Addgene

We narrowed to 758 results for: ncl

Showing: 601 - 620 of 758 results

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CRISPR 101: Non-Homologous End Joining

Type

Blog Post

...NHEJ has a vast toolbox of processing factors, including polymerases (Pol μ and Pol λ), nucleases (Artemis...
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

Type

Blog Post

...Further Reading: For more information about SAM, including a tool for getting optimized guide sequences for...
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

Type

Blog Post

...Mike recommends that the team should initially include a visionary, a science-minded individual, and a...
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

Type

Blog Post

...for one or both of these criteria. If you can, include people from different labs, departments or organizations...
RaPID Detection of RNA-protein Interactions

Type

Blog Post

... for RaPID experiments RaPID negative controls include both scrambled RNA motifs and cells transfected...
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

Type

Blog Post

...to nine proteins in ten ddifferent cell types, including difficult-to-manipulate primary cells, achieving...
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

Type

Blog Post

...skills that Susan finds important for her work include: “Listening. Evaluating evidence. Critical thinking...
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

Type

Blog Post

...or the molecular weight shift of Pho8 from its uncleaved to cleaved form is the final read out of this ...
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

Type

Blog Post

...cancelling plans are going ahead with raises anyway, including the entire faculty of the biochemistry department...
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

Type

Blog Post

... currently available for approaching it. These include the traditional restriction enzyme/ligase-mediated...
Troubleshooting Your Plasmid Cloning Experiment

Type

Blog Post

... was acknowledged in hundreds of publications including Cell, Nature, and other journals. Paolo Colombi...
The Breast Cancer Microenvironment: A Tumor’s Backstage Team

Type

Blog Post

...myoepithelial cells prevented progression to malignancy, concluding that these cells may be considered "natural tumor...
What's New in CRISPR - November 2021

Type

Blog Post

...and human cells using various delivery methods, including plasmid, ribonucleoprotein, and AAV. Read the...
Antibodies 101: Conjugation

Type

Blog Post

... 4) Modifications made to antibodies, such as inclusion of non-canonical amino acids or peptide tags. ...
Plasmids 101: The protein expression toolbox

Type

Blog Post

...once the ligand is removed. Degron tag systems include small molecule assisted shut off (SMASh), auxin-inducible...
Giving gRNAs a Facelift - Synthetic and Beyond

Type

Blog Post

...Other useful backbone modifications to consider include amide linkages, unlocked nucleic acids, and a constrained...
Transferable Skills Guide: Cross-team Communication

Type

Blog Post

...Annotate: When creating important documentation, including SOPs or project-related documents, we define new...
Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

Type

Blog Post

...decrease these so-called “off-target effects” have included using a Cas9 nickase variant, lowering Cas9 expression...
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

Type

Blog Post

... Someone could take on an engineering project including hundreds of genes and regulation and resource ...
Plasmids 101: Cre-lox

Type

Blog Post

...This has been used for a wide range of purposes, including activating an oncogene only in a particular organ...

Showing: 601 - 620 of 758 results

Results per page:

CRISPR 101: Non-Homologous End Joining

Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

RaPID Detection of RNA-protein Interactions

Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

Troubleshooting Your Plasmid Cloning Experiment

The Breast Cancer Microenvironment: A Tumor’s Backstage Team

What's New in CRISPR - November 2021

Antibodies 101: Conjugation

Plasmids 101: The protein expression toolbox

Giving gRNAs a Facelift - Synthetic and Beyond

Transferable Skills Guide: Cross-team Communication

Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

Plasmids 101: Cre-lox

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