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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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  4. Cloning

    Type
    Guide
    ...fuse. A major disadvantage is that you need to be set up to grow, transform and purify DNA from yeast. ...promoters and into backbones with different selection cassettes). Although gateway cloning is a simple and efficient...

  6. General Transfection

    Type
    Protocol
    ... transfected using 1:1, 1:2, 1:3 and 1:6 µg of pRosetta :µg of PEI. The 1:2 and 1:3 ratios provided high...

  7. Lentivirus Production

    Type
    Protocol
    ...were transfected with the GFP-expression plasmid pRosetta using μg total DNA to μg PEI ratios of 1:1, 1:...

  8. Colony Formation Titering Assay

    Type
    Protocol
    ...indicated serial dilutions of the lentiviral vector pRosetta . The following day, cells were treated with puromycin...

  10. Plan Your Experiment

    Type
    Guide
    ... you started using CRISPR in your research and setting up CRISPR experiments. We will focus on making ...DNA to serve as template for the repair. Once you settle on your gRNA, you can design a donor DNA to have...variety of modifications and will likely contain a subset of wild type cells. If you are screening a variety...

  11. Antibody Guide

    Type
    Guide
    ...non-biological replicates) often each require their own set of controls. Multiplex Assays Flow cytometry, IF,... cytometry requires a negative control, a small subset of the sample with no antibodies. It also requires...requires single-antibody controls, a small subset of the sample labeled with only one antibody, for each ...

  12. Adenovirus Guide

    Type
    Guide
    ...DNA. However, if the plasmid contains a Gateway cassette containing the ccdB gene, a ccdB-resistant strain...protein-coding genes and complex gene expression cassettes are often too big to be packaged in rAAV vectors...enough to carry the Cas9 gene and a gRNA expression cassette in one single viral particle. Vaccine Development...

  13. Sequencing Primers

    Type
    Guide
    ... of Gateway cassette, forward primer GW-5' AATCTCGCCGGATCCTAACT 5' end of Gateway cassette, reverse primer...

  14. CRISPR Guide

    Type
    Guide
    ..., D., Romanel, A., Demichelis, F., Inga, A., & Cereseto, A. (2018). A highly specific SpCas9 variant is...Protocols in Molecular Biology . 115 . PMID: 27366891 Rousset, F., Cui, L., Siouve, E., Becavin, C., Depardieu... M. W., Yamano, T., Nishimasu, H., Nureki, O., Crosetto, N., & Zhang, F. (2017). Engineered Cpf1 variants...

  15. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...regions between the two ITRs with a gene expression cassette that typically contains the transgene of interest...DNA. However, if the plasmid contains a Gateway cassette containing the ccdB gene, a ccdB-resistant strain...

  16. Chemogenetics Guide

    Type
    Guide
    ...and inhibiting DREADDs in the same experimental setup) were not possible. To overcome this limitation,...

  17. Optogenetics Guide

    Type
    Guide
    ...below, can be targeted and expressed in specific subsets of neurons, allowing precise spatiotemporal control...
Showing: 601 - 617 of 617 results