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We narrowed to 820 results for: cell;

Showing: 601 - 620 of 820 results

  1. CRISPR References and Information

    Type
    Collection
    ...interest and correspond to edited and non-edited cell populations. For more information, see the Hsiau...138 KB Musunuru CRISPRs in human pluripotent stem cells pCas9_GFP ; gRNA empty vector Protocol at StemBook...Bauer Protocol for Genomic Deletions in Mammalian Cell Lines pSpCas9(BB) (pX330) PDF, 330 KB Parrott NEBuilder...

  2. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ... that converts sucrose into a toxic metabolite. Cells that have undergone a double crossover will not ...contain sacB, allowing them to grow on sucrose. Cells that have only gone through the single cross over... from Harvard. Jake received his MD and a PhD in Cell and Molecular Biology from the University of Pennsylvania...Giacalone et al., 2006). The Chen lab demonstrated excellent counter-selection which enabled very efficient...desired deletion directly into cut vector with excellent efficiency using a commercial isothermal assembly...

  3. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...translation of many stop codons and detrimental effects on cell growth. The C321.ΔA strain was genomically recoded...is particularly interested in the intersection of cell signaling, metabolism, and the mighty mitochondrion..."The origins of protein phosphorylation." Nature cell biology4.5 (2002): E127-E130. PubMed PMID: 11988757...commonly cited that approximately one-third of cellular proteins are modified through phosphorylation ...

  4. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...EvolvR could also be a tool for lineage tracing cells that do not tolerate double-strand DNA breaks by...researchers to trace the progeny of a single starting cell. Additionally, multiplexing of guides allows EvolvR...

  5. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...Voigt CA, Ellington AD. Cell. 2009 Jun 26;137(7):1272-81. doi: 10.1016/j.cell.2009.04.048. PubMed. Find...with sophisticated gene expression controls. Most cell types do not respond to low-intensity light, so ...

  6. Typing CRISPR Systems

    Type
    Blog Post
    ...III CRISPR-Cas Immunity. Cell, 161(5), 1164–1174. https://doi.org/10.1016/j.cell.2015.04.027 Stella, G.,...and is able to target and edit RNA in mammalian cells. Type IV Type IV is the middle child (like me!) ...-Cas3 for genome-editing applications. Molecular Cell, 84(3), 463-475.e5. https://doi.org/10.1016/j.molcel...biotechnology toolbox for and beyond genome editing. Cell & Bioscience, 8(1). https://doi.org/10.1186/s13578...

  8. KLF Research Plasmids

    Type
    Collection
    ...regulators with diverse roles in processes such as cell proliferation, differentiation, inflammation, metabolism...

  9. Genetic Code Expansion

    Type
    Collection
    ...expanding the genetic code in bacteria and mammalian cells....codons, and tRNA synthetases are introduced into the cell on plasmids and the new amino acid is introduced...Once these plasmids have been introduced in the cells, the non-canonical amino acid can be incorporated...match the growth medium, ncAA concentration, and the cell lines used previously. Remember that the orthogonal... – GFP for E. coli or mCherry-GFP for mammalian cells. You should also express the protein with and without...tRNA synthetase for use in E.coli and Mammalian Cells. Many of the plasmids also contain one or more copies...

  10. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...quantitatively evaluating antibody staining, or counting cells are now routine, open-source software projects that...portable device for studying plankton using flow cells, has become an established alternative to high-priced...

  11. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...force to control protein expression in mammalian cells: easy to use biological tools are abundant! In this...tag prior to introduction of the construct into cells. However, if you would like tunable control of an...

  13. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ... time and are potentially heritable in dividing cells. CRISPR Activate and Interfere technologies also...

  16. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...strain, the promoter being used should be tested in cell lines to determine how it impacts the expression...interest. Cre may be expressed globally (in every cell of the mouse) or in a tissue-specific manner (with...

  17. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...allow scientists to express a gene of interest in a cell or animal model. AAV plasmids contain several important...Integrity on rAAV8 Production Using the Baculovirus/Sf9 Cells System. Hum Gene Ther Methods, 28(5), 277–289. https...

  19. Fluorescent Proteins: FRET

    Type
    Collection
    ...influenced by many factors, such as the organism or cell type, relative position and orientation of the fluorophores... (Unpublished) FRET Indicators - Live and Fixed Cells Additional Resources at Addgene Learn more on the...

  20. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    ...annealing to a complementary ssDNA target in the cell.  Only Beta expression is required for recombineering...definitely the easier of the two options, but these cells are prone to mutations and will have more unintended.../Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR Methods for Bacterial Genome Engineering ...
Showing: 601 - 620 of 820 results