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Showing: 601 - 620 of 960 results

  1. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...deliver the appropriate isoform(s) of a given gene, as, many times, the isoform(s) common to a particular...Tuttle is a medical student at Tufts University and former researcher at the Wyss Institute for Biologically...

  2. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...model in medical research with an archive of information and data that has yet to be produced with mice...Arabidopsis. There are also important tips for plant transformations and the use of Arabidopsis in the classroom...functional genomics study in bacteria with superior performance. Nature Communications, 9(1). https://doi.org...

  3. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...rely on exogenous enzymes, require multiple transformation and plasmid curing steps, and can generate ... markers for allelic exchange. To do this, we performed the following modifications to pDS132 (Philippe...Hospital where he is now an attending physician. He performs post-doctoral research in the lab of Matthew Waldor...

  4. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ... GFP is such a well-validated, all-round good performing probe, it is the first choice when selecting ...Zacharias et al., 2002). GFP needs oxygen Chromophore formation requires a fully folded beta-barrel structure,...C-terminal fusions (Adjobo-Hermans, 2011). Structural information facilitates the choice of the proper site, although...

  5. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...generate non-phosphorylated recombinant protein and perform in vitro kinase reactions. Often, however, researchers... serves as an intermediate on the path to the formation of cysteinyl-tRNA (4). The discovery of a phosphoserine...PMC4383998. 3. Li, Tong, et al. "Cysteinyl‐tRNA formation: the last puzzle of aminoacyl‐tRNA synthesis."...

  6. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...are wild-type unless indicated otherwise fhuA (formerly tonA) ferric hydroxamate uptake, iron uptake receptor...Propagation of ccdB-containing plasmids Hte   High transformation efficiency deoR constitutive expression of...For a more comprehensive list and additional information, visit OpenWetWare's E.coli genotype resource...

  7. A Control for All Seasons

    Type
    Blog Post
    ...different types of controls and be able to use this information to design thoughtfully and thoroughly controlled...be due to natural biological variation. This information is critical for correctly interpreting experimental...experimental technique before starting. For more information about antibodies and antibody-based experiments...

  8. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...T7 promoter upstream of the left homology arm, perform in vitro transcription, and use the IVT product... and infundibulum. In vivo electroporation is performed immediately after injection. Image from Ohtsuka...contrast to standard microinjection, i-GONAD is performed directly on pregnant female mice using a small...

  9. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...thought that it was necessary to develop a single platform to connect talented professionals (who migrated...nominations for government fellowships. The closing and formal evaluation of the pilot program IMP 2014/2015, ... success of this program. As we build a base of former mentees, we encourage them to get involved and ...

  10. Finding Your Science Policy Path

    Type
    Blog Post
    ...societies, and industry through convening activities, formal lobbying, or strategy setting.  The “little p” ...failures, and challenges you’ve overcome. This information may help you determine what parts of your current...current job you want to keep or change and can inform your move into science policy. Read broadly about...

  11. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ... to consider how dyes can influence receptor conformation and signaling activity​​ (Sridharan et al., ... which is key to observing interactions and conformational changes in proteins. Both short distances of... expand the self-labeling capabilities of this platform. The Janelia Fluor ligands provide brighter and...

  12. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...Systemic capsids While there is quite a bit of information available on how to use systemic capsids, when...considerations for their use. Then, we’ll provide information about commonly used and emerging capsids to help...AAV1-X1 has not been tested in rats. For more information on reagents and protocols, visit https://clover.caltech.edu...pigtail macaque brain slices. Similarly, AAV9-X1.1 outperformed the other capsids in the rhesus macaque and ...

  13. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ... 2.3 fold decrease in indel formation. To further decrease indel formation 1.5-2 fold, the team fused ...displays highest editing efficiency but higher indel formation than BE2 Nishida et al., 2016 Target-AID   Edits...

  14. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    ... help you make sense of all the viral vector information that's out there, Addgenie Leila Haery has summed...Viral Vector Blog Posts from Addgene Need more information on viral vectors? Check out our viral vector ...

  15. Antibodies 101 Series on TikTok

    Type
    Blog Post
    ...antibodies? Our new antibodies animation has all the information! Link in bio. #STEM #antibodies #molecularbiology...whenever a new video is posted! If TikTok isn't your platform, all of our videos will also be shared on our ...

  16. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    ...Mani is a graduate student at Harvard University, former Co-director of Science in the News, and member ... received her PhD from Harvard University, is a former Co-director of Science in the News, and is a member...

  17. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    ...improving access to useful research materials and information,” we love to share lab protocols that we use ...unloading of the column and give some tips for performing this purification.  Check out the AAV Purification...

  18. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    ...under the Other dropdown menu. You can also find information on using Addgene’s website, the latest Addgene...Articles from guest bloggers will have the author information at the top and bottom of their article. We hope...

  20. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    ...detection Feng Zhang’s lab recently developed a platform called SHERLOCK (specific high-sensitivity enzymatic...plants, it has been difficult to compare the performance of different nucleases. Nicola Patron’s lab compared...
Showing: 601 - 620 of 960 results