We narrowed to 653 results for: EPO
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TypeBlog Post...Hu et al., 2018). In 2020, the Kleinstiver lab reported the development of near-PAMless Cas9 variants....Streptococcus thermophilus (St) NNAGAAW Treponema denticola (Td) NAAAAC ~20 additional Cas9...
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CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog Post...of interest and an inactivated fluorescent RNA reporter. If the target sequence is present in the pool...activity of Cas13a becomes activated and the RNA reporter will be cleaved resulting in activation of the... -
How to Write a Scientific Review Article
TypeBlog Post... member of a class of proteins was mutated, as reported in various studies. We made a table that listed...for each protein we listed all the studies that reported mutations in that protein (including how frequently... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post.... Specifically, efficacy rates of Cas7-11 were reported as up to 85%, broadly similar to shRNA and Cas13...However, in those same comparisons, the AbuGoot lab reported no detectable effect on cell viability – meaning... -
Hot Plasmids Spring 2024
TypeBlog Post... plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles. Here's ...antibodies were recently added to the Addgene repository of tools for SAR-CoV-2 research. Two new antibodies... -
Hot Plasmids - November 2023
TypeBlog Post... plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. Today, it'...or inaccessible. The Ting lab recently made two deposits that increase the utility of this technique. ... -
Viral Production at Addgene
TypeBlog Post...expression and/or function. These data are sometimes reported or posted on the material page for the corresponding...recipient’s initial thaw will be accounted for in our reported titers. Lentiviral vectors are titered using a... -
Viral Vectors 101: Pseudotyping
TypeBlog Post... inactivated by the complement system in serum (DePolo et al., 2000). Other envelope proteins for pseudotyping...–398 . https://doi.org/10.2174/1566523054546224 DePolo NJ, Reed JD, Sheridan PL, Townsend K, Sauter SL... -
An Inside Look into Addgene's Dev Team
TypeBlog Post...tracking plasmids from the moment a deposit kit is sent to a depositor, to streamlining the order fulfillment... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post...over a decade back when Yamanaka and colleagues reported that it is possible to reprogram differentiated... blood, a huge improvement from the previously reported efforts. The pCXLE episomal system thus provides...