Addgene

We narrowed to 776 results for: SCR

Showing: 601 - 620 of 776 results

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CRISPR-mediated Plant Base Editors

Type

Blog Post

...into E. coli harboring the defective CamR gene. Screening for surviving colonies and subsequent rounds of...
Twenty Years of Sharing: Addgene's Viral Vector Service

Type

Blog Post

...development. We’ve shared what we’ve learned, through manuscripts (Ersing et al., 2023; Haery et al., 2019; McDade...
Overcoming the AAV Size Limitation for CRISPR Delivery

Type

Blog Post

...as potent and specific? The two AAV strategies described above showed successful target modification, indicating...
Antibodies 101: Immunoprecipitation

Type

Blog Post

...primary antibody and serum immunoglobulins indiscriminately, causing competition for binding sites. In...
CRISPR 101: Any Base Transversion Editors

Type

Blog Post

... able to edit A → Y (Y = T or C) in 67.17% of screening cells (Tong, Wang, et al., 2023). Based on this...
The Challenges of Cell Culture

Type

Blog Post

...free of contamination and match their original description. The oldest, largest, and most diverse of these...
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

Type

Blog Post

... live cells in real time. Previously, we have described a number of practical considerations that should...
Important Considerations in Optogenetics Behavioral Experiments

Type

Blog Post

...of mouse transgenics. Everything that has been described is intended for the mouse; however, all the steps...
Tips for Using FRET in Your Experiments

Type

Blog Post

...gene of interest: Plasmid Color Expression Description pPROEX Aqua Cyan Bacterial Expresses Aquamarine...
Fluorescent Proteins 101: When GFP lets you down

Type

Blog Post

...probes are based on cofactor binding proteins as described above. The smallest tags are peptides that are...
March for Science

Type

Blog Post

...freeze all grants, contracts The Guardian: Trump to scrap Nasa climate research in crackdown on “politicized...
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Type

Blog Post

...C-terminus of the fluorescent protein. I prefer to describe the different parts of the fusion from N- to C-terminus...
Plasmids 101: Biotinylation

Type

Blog Post

...biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice...
Antibodies 101: Beyond Surface Labeling

Type

Blog Post

...the same). Examples of such targets include transcription factors, histones, and DNA repair enzymes. Note...
An Integrin Antibody Toolkit from IPI

Type

Blog Post

...collection includes 26 unique antibodies that bind discrete subunits or particular subunit pairs. Some of ...
Tips for Getting a Faculty Position

Type

Blog Post

...feedback. Many of these documents are too vaguely described in other sources of guidance or the document examples...
Five Popular Model Organisms, Part 2

Type

Blog Post

...Xing, X.-H. (2018). Pooled CRISPR interference screening enables genome-scale functional genomics study...
New Optogenetic Tools for Cytoskeleton and Membrane Control

Type

Blog Post

...plasma membrane. In previous publications, they described tools to control formation of lamellipodia and...
10 Steps to a Perfect Science Talk

Type

Blog Post

...listeners to be thinking “What happens next?” Describe how you pushed to a solution or overcame obstacles...
Thoughts on the Future of Research 2015 Boston Symposium

Type

Blog Post

...PI on the panel even suggested projecting your manuscript onto a wall and reading it out loud. While it...

Showing: 601 - 620 of 776 results

Results per page:

CRISPR-mediated Plant Base Editors

Twenty Years of Sharing: Addgene's Viral Vector Service

Overcoming the AAV Size Limitation for CRISPR Delivery

Antibodies 101: Immunoprecipitation

CRISPR 101: Any Base Transversion Editors

The Challenges of Cell Culture

Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

Important Considerations in Optogenetics Behavioral Experiments

Tips for Using FRET in Your Experiments

Fluorescent Proteins 101: When GFP lets you down

March for Science

Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Plasmids 101: Biotinylation

Antibodies 101: Beyond Surface Labeling

An Integrin Antibody Toolkit from IPI

Tips for Getting a Faculty Position

Five Popular Model Organisms, Part 2

New Optogenetic Tools for Cytoskeleton and Membrane Control

10 Steps to a Perfect Science Talk

Thoughts on the Future of Research 2015 Boston Symposium

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