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...resources on Addgene.org  Addgene's Plasmids 101 eBookAddgene's Molecular Biology ReferenceAddgene's Restriction... performing restriction digests on plasmids at Addgene, we sometimes observe something odd in our uncut... the short-read Illumina MiSeq sequencing that Addgene uses.) However, when some researchers received ...described in this article.   Long-read sequencing Addgene uses Illumina MiSeq NGS to perform whole plasmid...Restriction Digest Protocol More resources on the Addgene blog:  Plasmids 101: What is a plasmid? Plasmids ... in the comments below or contact us at help@addgene.org. Resources and References References Bacolla...

...Science Communication Resources at Addgene.org Check out Careers at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...small tasks (like... preparing plasmids to send to Addgene). Following my advice from above, you should probably... in the comments! Additional Resources on the Addgene Blog Management for Scientists eBook Get Advice...

...Addgene Executive director, Joanne Kamens, recently participated in a Reddit AMA (short for “Ask Me Anything...content on many of the requested topics to the Addgene blog The blog has many other excellent posts on...and happy to help. Additional Resources on the Addgene Blog: 10 Ways to Share Your Science 6 Tools & ...How We're Sharing Science through Video with the Addgene Youtube Channel Check Out Vidoes of Gender & Science...

... for the generation of human disease models." Addgene Outreach Scientist and fellow blogger Caroline ...CRISPR Plasmids? CRISPR-Cas9 Plasmids available at Addgene CRISPR-Cas9 References, Protocols, and more References...

...Paolo Colombi, a product development scientist at Addgene. Cloning can be quite an arduous process. The PCR...currently the product development scientist at Addgene. In the lab he develops new assays and processes...possible aspects.     Additional Resources on the Addgene Blog Browse our Plasmids 101 blog posts Read blog...about different plasmid elements Resources on Addgene.org Find protocols for plasmid cloning Read our ...

...pigment. Find plasmids for blue-white screening at Addgene! Some bacterial strains contain lacZ in their genome... troubleshooting Sanger sequencing results. At Addgene, we now use next-generation sequencing during our...the correct clone. Additional resources on the Addgene blog Get all the plasmid basics in our Plasmids...molecular biology protocols and tips Resources on Addgene.org Learn more about Sanger sequencing Read the ...

...desired genomic edit.  Find SRIRACCHA plasmids at Addgene GEMCherry2, an improved monomeric fluorescent protein...stem cells.  Find GEmCherry2 reporter plasmids at Addgene Overall SRIRACHA and GEmCherry2 reporter systems.../jbc.m117.777722 Additional resources on the Addgene blog Browse all of our CRISPR blog posts Find ...with our CRISPR 101 blog posts Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for...

...resources on addgene.org  View Addgene's collection of empty gRNA vectors View Addgene's CRISPR resources...lentiviral plasmids Additional resources on the Addgene Blog  How to design your CRISPR gRNA CRISPR 101...

...Several OCaMP constructs are now available through Addgene. Whether you're working with cell cultures, slice..." %}{% raw %}"Find OCaMP plasmids available at Addgene!"{% endraw %}{% end_module_attribute %}{% module_attribute...2025.07.28.667269. Additional Resources on the Addgene Blog Viral Vectors 101: Calcium Sensors New Neuroscience...https://www.addgene.org/browse/article/28248224/","href_with_scheme":"https://www.addgene.org/browse/article...Fluorescent Protein Additional Resources on addgene.org Fluorescent Proteins: Biosensor Plasmids In-...

...added phosphoserine. A community resource Like Addgene, GCE4All is a community resource which depends ...researchers. One way of doing that is by using Addgene’s Blue Flame award to identify highly requested ...the plasmids and bacterial strains (available at Addgene for GCE work. Plus, keep an eye on our blog – we...

...resources Addgene’s viral services: AAV Resources on the Addgene blog Viral Vectors 101: Viral Vector...v13102073 Resources on Addgene.org Adeno-associated Virus (AAV) Guide Addgene’s viral plasmids and resources...

...resources on the Addgene blog Find our synthetic biology blog posts Resources on Addgene.org Find plasmids... pC013-Twinstrep-SUMO-huLwCas13a plasmids from Addgene, which would potentially allow a stable Cas expression...” by Benoit Giquel, an outreach scientist from Addgene. The talk highlighted the importance of reagent...

... wild type and mutant R bodies can be found at Addgene here. What can R bodies do for you? R bodies are...26814170. Find Plasmids from This Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., ...Central PMCID: PMC26518. Additional Resources on the Addgene Blog Read About Other Hot Plasmid Technologies...Plasmid Tools for the Repository Resources on Addgene.org Read our Hot Plasmids Articles Find Plasmids...

...science communication Resources on Addgene.org Learn how Addgene engages with the community ...BioTechniques.     Additional resources on the Addgene blog Find all of our science communication posts...

...  Additional resources on the Addgene blog Get started with Addgene's science career resources Browse...

...will make those new plasmids available through Addgene, and they hope others will do the same. “This isn...10.1186/1754-1611-8-7. PubMed.  Related Resources Addgene's Synthetic Biology Overview More Synbio Blog Posts...

...The complete GreenGate toolkit, now available at Addgene, also gives the option to stack several expression...motivations for making GreenGate available through Addgene,” Lohmann said. “We strongly believe the free sharing...

...line), armed only with viruses obtained through Addgene’s viral service and the methods sections of scientific...of performing and analyzing a gene edit using Addgene’s lentiviral CRISPR tools. Schematic overview...repeatability and bad news for a screen. Because Addgene viruses are provided at a specific titer, you can...Protocols 2007; 2(11): 2734-46. PubMed PMID: 18007609. Addgene Materials Used 1. lentiCas9-Blast (Plasmid #52962...PMCID: PMC4744125. Additional Resources on the Addgene Blog Genome-wide Screening Using CRISPR/Cas9 Deliver...Mouse Models Using CRISPR/Cas9 Resources at Addgene.org Generating Stable Cell Lines with Lentivirus...

... Resources on Addgene.org Learn how Addgene engages with the community on our Addgene gives back page...biology and society.     Additional resources on the Addgene blog Check out our series on careers in science...

...are many more useful viral vectors available at Addgene: Follow our viral vector blog posts for updates... Get the ready-to-use lentiviral prep from the Addgene viral service. CRISPR technology lentiCRISPR v2...PMCID: PMC4830361. Additional Resources on the Addgene blog Viral vector topic page Beginners guide to...guide to all things lentivirus Resources on Addgene.org Gamma-retrovirus Guide Lentivirus Guide ...