We narrowed to 761 results for: rest

1. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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   Blog Post

   ... also need to be transfected into the cells of interest. In contrast, RNA guides must be expressed from...

2. Addgene's Top 10 Blog Posts of 2015

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   Blog Post

   ...strains you can use to express your protein of interest. 4. Plasmids 101: Blue-White Screening Getting...

3. Trading Spaces - Visiting Scientist Edition

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   Blog Post

   ...If you’re interested in learning a new skill, getting access to equipment you don’t have, or maybe both...

4. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

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   Blog Post

   ... is opened and equilibrium with ambient air is restored. We have no evidence to suggest that the transient...

5. Improving Transduction Efficiency with Exosome AAVs

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   Blog Post

   ...vehicle for drug delivery that has recently gained interest special contexts, such as the delivery of therapeutic...

6. Learning Technical Skills at a Lab Central Workshop

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   Blog Post

   ...Komuhendo is a Lab Technician at Addgene who is interested in medicine and music.       Additional Resources...

7. Cultivating Community Science at BosLab

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   Blog Post

   ...realized that it was easy to find people who were interested. For example, our journal club discussion on ...

8. Plasmids 101: NGS Quality Control for Pooled Libraries

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   Blog Post

   ...and if you have any tips, input, or stories of interest about library use, let us know in the comments...

9. DAP arrays for multiplex gene editing

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   Blog Post

   ...editing, in virtually any cell type or organisms of interest with only minimal genetic payloads. Different ...

10. Addgene Welcomes John Doench to the Advisory Board

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    Blog Post

    ...drawing me towards the Broad. In order to ask interesting biological questions, you need a tremendous amount...

11. Resume Writing for Non-academic Science Careers

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    Blog Post

    ...browsing job postings and look at the jobs you are interested in. Consider listing the skills you already have...

12. Plasmids 101: Mammalian Vectors

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    Blog Post

    ...assayed conveniently. A direct test for your gene of interest may be another method to assess transfection success...

13. What is Polymerase Chain Reaction (PCR)

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    Protocol

    ... You may also like... Plasmid Cloning by PCR Restriction Digest of Plasmid DNA DNA Ligation Background... DNA: Contains the portion of DNA that we are interested in amplifying for analysis and manipulation. ...

14. Virus Protocol - Generating Stable Cell Lines

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    Protocol

    ...generate stable cell lines expressing a gene of interest from an integrated lentiviral vector....generate stable cell lines expressing a gene of interest from an integrated lentiviral vector. Unlike the...

15. Fluorescent Proteins 101: Luciferases

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    Blog Post

    ...thousands of reporter constructs with a gene of interest already inserted. In addition, we have recently...

16. Luminescent Imaging with Nano-lanterns

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    Blog Post

    ...and photobleaching. Researchers have long been interested in using luminescence to get around these issues...

17. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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    Blog Post

    ...Variants Identifies Oncogenic Alleles. Kim E, Ilic N, Shrestha Y. et al. Cancer Discovery 2016. PubMed PMID: ...

18. Multiplex Genome Editing with CRISPR-Cpf1

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    Blog Post

    ...multi-gene editing with a single crRNA array. Are you interested in using Cpf1 multiplexing in your research? ...

19. Addgene’s Viral Service - Why Virus? Why Now?

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    Blog Post

    ... developments on this project and on all the interesting things going on at Addgene. Sharing speeds science...

20. Sweating the Small Stuff: Details in the Lab

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    Blog Post

    ...experiment.  For example, I used to constantly underestimate time needed to prep for an experiment (labeling...