DAP arrays for multiplex gene editing
Type
Blog Post
...editing, in virtually any cell type or organisms of interest with only minimal genetic payloads. Different ...
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Improving Transduction Efficiency with Exosome AAVs
TypeBlog Post...vehicle for drug delivery that has recently gained interest special contexts, such as the delivery of therapeutic... -
Plasmids 101: NGS Quality Control for Pooled Libraries
TypeBlog Post...and if you have any tips, input, or stories of interest about library use, let us know in the comments... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog Post... is opened and equilibrium with ambient air is restored. We have no evidence to suggest that the transient... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog Post...Komuhendo is a Lab Technician at Addgene who is interested in medicine and music. Additional Resources... -
Cultivating Community Science at BosLab
TypeBlog Post...realized that it was easy to find people who were interested. For example, our journal club discussion on ... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog Post...drawing me towards the Broad. In order to ask interesting biological questions, you need a tremendous amount... -
Resume Writing for Non-academic Science Careers
TypeBlog Post...browsing job postings and look at the jobs you are interested in. Consider listing the skills you already have... -
Plasmids 101: Mammalian Vectors
TypeBlog Post...assayed conveniently. A direct test for your gene of interest may be another method to assess transfection success... -
General Transfection
TypeProtocol...transfect mammalian cells with your plasmid of interest....Transfections allow for transient expression of a gene of interest in a target cell line and can be useful for short... -
Fluorescent Proteins 101: Luciferases
TypeBlog Post...thousands of reporter constructs with a gene of interest already inserted. In addition, we have recently... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog Post...mRNAs and two nuclear long non-coding RNAs. One interesting twist to Cas13d enzymes is their cleavage pattern... -
AAV Q&A with Tim Miles
TypeBlog Post...understanding that AAV-BR1 has the same genome size restrictions as other AAV. Therefore, one should aim to keep... -
Sweating the Small Stuff: Details in the Lab
TypeBlog Post...experiment. For example, I used to constantly underestimate time needed to prep for an experiment (labeling... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog Post...protein in an experiment with many proteins of interest! Research the brightness of your fluorophores ... -
Antibodies 101: Isotypes
TypeBlog Post...primary antibody but doesn’t bind your antigen of interest. Isotype controls are used to make sure the staining... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog Post...ability to reversibly bind and release a protein of interest in response to light allows for tagless protein... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog Post... ZFN require custom nucleases for each gene of interest. CRISPR-Cas9 tools have been used in immunology... -
Luminescent Imaging with Nano-lanterns
TypeBlog Post...and photobleaching. Researchers have long been interested in using luminescence to get around these issues... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog Post...Variants Identifies Oncogenic Alleles. Kim E, Ilic N, Shrestha Y. et al. Cancer Discovery 2016. PubMed PMID: ...