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We narrowed to 938 results for: Kin

Showing: 311 - 320 of 938 results

  1. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...Novartis Oncology.  Bhang did her PhD work at Johns Hopkins and came to Novartis for her post-doc in January...after treatment with EGFR inhibitor, erlotinib. Looking at barcode abundances among multiple replicate ...Pharmacology group at Novartis Oncology, Bhang has been working on using the ClonTracer library to monitor clonal...their own work. She says, “I spend a lot of time packing, shipping, and sharing protocols one by one,” and...is a “really great way of broadly sharing these kinds of reagents.” Here at Addgene, we’re excited to ... to use! Many thanks to Carrie and Novartis for taking the time to talk with us and for providing this...

  2. Engaging with science and society at pgEd

    Type
    Blog Post
    ...the hopes and concerns of these communities. This kind of engagement and two-way dialogue is crucial if...advising writers and directors in Hollywood; and working directly with community organizations, libraries...leadership and communication experience. These included taking extra coursework and attending seminars in bioethics...writing science articles for a lay readership, and working with middle and high school students. One of these...organizations was pgEd. After obtaining my PhD and working in digital academic publishing for a year, I’ve...and in helping you decide whether you enjoy this kind of work. It is also beneficial to hone your non-...

  3. Lab Automation at Addgene

    Type
    Blog Post
    ...deposits per month!), manual transformations were taking a lot of time. Enter the Biomek FXP! We wanted ...improve our transformation protocol and, after looking at a few options, we decided on Beckman Coulter...protocol on the Biomek FxP is pretty simple. By clicking a few buttons you can quickly tell the Biomek ...pick up 8 tips and position the tips indepently making it easy to spread out your samples. This can be...scientists share plasmids across the globe, we’re working on ways to send plasmids as DNA as opposed to in...it suits our plasmid prepping needs perfectly. Working with Qiagen, we developed a protocol to run up ...

  4. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    ...proteins had to be re-engineered for each gene, making wide-scale gene activation seem next to impossible...activation uses dCas9, a CRISPR protein variant lacking its endonuclease ability, to bind to genes without...there are multiple options to choose from when looking to activate genes across diverse cell lines.   ...activation compared to other CRISPR activators, making it a popular method for gene activation experiments...design (SunTag). This streamlines its delivery, making it a common choice for CRISPR activation. Newer... Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA (2013) Repurposing CRISPR as an RNA-Guided...

  5. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...started working in the pharmaceuticals industry after earning my Master’s in 2016 and then working four ...prepare 1-2 unique questions for each interviewer by looking at their LinkedIn profiles. This can help spark...your 1-hour presentation to your audience Start by asking the hiring manager or recruiter what presentation...improving a process or procedure, solving a problem, or making a key decision. Even deciding to stop pursuing ...sharing your screen and won’t be able to see who’s asking a question. Maybe you ask for questions after completing...

  6. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ... (and Addgene depositor) Craig Mello. Taking his groundbreaking work in RNAi one step further, his lab...the majority of attendees were scientists still working at the bench. It was a fantastic opportunity to...cell membrane. His recent work shows that they are making fairly remarkable progress on establishing a system...the drug resistantance genes in the bacteria thus making the bacteria sensitive to known antibotics. These... CRISPR resources Read Joanne Kamens' Post on Networking at Scientific Conferences  ...

  7. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ... gRNA using restriction enzyme BsaI. If you’re working in Drosophila, a two-gRNA expressing plasmid is...outside of their recognition sequence, creating flanking overhangs. These overhangs can be customized to...vectors each contain Type IIS restriction sites flanking the promoter-gRNA construct, but with different...with the appropriate Type IIS enzyme, the unique flanking overhang sequences can link together to allow ...constructs with multiple promoter-gRNA cassettes, making them advantageous for small capacity vectors like... mammalian option described below, plasmids for making polycistronic gRNAs are also available from the... tyr gRNA, which causes hypopigmentation, thus marking cells that have undergone genomic modification....

  8. Common Injection Routes in Mice

    Type
    Blog Post
    ...between skin and muscle 25–27 < 2–3 mL (~1 mL per site) Beginner Intradermal Anesthesia Between skin layers...Subcutaneous (SQ or SC) injections are given between the skin and the muscles. The most common injection sites... (ID) injections occur between the layers of the skin. While this could theoretically be performed anywhere...shaved first. Restraint: anesthesia Location: between skin layers Gauge: 26 Volume: < 0.05 ml Skill required...cringe or your experiment will allow you to avoid poking the mice, these “no needles” techniques may come...

  9. Of Myc and Men

    Type
    Blog Post
    ...mentioned above basically involve taking the Myc1-9E10 variable regions and sticking them onto different scaffolds...tagging strategy, another group of researchers was working on isolating monoclonal antibodies to better probe...used in way more applications.   Fig. 1 Making a Protein Tag (Abridged) (1) Locate a promising...same antibody developed almost 40 years ago? Well…kinda. In some cases, antibody providers are distributing.... 1997; Schiweck, et al. 1997) with the goal of making the antibody more amenable to protein engineering...

  10. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ...and feel the satisfaction of checking off each step as you painstakingly complete your experiment – one...Addgene Website: http://www.addgene.org/ If you’re working with plasmids and you decide to share them using...of useful molecular cloning protocols, including making LB agar plates, restriction digest of plasmid DNA.../ Labguru is a good place to go if your lab is looking to streamline your lab management and you want ...reagent storage and inventory, managing orders, tracking students and research projects, electronic lab...
Showing: 311 - 320 of 938 results