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  2. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...a reporter signal. Reporter signals may be electrochemical or light-based, with luminescent and fluorescent... being especially popular. We’ll give you an introduction to fluorescent biosensors, but keep in mind ...

  4. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...cell types. MEFs were reprogrammed into iEPs by retroviral expression of transcription factors Foxa1 and.... Adding Mettl7a1 to the standard Foxa1-Hnf4α retroviral overexpression cocktail yielded a three-fold ...

  6. What the HEK?

    Type
    Blog Post
    ...Culture Adenoviral Vector Production and Troubleshooting Additional resources on Addgene.org AAV ...  Susanna Stroik...

  7. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...endogenous IgGs, you should include no primary antibody controls in your experiments. If you do find high levels...resources on the Addgene blog Antibodies 101: Introduction to Immunofluorescence Antibodies 101: Affinity...

  8. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ... A fluorescent probe that releases an internal control Another autophagy biosensor is the GFP-LC3-RFP-LC3ΔG...it remains in the cytosol acting as an internal control. Autophagic flux is estimated by the GFP/RFP ratio...

  11. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...build, sales and marketing gives you a great introduction to the financial world. It teaches you about...you can find some great information on ELISA troubleshooting, ELISA kits and cell death assays. You can ...

  13. Cultivating Community Science at BosLab

    Type
    Blog Post
    ... the lab, so in December 2015 we held our first intro to bacterial transformation course (read our blog...bacterial transformation guru and instructor for our intro to biohacking courses. Our members pursue a diverse...

  15. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...YOUR PLASMID has been developed to teach gamma-retrovirus or lentivirus-based ex-vivo gene therapy’ strategies...at FRESCI particularly interested in advanced in vitro models technology. Follow him on twitter @FRE_SCI...

  16. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...person or virtually) will make the relationship stronger.  If appropriate, invite them to speak for your...chocolate-covered strawberries for helping with an introduction that helped someone get a job interview. Even...

  17. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...complex genetic diseases, Alzhiemer’s disease (AD).  Troy Rohn and his lab are researching a way to treat ...proteins aid the limitation of these mutations, to control the CRISPR system activity.  Using an AAV vector...

  18. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...Nsp5. Our set also includes a GFP construct as a control. For easier portability, all viral genes are flanked...clone selection. Expression is driven by a fairly strong EF1alpha promoter. We have verified expression ...

  19. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ... one figure. If you aren’t there yet, write introductions to potential publications or prepare a review...isn’t for a while. Getting started early on introductions and literature reviews could make the months...

  20. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...necessarily have to be. Regions of homology can be introduced to fragments via PCR using primers that contain...patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. Many thanks to ...
Showing: 601 - 620 of 914 results