Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 846 results for: tat

Showing: 601 - 620 of 846 results

  3. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...plasmid of all time, but was found to have a point mutation in the puromycin cassette that makes it less efficient...(BB)-2A-Puro (PX459) V2.0. It contains a point mutation in the puromycin cassette that makes it less efficient...

  4. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...non-functional 𝛽-galactosidase. Complementing this mutation by expressing this deleted portion, for example...custom primers are especially helpful in verifying mutations that wouldn't be reached by sequencing from either...

  5. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...If you missed the webinar, you can watch the presentation and Q&A session on our YouTube channel. And ... a standard curve and somewhat more reliable quantitation. Differences in sample treatment across methods...

  7. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...excited to see how this exchange of genetic tools facilitates plant science. Challenge #3: Intellectual property...in 2004, she accepted a commission in the United States Air Force. She left active duty to pursue a degree...

  9. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...harnessed for stable delivery of various genes, mutations, or treatments into cells, and has been widely...possible development of cancer. Pseudotyping Tropism dictates which types of host cells the gamma-retroviral...

  10. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...therapeutic delivery by AAV, which has strict capacity limitations. New efforts have recently been made to increase...engineering goals expand beyond generating frameshift mutations or single base pair edits, these enzymes are worth...

  11. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...sometimes assumed that the direct method is more quantitative than the indirect method. But in reality, multiple...immunoimaging assays do not allow for absolute quantitative analysis of a sample. Instead, relative quantification...

  13. dTAG - You're it!

    Type
    Blog Post
    ...selective small molecule binders. To overcome this limitation, we created a generalizable tag-based strategy...context. Immunoblotting is recommended, while quantitative proteomics experiments will provide the best...

  14. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...RANbody platform from the Sanes Lab overcomes this limitation and allows for the flexible design and small ...you own DNA ladders with these plasmids from Penn State Need help picking the right fluorescence microscopy...

  16. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...We are delighted at the early outcomes and the ecstatic reception by scientists. The success of these...candidates from internal referrals. Our long reaching reputation helps us hire great people from farther away ...

  19. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...at this event added to a growing dialogue on the state of a system that, despite all of its successes, ...Andrew Young School of Policy Studies at Georgia State University, is an expert on the academic labour ...

  20. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...the take rate of mouse xenografts after cell implantation. Their goal was to figure out how to optimize...lung cancer cell line containing an activating mutation in epidermal growth factor receptor (EGFR) with...
Showing: 601 - 620 of 846 results