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We narrowed to 971 results for: lars

Showing: 601 - 620 of 971 results

  3. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...search for "CRISPR", short-hand for Clustered Regularly Interspaced Short Palindromic Repeats, in Pubmed...Matt Ferenc, to organize the CRISPR forum's most popular questions and answers. Here are just 4 of the CRISPR-Cas9...introduce cleavage of the target site with efficiency similar to using a single chimeric gRNA. At the same time...

  4. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...low concentration or you are cloning an extremely large insert. Since the standard ligation reaction is...Central PMCID: PMC52876. 2. Novel approach to molecular cloning and polynucleotide synthesis using vaccinia...Plasmid Cloning Protocols Catch Up On Your Basic Molecular Biology Techniques Watch Our Protocol Videos ...

  5. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...problem, Gao et al. searched for Argonaute orthologs similar to TtAgo and PfAgo, another thermophilic Argonaute...variety of mammalian cell lines, with an efficiency similar to that of Cas9. Like Cas9, NgAgo is also able ...cleavage efficiency. If this finding is replicated in larger studies, it will represent a key advantage over...

  6. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...of Addgene's top blog posts from 2015 Our most popular posts continue to be those from our Plasmids 101...regardless of their end goals and believe the popularity of our career posts shows that we are doing just...Ramsden from UNC Chapel Hill explain some of the molecular gymnastics behind DNA repair by non-homologous...

  7. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...undeniably difficult connection to study on a molecular level. The brain is constantly influenced by signals...organisms, including the Anikeeva lab’s work on a similar neural fiber (Park et al., 2019). This provided...., Anikeeva, P., & Chandrakasan, A. P. (2020). Modular Optoelectronic System for Wireless, Programmable...

  8. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ...but for viral vector core facilities producing a large number of viral preparations in parallel, there ...Single-stranded Virus Next-generation Sequencing. Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org...Batch-to-Batch Consistency Evaluation of AAV Preparations. Molecular Therapy 25:1375–1386 . https://doi.org/10.1016...

  10. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...that cells with common ancestors tended to have similar reprogramming outcomes. For example, cells with...with shared CellTagD13 tags often had similar gene expression pattern changes and reprogramming outcomes...reprogramming is not stochastic, but rather there’s a particular cell state that’s permissive to reprogramming.In...

  12. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    ... Syndrome One of those teams is led by Claudio Aguilar (right) at Purdue University and is working to ...ago, but there is still no treatment available. Aguilar is a basic cell biologist. He got interested in...meet a few patients that suffered this disease,” Aguilar said. “Once you have that, there is no coming back...

  13. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...individuals in their 20’s who’ve built multibillion dollar tech companies using only laptops and some server...story altogether? The unfortunate answer is yes, largely due to a few limiting factors.  To start, the average...different than what was originally planned, due in large part to the feedback we received from our network...

  14. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ...eight hours worth of hands-on time. Creating a modular, selection-based gene tagging approach Multiple...promoter-FP fusion for the gene of interest. L1/L2 larvae from these strains are then heat shocked to remove... this system Dickinson et al. have developed a modular system that requires very little hands-on labor...

  15. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...Regulating translation is key to cellular function, especially during development or stress. With ribosome...explore the complexity of translational control in cellular physiology. Ribosome profiling Ribosome profiling...all of the mRNA bound-ribosomes in a cell. When cellular mRNAs are digested with ribonucleases, ribosomes...

  16. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...FLiPs in a variety of research applications and particularly notes their ability to be precisely spatio-temporally...hopes that other researchers will be able to use similar designs to create a wide variety of enzymes and...proposes using this sensor to detect HMOs found on cellular surfaces. After incubating the sensor with bacteria...

  17. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...affected the stem cell community – a community largely created due to Yamanaka’s seminal work. It didn't... prominent role in the scientific endeavor – particularly in fields like stem cell biology where questions...about his clinical trial patients with advanced macular degeneration; Laurenz Studer discussed his work...

  18. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...Esvelt’s CRISPR/Cas9 gene drive paper was very popular. People kept asking if we were teaching classes...provide all our members the opportunity to do molecular biology, we’ve developed community projects. Recently...protocols so that anyone can engineer yeast to do similar things. The focus is to make yeast engineering ...

  19. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ...being used to study SARS-CoV-2. The resurgence in popularity of the hACE2 transgenic mouse strain, for example...murine ACE2 (Subbarao et al., 2006). Now that a similar phenomena is appearing with regards to SARS-CoV...SARS-CoV-2, this mouse strain is regaining popularity. The Jackson Laboratory, a nonprofit research institution...

  20. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...problem can be attributed to communication, and in particular communication about experimental methods and ... and funding, so institutions and funders, to a large extent, hold the keys to getting transparency and...journals including Trends in Cell Biology, Cell, Molecular Cell, Developmental Cell, and Cell Stem Cell.  ...
Showing: 601 - 620 of 971 results