We narrowed to 729 results for: STO

1. Tips for Titering Your Lentiviral Preps

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   Blog Post

   ...significantly higher than the same prep that has been stored in the freezer. If you plan to freeze a batch of...

2. Common Injection Routes in Mice

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   Blog Post

   ...experimental therapies or pain relief, straight to the stomach. This technique involves administering medication...

3. Magnetic Control of Proteins: More than a Dream

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   Blog Post

   ...MagLOV. Andrew was thrilled to see that MagLOV’s “astonishing” magnetoresponse was clearly visible whether ...

4. Tips to Make the Most of a Scientific Conference

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   Blog Post

   ...research, gathered up your data into an exciting story, and are ready to present your findings at a conference...

5. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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   Blog Post

   ...patterns in chromatin interactions. The chromatin story told by CAPTURE 2.0 is important, but it’s not the...

6. Viral Vectors 101: Voltage Indicators

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   Blog Post

   ...a genetic component. The VSD components have historically come from a variety of sources – voltage-gated...

7. AAV Vector Quality Control: Going the Extra Mile with NGS

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   Blog Post

   ...addition to genome identification, we developed custom open-access Python scripts that can perform a deeper...

8. Addgene's Cancer Collection Pages Connect the Research Community

   Type

   Blog Post

   ...be to follow up on these promising results and customize the small molecules for a higher affinity fit....

9. Google Forums Round Up: First Impressions of NgAgo

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   Blog Post

   ...in AT-rich regions of the genome or just after a stop codon (as required for C-terminus epitope tagging...

10. Experimenting with New Careers while in Grad School

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    Blog Post

    ...my blog helped immensely when CINSO started brainstorming the resources we wanted to provide online. ...

11. Tips for Using BLAST to Verify Plasmids

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    Blog Post

    ... BLAST options and for our suggested blastx customization. Similar to nucleotide sequences, proteins often...

12. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    Blog Post

    ....1709035114 Rubin BE, Diamond S, Cress BF, Crits-Christoph A,  He C , Xu M, Zhou Z, Smock DC, Tang K, Owens...

13. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    Blog Post

    ...in large enough quantities (and the correct stoichiometric ratios) to generate useable data. What’s more...

14. CRISPR 101: RNA Editing with Cas13

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    Blog Post

    ...known pathogenic alleles, or introduce a premature stop codon to render an RNA nonfunctional. In their quest...

15. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    Blog Post

    ... 2020. We fit our model for each region using stochastic gradient descent in Pytorch, which gave us the...

16. Negative Can Be Positive: Open AAV Data with Addgene

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    Blog Post

    ...complex biological reagents; many factors, like storage buffers and conditions (3,5,8), injection titers...

17. Simplify Cloning with in vivo Assembly

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    Blog Post

    ...containing buffer, polymerase and dNTPs, can be stored frozen: just defrost and add your primers and template...

18. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    Blog Post

    ...encodes Cas9 and contains a site for inserting a custom gRNA.  While this methodology has proven to be ...

19. DNA Purification Without a Kit

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    Blog Post

    ...Before you reach for that silica spin column, stop to consider some ways to purify DNA without a kit....

20. A Guide to Starting Your Own Journal Club

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    Blog Post

    ...you find yourself in this situation, considering stopping (temporarily or permanently) the recurring meeting...