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...Komuhendo is a Lab Technician at Addgene who is interested in medicine and music.       Additional Resources...

...realized that it was easy to find people who were interested. For example, our journal club discussion on ...

...and if you have any tips, input, or stories of interest about library use, let us know in the comments...

... is opened and equilibrium with ambient air is restored. We have no evidence to suggest that the transient...

...drawing me towards the Broad. In order to ask interesting biological questions, you need a tremendous amount...

...browsing job postings and look at the jobs you are interested in. Consider listing the skills you already have...

...assayed conveniently. A direct test for your gene of interest may be another method to assess transfection success...

...thousands of reporter constructs with a gene of interest already inserted. In addition, we have recently...

...These modifications result in conformationally restricted RNA which improves mismatch discrimination and...

...understanding that AAV-BR1 has the same genome size restrictions as other AAV. Therefore, one should aim to keep...

...experiment.  For example, I used to constantly underestimate time needed to prep for an experiment (labeling...

...protein in an experiment with many proteins of interest! Research the brightness of your fluorophores ...

...primary antibody but doesn’t bind your antigen of interest. Isotype controls are used to make sure the staining...

... binds to a specific region of your protein of interest. For example, if you are studying a protein that...

...gRNA and enrich cells for your CRISPR mutant of interest, give these methods a try.  References Højland...

... ZFN require custom nucleases for each gene of interest. CRISPR-Cas9 tools have been used in immunology...

...mRNAs and two nuclear long non-coding RNAs. One interesting twist to Cas13d enzymes is their cleavage pattern...

...and photobleaching. Researchers have long been interested in using luminescence to get around these issues...

...Variants Identifies Oncogenic Alleles. Kim E, Ilic N, Shrestha Y. et al. Cancer Discovery 2016. PubMed PMID: ...

...multi-gene editing with a single crRNA array. Are you interested in using Cpf1 multiplexing in your research? ...