We narrowed to 838 results for: tat
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TypeBlog Post...have a major impact on experimental design and interpretation of results. Take extra care when evaluating... present at both the injection site and in the putative projection neurons. When using this serotype to...
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A Deep Dive into BioBuilder
TypeBlog Post...BioBuilder curriculum is now taught in almost every state in the U.S. and in more than 30 countries around...The BioBuilder Learning Lab is a fully equipped, state-of-the-art lab embedded in LabCentral’s community... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...essentially any gene,” she says. The technical leap from mutating and deactivating a gene or genes to selectively...that the SAM system is robust, specific, and can facilitate genome-scale gain-of-function screening when ... -
3 Challenges in Plant Synthetic Biology
TypeBlog Post...excited to see how this exchange of genetic tools facilitates plant science. Challenge #3: Intellectual property...in 2004, she accepted a commission in the United States Air Force. She left active duty to pursue a degree... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog Post...Assign each meeting a leader from the group in a rotation. The leader for that meeting is responsible for...and meet to debrief on the topic (career) or presentation style (science). Have a potluck dinner. Invite... -
dTAG - You're it!
TypeBlog Post...selective small molecule binders. To overcome this limitation, we created a generalizable tag-based strategy...context. Immunoblotting is recommended, while quantitative proteomics experiments will provide the best... -
RANbodies: Reporter Nanobody Fusions
TypeBlog Post...RANbody platform from the Sanes Lab overcomes this limitation and allows for the flexible design and small ...you own DNA ladders with these plasmids from Penn State Need help picking the right fluorescence microscopy... -
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog Post...and involved presentations and a handout on protocol sharing, material sharing, computational reproducibility... -
Celebrating 15 Years of Scientific Sharing
TypeBlog Post...We are delighted at the early outcomes and the ecstatic reception by scientists. The success of these...candidates from internal referrals. Our long reaching reputation helps us hire great people from farther away ... -
Antibodies 101: Affinity Reagents
TypeBlog Post... in the orientation of the two fragments. However, rigidity can be introduced through mutation, allowing... -
Antibodies 101: Secondary Antibodies
TypeBlog Post...sometimes assumed that the direct method is more quantitative than the indirect method. But in reality, multiple...immunoimaging assays do not allow for absolute quantitative analysis of a sample. Instead, relative quantification... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog Post...therapeutic delivery by AAV, which has strict capacity limitations. New efforts have recently been made to increase...engineering goals expand beyond generating frameshift mutations or single base pair edits, these enzymes are worth... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post...routinely fused to ‘tag’ or ‘label’ proteins to facilitate its detection. Fluorescent proteins are most ...chemogenetic receptor. This may not be an important limitation in many studies, and could even be beneficial... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post...plasmid. Each gRNA combination can be tracked and quantitatively analyzed by sequencing its set of barcodes....: Forward oligo: 5’- CACCGNNNNNNNNNNNNNNNNNNNGTTTGGGTCTTCGAGAAGACCTATTCXXXXXXXXC -3’; Reverse oligo: 5... -
The Future of Research Symposium Boston 2015
TypeBlog Post...at this event added to a growing dialogue on the state of a system that, despite all of its successes, ...Andrew Young School of Policy Studies at Georgia State University, is an expert on the academic labour ... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post...the take rate of mouse xenografts after cell implantation. Their goal was to figure out how to optimize...lung cancer cell line containing an activating mutation in epidermal growth factor receptor (EGFR) with... -
Plasmids 101: Golden Gate Cloning
TypeBlog Post... that flank their DNA fragment in an inverse orientation. This allows for multiple DNA components (promoters...amplification can be used to create silent point mutations at internal recognition site(s) thus eliminating... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog Post...genetic engineering on a genomic scale, with the expectation for major advances in agriculture, materials,...could fix these things, then I think there is no limitation regarding the size of the networks you could ... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog Post...sense to get my master’s and leave. I was really devastated when that happened. I didn't know if I should.... The choice to not go to medical school was devastating. At the same time, she has an entirely new path... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...MuLE (Multiple Lentiviral Expression) system facilitates the simultaneous introduction of multiple genetic...overexpression, knockdown (shRNA, miR-30-shRNA), mutation/editing (CRISPR-Cas9) or deletion (Cre), together...