We narrowed to 618 results for: Set

1. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

   Type

   Blog Post

   ...course of infection, when HIV-1 is limited to a subset of T-cells, but it is unclear if enough T-cells...

2. Thoughts on the Future of Research 2015 Boston Symposium

   Type

   Blog Post

   ...training in the academic research environment is setup to prepare trainees for further work in academia...

3. Viral Vectors 101: Optogenetic Tools

   Type

   Blog Post

   ...Figure 1: Example of an experimental optogenetic setup. A light source delivers light pulses to the subject...

4. Viral Vectors 101: AAV Variables That Matter

   Type

   Blog Post

   ...adeno-associated viral vector serotypes 1, 2, 5, 8 and 9 in marmoset, mouse and macaque cerebral cortex. Neuroscience...

5. Antibodies 101: Antibody Engineering and Directed Evolution

   Type

   Blog Post

   ...; font-weight: normal; text-decoration: none","font_set":"DEFAULT"},"button_hover_color":{"color":"#0052a9...

6. Live and Let Dye: Self-Labeling Protein Tags

   Type

   Blog Post

   ...emission depletion (STED) nanoscopy. Scale bar, 2 μm. Inset shows comparison with standard confocal microscopy...

7. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

   Type

   Blog Post

   ...Imaging of the cross-presenting dendritic cell subsets in the skin-draining lymph node." Proceedings of...

8. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

   Type

   Blog Post

   ...after completing her postdoctoral training from Massachusetts General Hospital and Harvard Medical School....

9. General Transfection

   Type

   Protocol

   ... transfected using 1:1, 1:2, 1:3 and 1:6 µg of pRosetta :µg of PEI. The 1:2 and 1:3 ratios provided high...

10. Lentivirus Production

    Type

    Protocol

    ...were transfected with the GFP-expression plasmid pRosetta using μg total DNA to μg PEI ratios of 1:1, 1:...

11. Colony Formation Titering Assay

    Type

    Protocol

    ...indicated serial dilutions of the lentiviral vector pRosetta . The following day, cells were treated with puromycin...

12. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type

    Protocol

    ...especially in the corner of the well as cells tend to settle there. Once the cells have expanded but before ...

13. Adenovirus Guide

    Type

    Guide

    ...DNA. However, if the plasmid contains a Gateway cassette containing the ccdB gene, a ccdB-resistant strain...protein-coding genes and complex gene expression cassettes are often too big to be packaged in rAAV vectors...enough to carry the Cas9 gene and a gRNA expression cassette in one single viral particle. Vaccine Development...

14. Sequencing Primers

    Type

    Guide

    ... end of Gateway cassette Forward GW-5' AATCTCGCCGGATCCTAACT 5' end of Gateway cassette Reverse H1 TCGCTATGTGTTCTGGGAAA...

15. CRISPR Guide

    Type

    Guide

    ..., D., Romanel, A., Demichelis, F., Inga, A., & Cereseto, A. (2018). A highly specific SpCas9 variant is...Protocols in Molecular Biology . 115 . PMID: 27366891 Rousset, F., Cui, L., Siouve, E., Becavin, C., Depardieu... M. W., Yamano, T., Nishimasu, H., Nureki, O., Crosetto, N., & Zhang, F. (2017). Engineered Cpf1 variants...

16. Adeno-associated virus (AAV) Guide

    Type

    Guide

    ...regions between the two ITRs with a gene expression cassette that typically contains the transgene of interest...DNA. However, if the plasmid contains a Gateway cassette containing the ccdB gene, a ccdB-resistant strain...

17. Chemogenetics Guide

    Type

    Guide

    ...and inhibiting DREADDs in the same experimental setup) were not possible. To overcome this limitation,...

18. Optogenetics Guide

    Type

    Guide

    ...below, can be targeted and expressed in specific subsets of neurons, allowing precise spatiotemporal control...