We narrowed to 928 results for: mpl

...necessary” detail after “necessary” detail and end up completely overwhelming your audience - like the professor...intuitive that actually wasn't. Maybe I needed to employ a visual aid instead of describing something verbally...group was just really tired and needed a slower, simpler presentation than I had planned; other times students...

...several different species are present. One common example is using pre-adsorbed secondary antibodies (which...antibodies are polyclonal, as their function is to amplify the signal from a primary antibody; the ability...target protein thereby greatly increasing signal amplification.  When to stay away from polyclonal antibodies...

...preserves RNA structure and naturally occurring complexes present in the cell, it can also introduce non-physiological...non-physiological RNA-protein interactions due to sample processing. Require prior knowledge that the protein...levels seen in HHCS patients. These are just three examples of how RaPID can be used to study RNA-protein ...

...intensive to process a large number of microbiome samples. To enable large-scale, longitudinal studies of...and can approximate mRNA concentration within a sample. Using this system, the Collins lab was able to...lab validated their platform on clinical stool samples by comparison to qPCR and demonstrated that it ...

...show. After graduating with a master’s, Susan completed a science writing internship with the National...planning.” To beef up her resume, she decided to complete a certificate in Strategic Communications and ...the most satisfying parts of the job is having completed one of these difficult pieces: “It’s probably ...

... the LoxP sites, which is more difficult than a simple knock out. Looking for some permanent knock out...two varieties: tet-on and tet-off. As the names imply, tet-off turns off constitutively expressed proteins... tunable control These systems can be easily implemented for exogenously introduced proteins, as many ...

...the take rate of mouse xenografts after cell implantation. Their goal was to figure out how to optimize...barcodes in the population (all barcodes can be amplified using the same sets of forward and reverse primers...dynamics in response to cancer therapy using high-complexity barcoding." Nature medicine 21.5 (2015): 440-...

...loxP, black arrowheads) and one mutated pair (for example- lox511, white arrowheads). It is necessary to ...express GeneR only in the presence of Cre. In this example, Cre would first invert the reporter and GeneR ...the presence of Cre. A similar strategy can be employed for "Cre-off" (i.e. your gene of interest starts...

... the xanthine produced via complete demethylation of caffeine. For example, the Barrick lab was able to...living biosensor for measuring caffeine content in samples. The Barrick lab and the UT Austin undergraduate...

...treatments, but are not useful for genetic engineering. Examples of outbred strains include Swiss Webster and Harlan... are injected into a blastocyst which is then implanted into a female animal for gestation. Nucleases ...are microinjected into a zygote, which is then implanted into a female mouse for gestation. All three can...

...animal models of disease and cultured cells. For example, in 2013, Jinsong Li and colleagues chose to explore...trials is somewhat similar (Hussein et al). It employs Cas9 to cut at two sites within the viral genome...current delivery technologies are suited for. For example, lipid nanoparticle delivery tends to be trafficked...

...In order to understand the complex wiring of the brain and the underpinnings of neurological disease,...while resulting in minimal off target effects. For example, if your receptors are expressed in the brain, ...receptor acts through. The DREADD hM3D(Gq), for example, lowers the threshold for neuronal signaling and...

...predecessors. Changes were implemented and I had to adapt accordingly. For example, the quarterly and annual...volunteered for various tasks as things came up. For example, I updated some sections of the NPA’s website. ...

... in proximity by the looping of chromatin, the complex of DNA and proteins that forms chromosomes. These... of the CAPTURE method. After protein and DNA complexes are cross-linked, DNA is digested into smaller...and 2) gRNAs Number of loci targeted One Many Sample type(s) Cell lines Cell lines, primary cells ...

... this purpose.Learn about the effects of CO2 on sample pH Cell morphology: Shape and size matter You should...which multiplies every 16 hours). So if you, for example, split cells on a Monday diluting them 1:10, you...contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage numbers Keep an eye on the...

...targeting anywhere in an exon. But things get more complicated if you’d like to use homology directed repair...alternative PAM sequences are longer and often more complex than NGG, resulting in lower overall genome frequency... be less predictable than SpCas9 off-targets. Employing xCas9 for transcriptional regulation and base ...

... an enhanced pU6cm promoter, viral replicon amplification of cargo, and heat treatment.  Figure...detection of activation triggered by endogenous GPCR. Example applications include large-scale pharmacological...recombinant antibody in human postmortem brain samples. Results show positive staining in tissues from...

...specific type of virus you are trying to make. For example, bacteriophages will need to be produced in bacteria... P0), infecting new Sf9 cells, and getting an amplified P2 virus. This P2 virus can be produced in a greater...Processing of your baculovirus is straightforward — simply spin down the supernatant in a centrifuge to remove...

...RNA (pegRNA). The pegRNA consists of a target-complementary spacer, a scaffold sequence, and an extension...primer binding site and a reverse transcription template (RTT). The design of the pegRNA has been shown... of different pegRNAs for each desired edit, it simply becomes impossible (or at least unreasonable) to...

...specific epitope that your antibody binds. For example, Myc1-9E10 binds EQKLISEEDL (pronounced “equi-kli-seed-le...be the antibody Mary Poppins!   All of the examples of new versions of Myc1-9E10 mentioned above basically...for the foreseeable future. These newer findings simply add to our understanding of how this antibody works...