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We narrowed to 750 results for: Uty

Showing: 601 - 620 of 750 results

  3. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...promoters, are always active and thus referred to as constitutive promoters. Others are only active under specific...

  11. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...different incubation options available. Caution Use the Stop Solution in a well ventilated area and avoid ...Immediately before use, mix 5 mL TMB Solution and 5 mL Peroxide Solution from the TMB substrate kit. Cap the...determine the ideal concentration. Prepare serial dilutions of the purified antigen as follows: 2 ng/µL : ... wells of each row, using a different standard solution for each row of a 96-well ELISA microplate. Cover...the wash buffer from the wells. Prepare antibody dilution buffer (0.1% BSA in PBS) as follows: Dilute 50... to the desired concentrations in the antibody dilution buffer. Pro-Tip The ideal antibody concentration...multichannel pipette, add 100 µL of primary antibody solution to the appropriate wells. Cover the plate with...

  12. Kit Free RNA Extraction

    Type
    Protocol
    ...Option #1 - Solution D Protocol Before starting this protocol, make sure to prepare solution D (see reagent...or lyse tissues or cells in Solution D. For tissues: use 1 mL of Solution D per 100 mg of cells. For cultured...resuspension. This protocol provides two options: using Solution D (see reagent section for recipe) or using an...all-in-one acid guanidinium thiocyanate-phenol solution such as TRIzol®. For the precipitation step, two...reagents RNase-free (Use an RNase decontamination solution, such as RNaseZap® or RNase AWAY®, may be used... Vortexer -20°C freezer -80°C freezer Reagents Solution D (for Protocol Option #1 ): 4 M Guanidinium Thiocyanate...alcohol (49:1) 75% Ethanol RNase-free water or TE solution RNase free tubes: microcentrifuge tubes, 4 mL ...

  13. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...viral dilution to each well (each well gets one dilution, so a 6-well plate will hold 5 dilutions plus ...just a sample of possible dilutions. You may want to try higher/lower dilutions depending on your downstream...the results of your titration. Mix the dilutions well. Dilution Volume of Lentivirus (μL) Volume of DMEM...that already contain 0.5 mL of virus solutions at various dilution. Make sure to use the polybrene-containing...batch of DMEM complete + 10 µg/mL polybrene by diluting 20 µL of 10 mg/mL polybrene into 20 mL media. ...aliquot on ice prior to use. Prepare a range of dilutions of the lentivirus in DMEM complete + 10 µg/mL ...polybrene-containing media to make the cell solution in this step. To seed the cells: Prepare a batch of cells as ...

  20. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...applications, smaller affinity reagents means higher resolution. Many of the below are also small enough to be...
Showing: 601 - 620 of 750 results