We narrowed to 740 results for: Sis;

1. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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   ...2008) Pseudotyped Vesicular Stomatitis Virus for Analysis of Virus Entry Mediated by SARS Coronavirus Spike...

2. From Lab Bench to Leadership: Transitioning into Management as a PhD

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   ...burdensome task of explaining the steps to a lab assistant. However, I recognized the need to shift my mindset...

3. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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   ...polypurine tract; recognition site for proviral DNA synthesis. Increases transduction efficiency and transgene...

4. Why and how to start studying chromatin (with Addgene's help)

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   ...chromatin-related plasmid in a collection that grows on a daily basis, the lists below provide a snapshot of relevant ...

5. Plasmids 101: Inducible Promoters

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   ...present, YFI is inactive, preventing repressor cI synthesis and allowing reporter gene transcription to take...

6. How Addgene Creates Content

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   ...reviews on blog posts are done on a case-by-case basis, as many of our blog contributors primarily write...

7. Plasmids 101: Screens vs. Selections

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   ...trying to identify genetic conditions which convey resistance to a drug. You could culture cells in the drug...

8. Hot Plasmids - May 2022

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   ... live imaging application. Each visual barcode consists of a fluorescent protein coupled to a specific...

9. Building and Validating Recombinant Antibodies: A Case Study

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   ...validate your new construct by Sanger sequence analysis. But for this research, it was likely that the...

10. Antibodies 101: Buffers, Storage, and Conjugates

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    ...data in a manner that demands high accuracy and consistency?  If the answer to any of these questions is...

11. 15 Years of Addgene: The Top 15 Plasmids

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    ...customizable sgRNA from the U6 promoter and puromycin resistance from the EF-1a promoter. It’s a 3rd generation...

12. Multiplex Genome Editing with CRISPR-Cpf1

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    ...For cloning, Zetsche et al used four oligos that consist of direct repeats and crRNA.  Similar to a jigsaw...

13. Viral Vectors 101: Biosensors

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    ... cellular process. Cellular phenomena such as exocytosis, autophagy, voltage, and tension all have specific...

14. GCE4All: Making Genetic Code Expansion Accessible

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    ...essentially those needed to make site-directed mutagenesis, many researchers new to GCE aren’t able to successfully...

15. Viral Vectors 101: The AAV Retrograde Serotype

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    ... for understanding how the brain functions. The basis for these connections in the brain are (drum roll...

16. A Novice's Perspective on Neuroscience - SfN2017

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    ...seem to require some mathematical know-how for analysis. If you’re looking to get into neuroscience and...

17. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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    ...involved in cytoskeletal control, cell growth, and endocytosis. Recently, the Sidhu group at the University ...

18. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    ...transcription factor. It also gives cells antibiotic resistance to neomycin so that you can select the population...

19. How to Make Friends and Meet People at a Scientific Conference

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    ... Conference Dinner - by Julio Peironcely on the Thesis Whisperer "Not Networking - Building Relationships...

20. 3 Challenges in Plant Synthetic Biology

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    ...plant synthetic biology In addition to public resistance, an overall limited understanding of plant biology...