...them in 1885. E. coli are mainly found in the intestinal tract of animals. There are many different naturally...coli used today are descended from two individual isolates, the K-12 strain and the B strain. K-12 was isolated...typically standard; just having the gene listed indicates it is non-functional. If a gene is deleted, it...

...expected fragments for that plasmid. This phenomenon indicates the presence of a multimer.   Fig. 1:... be used to detect multimers, since restriction sites in multimers usually repeat in tandem along with... of the plasmid monomer (see this article from BiteSize Bio for more information on how different plasmid...

...for the PI and lab members.  Make coffee or beer dates to talk to as many lab alumni as you can or schedule...or at least observe grant writing? Get equipment quotes? Understand administrative responsibilities? Is...this blog as a guide. Start a document to record notes and observations so you have data to review when...

... bioassay plates and remove bacteria. Use one scraper for all plates. Use two 10 mL pipettes (one for ...Antibiotic 245 mm bioassay plates (Molecular Devices, X602) Pro-Tip Pour these plates at least one day in advance...Agar + Antibiotic plates. Prewarm 12 mL recovery media at 37 °C (for at least 15 minutes). Prewarm 3X LB... Agar + Antibiotic plates at 37 °C. Prewarm 8X LB Agar + Antibiotic Bioassay plates. Prechill Micropulser...Micropulser cuvettes on ice. Thaw 4 tubes of electrocompetent cells on ice for 15-20 minutes or until completely...transformed cells on each of the eight bioassay plates (two plates per tube). Distribute evenly with a sterile... edge that can scrape plates more abrasively at a certain angle. Incubate plates upside down at 30 ℃ overnight...

...Each path to science policy is unique and this creates a rich and diverse workforce. Out of all the career...interests.  Action Items:  Sign up to receive updates from ESAL and explore their Local Engagement Playbook...others (see more in Figure 2). Starting early demonstrates not only your interest to future positions, ...

...aware of any possible alternative translation start sites or alternative splicing that could lead to some ...with your protein of interest and show just the faintest band from the cells transfected with the other...work great in another context! So the next time you test an antibody (perhaps one from Addgene!), consider...

...existing sites in the original vector. Designing oligos To add NdeI, PacI, AscI and MfeI sites between ...EcoRI and SalI sites of the vector, we design a top oligo with each of the additional sites in tandem ( ... to start at 95°C for 2 minutes. Then, gradually cool to 25°C over 45 minutes. Ligation Dilute 5μL of ...tutorial we will discuss how to add new restriction sites to the MCS of an empty vector. However, the following...set of oligos containing your desired restriction sites and add them to your existing vector. It is a couple...oligo), but this would destroy the EcoRI and SalI sites in the final vector. Order the following oligos ...Place tube in 90-95°C hot block and leave for 3-5 minutes. Remove the hot block from the heat source (turn...

...improvement over Cas9-mediated HDR for the loci tested, where average HDR-mediated editing frequency was...sequences, expanding the number of available target sites for base editing (Kim et al., 2017). For each base...have an improved base editing capacity, even at sites previously difficult to target. ABE8s can achieve...

...holiday spirit by kicking off our annual #DeckTheLab contest. The bar is set very high from last year’s impressive...some cheer by snapping a photo of you and your labmates celebrating the holidays and tweeting it with ...

... first edition of our Antibodies 101 eBook! The latest addition to our eBook collection is a handy resource...start looking for answers.    We hope you find the latest addition to our eBook collection useful. And, as...

...celebrate the 12 Days of CRISPR with some of the latest and greatest in CRISPR tools and resources! Ready to sing...

...Update Apr 8, 2021: While this contest is over, we will host other contests in the future. Subscribe to ...

... spent the last week perusing the Deck The Lab contest submissions on Twitter and Instagram and we’re ...these creative entries as the winners of the 2019 contest. Agar art For the second year in a row, agar ...

.... There is value, then, in having a guide that curates this information. And so I created the Codon Guide... genetic engineering curriculum at MIT. He also writes Codon, a blog about biotechnology.    ...

...be worn at all times. Use plastic pipettes in place of glass pipettes or needles. Liquid waste should be...shRNA oligos are cloned into the AgeI and EcoRI sites in place of the stuffer. The AgeI site is destroyed... 10x NEB buffer 2 35 μL ddH 2 O Incubate for 4 minutes at 95°C in a PCR machine or in a beaker of boiling...PCR machine, incubate the sample at 70°C for 10 minutes then slowly cool to room temperature over the period...following manufacturer’s protocol. Plate on LB agar plates containing 100 μg/mL ampicillin or carbenicillin...swirling or gently flicking the tube. Incubate for 5 minutes at room temperature. e. Add 80 μL of FuGENE® master...gently flicking the tube. f. Incubate for 20-30 minutes at room temperature. g. Retrieve HEK-293T cells...

...we can offer the scientific community the latest and greatest plasmid tools! Thank you depositors! In...

... some headphones to learn more about the latest and greatest plasmids through this podcast segment. Below...

...Plasmids offered as cloning grade DNA As we begin testing out this service, we’re starting with 210 plasmids...next generation sequencing and spectrophotometry to test purity (260/280 nm and 260/230 nm ratios) and measure...

...repository  Name Serotype Category Depositor  Notes pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources...

...repository  Name Serotype Category Depositor  Notes pAAV-CAG-emiRFP670 AAV1 Control Kiryl Piatkevich...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources...