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We narrowed to 1,052 results for: STI

Showing: 601 - 620 of 1052 results
  1. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...Restriction digest screening for ITR deletion is still necessary. To save time and money, I like to miniprep...Production protocol, I take a lazier approach for harvesting my AAV. I skip the PEG precipitation of the HEK293...PEG solution. PEG takes several hours on a heated stir plate to dissolve and requires monitoring so that...autoclaving, which also takes time. Rather than investing all of this effort in preparing reagents, I choose...
  2. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...impressive, full-color images of proteins. But they are still flat. To truly appreciate the 3D structure of these...models function as thinking tools – eliciting questions like “what does this model representt?" ... "how...creating physical models of proteins that are being investigated in their labs. Soon after we began working with...of the night dreaming about the conformational gymnastics that this protein must undergo as it interrogates...
  3. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ... vector (deposited by David Root of the Broad Institute) has consistently been one of the repository’s...have used the protocol for this plasmid, and if it still being used. After learning of it's continued popularity...is the creation of ZappyLabs. Currently in beta testing, the software aims to be a life science protocol...bring your phone in the lab, though, honestly, this still grosses me out. Maybe it’s just me – I didn’t get...
  4. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ... the “Align two or more sequences” option to drastically reduce waiting time for search results. Optimizing...with the "Align two or more sequences" option and pasting your expected protein sequence into the Subject...webservices/#pairwise_sequence_alignment_psa Happy BLASTing! Do you have any tips for using BLAST to confirm...plasmid sequencing results or comments on our suggestions? Share your thoughts here to help other labs ...
  5. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...2020). Harvest and purification At its core, harvesting recombinant antibodies consists of separating...Created with biorender.com   Another method of harvesting uses magnetic beads. In this method, beads are... the efficacy of our antibodies through stress testing. An aliquot of our recombinant antibodies is stored...assays showed that the majority of antibodies are still equally effective compared to those aliquots frozen...
  6. CRISPR 101: RNA Editing with Cas13

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    Blog Post
    ...editor, the lab started with the Cas13 scaffold, testing a whopping 21 Cas13a orthologs, 15 Cas13b orthologs... the plasmids for the RESCUE RNA editors here! Testing and improving RNA editing from the Cas13b REPAIR...knockdown is more precise than shRNA knockdown, it still displays substantial off-target activity. By mutating...Addgene. East-Seletsky, Alexandra, et al. "Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA...
  7. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...is currently investigating the molecular biology of synaptic transmission and plasticity.     Javier García-Nafría...co-transformation with linearized vector backbone. DpnI digestion - 1 μl DpnI is added to the PCR mix to remove ... García-Nafría is an investigator scientist at the MRC Laboratory of Molecular Biology. He is interested...
  8. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...-wide screens to effectively and efficiently investigate gene function. CRISPR libraries can be used to...labs of David Root and John Doench at the Broad Institute have developed human genome-wide CRISPRko (knock...lowly expressed genes or genes that are more drastically affected by gene activation rather than depletion...resistance in A375 cells. The SAM system (CRISPR/Cas9 Synergistic Activation Mediator) uses an improved activator...
  9. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...Janelia Research Campus, Howard Hughes Medical Institute. Chemistry is dead, long live chemistry! The discovery.... These impressive advances lead to an obvious question: In this age of GFP and its ilk, why should cell...Janelia Research Campus, Howard Hughes Medical Institute. He would rather be in the lab making new fluorophores...PubMed PMID: 26575238. 5. Crivat, Georgeta, and Justin W. Taraska. "Imaging proteins inside cells with...
  10. CRISPR 101: Making a Knock-In Cell Line

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    Blog Post
    .... To kick off a knock-in experiment the first questions are where, which, and what. Identify where in ...like to introduce the edit. Finally, you must stipulate what sequence to introduce, which is done through...HDR edit, you can try one or more of the below suggestions. Disable the other repair mechanisms Mammalian...! Cas12a is a Cas family member which generates sticky ends upon cutting. Cas12a may promote HDR by generating...
  11. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...clicking the ‘Search’ button a new content-aware suggestion dropdown list will let you jump directly to the...types of searches including depositing labs and institutions. For example, search for “Melina Fan” and you... in Parkinson’s disease. Knowing the primary investigator where the plasmid was created can narrow your...plasmids specifically designed for this purpose: Still stumped? Ask an Addgenie The newly launched search...
  12. Technical Design of a Western Blot

    Type
    Blog Post
    ... It can be so overwhelming that you might just stick with the protocol your labmate handed you — after... contain any denaturing agents. However, you’ll still have to lyse your proteins. For this post, we are...stronger reducing agent than BME and has a less… distinctive smell. BME smells like rotting eggs but has a...However, these systems are expensive, generate plastic waste, and limit the amount of optimization that... may only have access to one or another. Most institutes have moved towards CCD cameras over the past ...
  13. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...interneurons across species and will undoubtedly constitute a valuable tool for the research community. You...CaMKII and RhoA during induction of structural plasticity in single dendritic spines. The set of CyRFP ...variants to develop five new base editors with distinct PAM sequences, expanding the number of available...xylose-inducible dCas9 paired with a single, unique, and constitutively expressed sgRNA targeting the 5’ end of each...to premature aging and cancer. The Denchi lab investigated an aspect of telomere length regulation called...
  14. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...with a different editing window than existing CGBEs. This distinct approach means researchers can now select...the adenine transversion editor AYBEv3. This tool still retained considerable A → G editing activity at ...rates ranging from 4.7% to 30% at tested sites. Still, it opened a new avenue for base editor development...
  15. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...Understanding the characteristics of natural plasmids and how they function in nature can inform us on...other hosts. For example, the pJD4 plasmid harbors distinct origins (ori1, ori2, ori3) and replication initiation...these plasmids are compatible with.  Table 1: Non-exhaustive list of common broad host range plasmids and ... P. C., & Wong, H. L. (2023). Development of constitutive and IPTG-inducible integron promoter-based expression...
  16. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ... delve deeper and look specifically at the characteristics and capabilities an ELN needs to facilitate...Goettingen, and several other large research institutions. The following graphic depicts the ways in which...banks and sequencing files is likely stored in institutional and lab servers specifically designed to hold...illustrated in this video, and to data stored on institutional and lab servers. Connectivity to other tools...
  17. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ... PMC5726555. 4. Xie, Kabin, Bastian Minkenberg, and Yinong Yang. "Boosting CRISPR/Cas9 multiplex editing... a single base. If you heard about CRISPR, one question probably comes to mind: can CRISPR be applied ...Ag-biotechnologist in the National Rice Research Institute, Cuttack, Odisha, India. Currently Kutubuddin ...
  18. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...there are still many more features to consider when designing a full gene. A non-exhaustive list includes...be many possible ways to encode them and what constitutes the “appropriate” codon is not necessarily obvious...PubMed Central PMCID: PMC2970903. 6. Pechmann, Sebastian, and Judith Frydman. "Evolutionary conservation...
Showing: 601 - 620 of 1052 results