Y. et al. Cancer Discovery 2016. PubMed PMID: ...

...multi-gene editing with a single crRNA array. Are you interested in using Cpf1 multiplexing in your research? ...

... developments on this project and on all the interesting things going on at Addgene. Sharing speeds science...

...mRNAs and two nuclear long non-coding RNAs. One interesting twist to Cas13d enzymes is their cleavage pattern...

...These modifications result in conformationally restricted RNA which improves mismatch discrimination and...

...understanding that AAV-BR1 has the same genome size restrictions as other AAV. Therefore, one should aim to keep...

...experiment.  For example, I used to constantly underestimate time needed to prep for an experiment (labeling...

...protein in an experiment with many proteins of interest! Research the brightness of your fluorophores ...

...primary antibody but doesn’t bind your antigen of interest. Isotype controls are used to make sure the staining...

... binds to a specific region of your protein of interest. For example, if you are studying a protein that...