We narrowed to 779 results for: mos

1. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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   ...promoter is neuronal specific (Haery et al., 2019). Most AAV transfer plasmids have a promoter, but this ...

2. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

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   ...of our quality control (QC) mantra is to do our utmost to ensure that scientists are receiving the exact...

3. Single-cell tracking of lineage and identity with CellTag

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   ... reprogrammed and re-expressed fibroblast genes. Most cells ended up taking the ‘dead-end’ path. The CellTags...

4. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

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   ...tTA leads to a reduced expression level of XFPs, most likely by suppressing transcription. It is critical...

5. Addgene Begins Distribution of Recombinant Antibodies

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   ...to work with all of you to make this the best and most accessible recombinant antibody collection for advancing...

6. Antibodies 101: Epitope Availability

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   ... might suddenly work that didn't before. This is most often an issue with monoclonal antibodies, which...

7. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

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   ...everything that can go wrong with a plasmid. One of the most frequent problems that I help troubleshoot involves...

8. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

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   ...data with you. “Affinity reagents are among the most commonly used reagents in neuroscience research ...

9. Science Careers: Unruly Interests Feed Many Paths

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   Blog Post

   ...of comparative anatomy showed me that some of the most fascinating biology is found in weird and rare organisms...

10. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

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    Blog Post

    ...culprits are endogenous IgGs in your tissue sample. Most common IHC protocols use two antibodies - one against...

11. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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    ...both safety and usefulness of the tool. In fact, most gutted genomes have as much genome removed as possible...

12. Antibodies 101: Chimeric Antibodies

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    ...different species and/or isotype (Liddell 2013). In most cases, the resulting chimeric antibody retains the...

13. Viral Vectors 101: Transductions

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    ... a lipid-based or electrical-based transfection, most of the nucleic acid transfected will not stably ...

14. Using Addgene's New Viral Service for Your Research

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    ...Service Suggestion Form. Lentivirus As some of the most popular lentiviral vectors in the repository are...

15. New and Upcoming Viral Vectors - December 2019

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    ...packaged in AAVrg. jGCaMP7s, the variant with the most sensitive response to action potentials, is now ...

16. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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    ...exciting to test this approach with different capsids. Most importantly, because our AAV yields are improved...

17. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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    Blog Post

    ...from the bio-industry’s vision of ‘biofactories’, most successes in metabolic engineering have been limited...

18. Hot Plasmids: FRET-Based Biosensors

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    ...transfer (FRET)-based calcium biosensors. Perhaps most notably, the Twitch calcium sensors, which are available...

19. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

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    Blog Post

    ... basically every branch on the tree of life. Our most recent paper in Nature Methods offers the first ...

20. Creating accessible biology activities in schools with BioBits

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    Blog Post

    ...with FD-CF reactions affordable to many schools. Most importantly, experiments for FD-CF reactions can...