Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 932 results for: omp

Showing: 601 - 620 of 932 results

  1. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ... light sensitive and have enhanced conductance compared to current channelrhodopsins. Their new channelrhodopsins...neurons do not express as many copies of the ChRs as compared to direct injection. This lower multiplicity of...better suited for systemic delivery with PHP.eB compared to the existing ChR2(H134R).  In the second experiment...

  2. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...Stanford University. Sex and gender are critical components of biological research that are often forgotten...cost at this early stage of research is minimal compared to that of human clinical trials. And, as Janine...studies that draw from a body of research that is incomplete (because sex was not analyzed) may harm humans...

  3. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...Cloner, and Snapgene), and save these to a shared computer or server. This will ensure that future members...more than two sequencing reactions. In the more complex cases, we sometimes have to design new primers ...saves us the step of purifying the DNA and gives comparable success rates and sequence quality, which is ...

  4. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...response against AdV. Importantly, recombination-competent adenovirus (RCA) can be generated if the E1 gene...negligible toxicity. However, their production is more complex and the presence of helper virus contaminants have...amplification.*Pro-Tip*: The cells will become completely confluent and the media will turn yellow. Do ...

  6. Lab Automation at Addgene

    Type
    Blog Post
    ...allowing us to do up to 96 transformations in one run compared to doing about 50 transformations in the same ...increased plasmid demand it was too time consuming to complete this process by hand and we welcomed yet another...liquid handler can fill 400 vials in 35 minutes compared to a lab assisstant filling about 200 vials in...

  7. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...machinery.   Well, I’ve got some good news: complete DNA demethylation can be achieved by dCas9 alone...too.  High dCas9 and gRNA expression are key for complete DNA demethylation – tight binding is crucial for...Charlton J, Jung EJ, Mattei AL et al. (2020) TETs compete with DNMT3 activity in pluripotent cells at thousands...

  9. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...difficult to functionally manipulate and study in comparison to protein-coding genes (Rinn and Chang 2012)...deletions and insertions in the DNA sequence can completely disrupt expression of the transcribed ncRNA (...interactions between ncRNAs and adjacent genes are complex and not yet fully understood, so it is important...

  10. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...itself. Among other things, science communication comprises broadcasting, science writing, and certain aspects... get on stage for a Science Slam – a sort of competitive TED talk – to win the audience’s applause with...photography and participate in a science photo competition. Or nominate yourself as an educator for a short...

  11. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...get the molecular components of CRISPR/Cas9 (Cas9 protein and the crRNA-tracrRNA complex) and the donor ...destabilizes the cell’s outer membrane and allows the components to physically enter the cells so they can go ...

  12. CRISPR in the Clinic

    Type
    Blog Post
    ...well as the introduction of the cancer-fighting component, CAR. Clinical trials are underway to explore ... have involved turning on fetal hemoglobin to compensate for defects in the adult hemoglobin produced ...for use in the next few months. Several other companies have followed suite, with Beam Therapeutics initiating...

  13. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...choosing antibodies   Complexity is a key factor Biological systems tend to be complex with a variety of ...proteins interacting. Experiments tend to be equally complex with users trying to probe multiple targets in ...

  14. Engaging with science and society at pgEd

    Type
    Blog Post
    ...of science, even while I was a bench scientist completing my graduate studies in molecular biology and ...for the past three years. pgEd is a small team comprised of individuals from diverse backgrounds and disciplines...social context. This “general public” audience comprises the taxpayers who fund much of scientific research...

  15. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...structure of the hairpin-DNA complex is thought to destabilize the transcription complex, initiating cleavage ...mechanism, whereas Polymerase II termination is more complex and involves two RNA polymerase-associated proteins...

  16. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    ...who are usually antagonistic and impolite, Complainers, who spend most of their time moaning about their...with negative comments (hostile-aggressives, complainers, know-it all experts, wet-blanket negativists...support from your colleagues or other supervisors to complete your work. Whether your supervisor is a negative...

  17. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ... fluorescent signal throughout the reaction is compared to a standard curve of amplified DNA of a known...The cycle in which the unknown DNA is detected compared to the standard curve can be used to determine... to note however, that multiplex PCR is more complicated to develop and is often less sensitive than PCRs...

  18. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ... strategy uses new combinations of PE protein components (Figure 1), an altered epegRNA delivered with...protein-coupled receptors (GPCRs) are critical components of many physiological pathways and represent ...et al., 2024). Each plasmid expresses all the components of the biosensor in optimal proportions from ...

  19. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...screens have gotten larger, easier, and more comprehensive thanks to the consistent development and improvement...vectors is how the cargo — in this case, the CRISPR components — are expressed. Unlike lentivirus, cargo delivered... away from the comfort of Cas9, and opt for a completely different Cas effector, like Cas12a (Cpf1). Even...

  20. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...plants, mammals, and insects. In this post, we will compare two of the most widely used systems, mammalian ... from dedicated adenoviral production labs or companies. Production of both AAV and lentivirus requires...10430340260355347. Vaughn, J. L., Goodwin, R. H., Tompkins, G. J., & McCawley, P. (1977). The establishment...
Showing: 601 - 620 of 932 results