Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 938 results for: mpl

Showing: 601 - 620 of 938 results

  2. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ...help my research and my career. Here are three examples of how using social media has helped me or my ... can start using Twitter and provide real-life examples of how they and others have used Twitter professionally... to use LinkedIn to research potential future employers and to arrange informational interviews. 4. “Tips...

  3. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...reliable and reproducible. However, we do not use amplicons in ITR as we have found this to strongly bias ...somewhat more reliable quantitation. Differences in sample treatment across methods can affect reported titer...that users keep titering method consistent across samples within a project. Targeting Your AAV Q4: Are there...

  4. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...specific region of your protein of interest. For example, if you are studying a protein that may bind a ...recognize the protein even though it is present in your sample. Additionally, there is a lower likelihood that...higher than that of polyclonal antibodies. For example, it takes longer to develop a hybridoma than it...

  5. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...chromosome your transgene has integrated. This can be accomplished with a few matings to balancer chromosome lines...throughout the lifespan of the fly to determine the implications of your genetic manipulations. You can produce... (2). Metabolic assays such as Seahorse can be employed to look at oxygen consumption. Drosophila are ...

  6. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...that are related to the imaging strategy and the sample. Practical brightness A protein with a high intrinsic... future application. In figure 2 we provide an example of the comparison of the practical brightness of...to this data. The practical brightness assay (employing co-expressing fluorescent proteins or using endogenous...

  7. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...2004. We’ve already distributed over 500 viral samples to scientists all over the world. Now that the ...donations. That seed money was used to build a completely self-supporting service so that Addgene is not..., tracked production and distribution of viral samples. The Office Team made sure we could ship internationally...

  8. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...transfection step of the viral packaging cell line. For example, Phoenix, a second generation γ-retrovirus packaging... to enter the nucleus through the nuclear pore complex. This feature may be useful for the transduction... the components found within the backbone. For example: pLXSN has the wild type LTR, an MCS for cloning...

  9. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...cool because they can transmit information, for example, from one of the 180 regions to another or from...the brain to the rest of the body. Therefore, by simply looking at how neurons are physically connected...even further defined population of neurons. For example, say I wanted to access all the neurons that signaled...

  10. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...applications, while electroporation of a human, for example, isn’t a reasonable option. Since viruses used...infect both non-dividing and dividing cells. They accomplish this through nuclear localization signals which... immunodeficiency virus type 1 preintegration complexes.” Proc. Natl. Acad. Sci. U.S.A. 89 (1992) 6580...

  11. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...binding of a new inactive heterotrimeric G protein complex and subsequently another round of signaling.    ...finally, to a response? Let’s take a look at a few examples with plasmids deposited at Addgene.  PRESTO-TANGO...gamma subunits of the heterotrimeric G protein complex that can be used to detect G protein activation...

  12. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...students focus on the application process, and simplify access to academic resources, we created STEAMid...worried about any eligibility provision, don’t guess. Simply reach out to the program coordinator with your ...Chemical Biology Program at Harvard. Roo wants to simplify access to academic resources among economically...

  13. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...intensive to process a large number of microbiome samples. To enable large-scale, longitudinal studies of...and can approximate mRNA concentration within a sample. Using this system, the Collins lab was able to...lab validated their platform on clinical stool samples by comparison to qPCR and demonstrated that it ...

  14. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...necessary” detail after “necessary” detail and end up completely overwhelming your audience - like the professor...intuitive that actually wasn't. Maybe I needed to employ a visual aid instead of describing something verbally...group was just really tired and needed a slower, simpler presentation than I had planned; other times students...

  15. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...several different species are present. One common example is using pre-adsorbed secondary antibodies (which...antibodies are polyclonal, as their function is to amplify the signal from a primary antibody; the ability...target protein thereby greatly increasing signal amplification.  When to stay away from polyclonal antibodies...

  17. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ... practice to compare degradation rates between samples treated with or without an autophagy inhibitor....accumulates in the vacuole. See Figure 2 for an example of what fluorescence looks like for cytoplasmic...Fluorescent Protein Oligomerization Seeing Red: Simple GFP Photoconversion Light Sheet Fluorescence Microscopy...

  18. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ... the LoxP sites, which is more difficult than a simple knock out. Looking for some permanent knock out...two varieties: tet-on and tet-off. As the names imply, tet-off turns off constitutively expressed proteins... tunable control These systems can be easily implemented for exogenously introduced proteins, as many ...

  19. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...preserves RNA structure and naturally occurring complexes present in the cell, it can also introduce non-physiological...non-physiological RNA-protein interactions due to sample processing. Require prior knowledge that the protein...levels seen in HHCS patients. These are just three examples of how RaPID can be used to study RNA-protein ...

  20. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...the take rate of mouse xenografts after cell implantation. Their goal was to figure out how to optimize...barcodes in the population (all barcodes can be amplified using the same sets of forward and reverse primers...dynamics in response to cancer therapy using high-complexity barcoding." Nature medicine 21.5 (2015): 440-...
Showing: 601 - 620 of 938 results