We narrowed to 731 results for: Sis;

1. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   ...polypurine tract; recognition site for proviral DNA synthesis. Increases transduction efficiency and transgene...

2. Viral Vectors 101: Biosensors

   Type

   Blog Post

   ... cellular process. Cellular phenomena such as exocytosis, autophagy, voltage, and tension all have specific...

3. GCE4All: Making Genetic Code Expansion Accessible

   Type

   Blog Post

   ...essentially those needed to make site-directed mutagenesis, many researchers new to GCE aren’t able to successfully...

4. From Lab Bench to Leadership: Transitioning into Management as a PhD

   Type

   Blog Post

   ...burdensome task of explaining the steps to a lab assistant. However, I recognized the need to shift my mindset...

5. Viral Vectors 101: The AAV Retrograde Serotype

   Type

   Blog Post

   ... for understanding how the brain functions. The basis for these connections in the brain are (drum roll...

6. How Addgene Creates Content

   Type

   Blog Post

   ...reviews on blog posts are done on a case-by-case basis, as many of our blog contributors primarily write...

7. Plasmids 101: Screens vs. Selections

   Type

   Blog Post

   ...trying to identify genetic conditions which convey resistance to a drug. You could culture cells in the drug...

8. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

   Type

   Blog Post

   ...transcription factor. It also gives cells antibiotic resistance to neomycin so that you can select the population...

9. How to Make Friends and Meet People at a Scientific Conference

   Type

   Blog Post

   ... Conference Dinner - by Julio Peironcely on the Thesis Whisperer "Not Networking - Building Relationships...

10. 3 Challenges in Plant Synthetic Biology

    Type

    Blog Post

    ...plant synthetic biology In addition to public resistance, an overall limited understanding of plant biology...

11. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type

    Blog Post

    ...company won’t go anywhere. The team should initially consist of 2-3 individuals that are aligned in vision and...

12. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type

    Blog Post

    ...programs. The groups in these programs typically consist of 1-2 mentors and 3-5 mentees who meet together...

13. CRISPR Library Amplification

    Type

    Protocol

    ... on incremental, ordered cell lysis. E. coli cells are subject to lysis by freeze-thaw if not suspended...sufficient DNA for use, pool samples, and continue with analysis. “Sufficient” DNA will vary upon intended use....

14. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type

    Blog Post

    ...the level of gene expression. For example, the synthesis of deoxychromoviridans requires three genes whose...

15. dTAG - You're it!

    Type

    Blog Post

    ...protein-of-interest, increasing target selectivity, overcoming resistance to inhibitors, and inducing prolonged biological...

16. RANbodies: Reporter Nanobody Fusions

    Type

    Blog Post

    ...secondary antibody. Chicken IgY This RANbody reporter consists of two chicken IgY domains. Chicken IgY domains...

17. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type

    Blog Post

    ...encoded, light-gated ion translocators to control tumorigenesis." Oncotarget 7.15 (2016): 19575. PubMed PMID...

18. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    ...Goedhart and Marieke Mastop! Joachim Goedhart is an assistant professor at the Section of Molecular Cytology...

19. Choosing the B(right)est Fluorescent Protein: Photostability

    Type

    Blog Post

    ...Goedhart and Marieke Mastop! Joachim Goedhart is an assistant professor at the Section of Molecular Cytology...

20. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type

    Blog Post

    ...degradation. Additionally, electron microscopy analysis is time consuming. GFP-tagged Atg8p or LC3: ...