We narrowed to 712 results for: STO

1. Addgene's Cancer Collection Pages Connect the Research Community

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   Blog Post

   ...be to follow up on these promising results and customize the small molecules for a higher affinity fit....

2. Google Forums Round Up: First Impressions of NgAgo

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   Blog Post

   ...in AT-rich regions of the genome or just after a stop codon (as required for C-terminus epitope tagging...

3. Experimenting with New Careers while in Grad School

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   Blog Post

   ...my blog helped immensely when CINSO started brainstorming the resources we wanted to provide online. ...

4. Tips for Using BLAST to Verify Plasmids

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   Blog Post

   ... BLAST options and for our suggested blastx customization. Similar to nucleotide sequences, proteins often...

5. Simplify Cloning with in vivo Assembly

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   ...containing buffer, polymerase and dNTPs, can be stored frozen: just defrost and add your primers and template...

6. DNA Purification Without a Kit

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   Blog Post

   ...Before you reach for that silica spin column, stop to consider some ways to purify DNA without a kit....

7. A Guide to Starting Your Own Journal Club

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   Blog Post

   ...you find yourself in this situation, considering stopping (temporarily or permanently) the recurring meeting...

8. Course-Based Undergraduate Research Experiences (CUREs)

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   Blog Post

   ...becoming just the fifth repeat Nobel Prize winner in history. Interestingly, he also adds to the disproportionately...

9. CRISPR 101: Making a Knock-In Cell Line

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   Blog Post

   ...change your protein of interest.   Fig. 2: Stop re-cutting with PAM and guide RNA disrupting mutations...

10. Negative Can Be Positive: Open AAV Data with Addgene

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    Blog Post

    ...complex biological reagents; many factors, like storage buffers and conditions (3,5,8), injection titers...

11. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    Blog Post

    ....1709035114 Rubin BE, Diamond S, Cress BF, Crits-Christoph A,  He C , Xu M, Zhou Z, Smock DC, Tang K, Owens...

12. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    Blog Post

    ...in large enough quantities (and the correct stoichiometric ratios) to generate useable data. What’s more...

13. CRISPR 101: RNA Editing with Cas13

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    Blog Post

    ...known pathogenic alleles, or introduce a premature stop codon to render an RNA nonfunctional. In their quest...

14. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    Blog Post

    ... 2020. We fit our model for each region using stochastic gradient descent in Pytorch, which gave us the...

15. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    Blog Post

    ...encodes Cas9 and contains a site for inserting a custom gRNA.  While this methodology has proven to be ...

16. MXS Chaining

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    Blog Post

    ...Subcellular localization 1 TagBFP 399nm/ 456nm histone 2B (H2B) Chromatin 2 Cerulean 433nm/ 475nm Lyn-tag...

17. Lentiviral Vector Uses and Overview

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    Blog Post

    ...applications of lentiviral vectors! Lentiviral vector history and components In the early 1990s, researchers ...

18. Plasmids 101: Colony PCR

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    Blog Post

    ...cultures of your positive clones. If you want to store your clones longer term, just streak the colonies...

19. CRISPR Antimicrobials

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    Blog Post

    ...targeting a process essential to most bacteria, custom CRISPR antimicrobials can target specific sequences...

20. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    Blog Post

    ...of Dr. Elke Mühlberger and Dr. Rachel Fearns at Boston University School of Medicine, Department of Microbiology...