We narrowed to 939 results for: TIM
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TypeBlog Post...monitor enzymatic activity or phosphorylation over time. You can also use biosensors to follow processes...Use to track a process (e.g. enzyme activity) over time or under various conditions Localization within...
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Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog Post...making a custom feature in Snapgene. This saves us time as we are often analyzing deposits with many plasmids... to quickly align multiple sequences at the same time. This means we can easily compare and analyze any... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog Post...base editor pCMV-BE3 has been requested nearly 400 times, and newcomer pCMV-ABE7.10 has already earned a ...for Bacterial Genome Engineering and CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR... -
FastCloning: A Newer, Simpler Cloning Technique
TypeBlog Post...vector purification, and, most importantly, excessive time requirements. FastCloning is a simpler yet reliable...place for an hour at 37°C. This should be enough time to completely digest the template DNA. Note—templates... -
Management for Scientists: Giving Feedback
TypeBlog Post...to become more effective and successful. Giving timely and useful feedback is an absolutely required aspect...this format and it becomes even more effective over time if you take it seriously. Person who will receive... -
Plasmids 101: Origin of Replication
TypeBlog Post...you have only one plasmid type in your cells at a time? Do you want to make a lot of your plasmid? Is the...streaked bacteria have higher copy numbers – for optimal results always pick a single colony and do not ... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post...tracing of the performance of each gRNA multiple times within the same experiment by counting both the ...restriction enzyme digestion of single molecules in real-time. Find plasmids expressing tTALE-FP Shin et al., ... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog Post... or yellow fluorescent protein at the time. It is 1.5 to 3 times brighter than the most commonly used ... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog Post... be spending less time and resources resupplying our current inventory and more time providing new materials... -
What the HEK?
TypeBlog Post...community, many cellular research tools have been optimized specifically for and in HEKs. These systems built...are immortalized, but cell health can degrade over time in culture. This can affect factors that could skew... -
Summer SciComm: Science Storytelling
TypeBlog Post... experiment has not worked…again…for the seventh time…. I find that once I sit down and talk to a scientist...the midst of all these storytelling devices, we sometimes forget the most important thing about science:... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog Post...neuroscience research and other disciplines, over time. The resource may also expand to include other affinity...collection to become more useful to everyone over time. Through our incredible community, including your... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog Post... replicate, and the transgene will be lost. Over time, bacteria containing the recombined vector backbone...post. Check yourself before you recA yourself. Optimize Growth Conditions To reduce recombination, it ... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog Post...genome editing toolkit. However, a challenge in optimizing base editing technologies across several cell... APOBEC- and Cas9-mediated Editing (ACE) in real-time. The reporter constitutively expresses eGFP to indicate... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog Post...individuals interested in ordering from ABRC for the first time, we recommend reaching out to us at [email protected] Arabidopsis to your class for the first time – we want to hear from you. Reach out to us at ABRCedu... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog Post... without the need for cellular HDR. Having had time to digest my notes, since the conference, I think...cut-to-mutation distance affects editing efficiency. Optimizing Donor DNA for Enhanced CRISPR Genome Editing ... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog Post...gene synthesis, many researchers now use codon-optimized versions of common coding sequence features, so... in commercial plasmids were inconsistent and sometimes clearly wrong. In an effort to be rigorous, we... -
Plasmid Cloning by PCR
TypeBlog Post... making a copy of a piece of DNA and at the same time adding restriction sites to the ends of that piece...both function in the same buffer, as this will save time later In our example, we will use EcoRI and NotI... -
Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog Post... that could be transferred between cells. At the time, these extranuclear agents of heredity were thought...the widely adopted name for these elements. At the time, the use of episome seemed appropriate, especially... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog Post...described in 1971,” McSweeney said. “But, it is still sometimes wrongly diagnosed as a seizure disorder.” In 2012... working on several genetic diseases at the same time. The limitation of budget restrained us from costly...