...be automatically adjusted every 3 years. In May 2016, Secretary of Labor Thomas Perez updated the overtime...the Addgene Blog How did this change in labor laws come about? In March 2014, the White House issued a...must be above this level, and certain job duty tests must be met. In the Department of Labor’s general... to $47,476 per year, under updates to the Fair Labor Standards Act (FLSA). This is having a major effect...ensuring that either all affected scientists are paid above this threshold or for tracking the hours that these... a memorandum to the Secretary of Labor to update the FLSA, which had not seen revisions since 2004. In... In July 2015, the Department of Labor issued a Notice of Proposed Rulemaking, soliciting feedback by ...

...variants. Klapoetke et al., Nat Methods 2014 Mar 11;(3):338-46. For more information and descriptions of...counterparts. The Janovjak lab has deposited their 3 Opto-RTK constructs – Opto-mFGFR1, Opto-hEGFR, and...turn green. Mashiko et al., Sci Rep 2013 Nov 27; 3:3355. Rinehart lab reagents for improved expression...96-102. Druckmann et al., Neuron. 2014. Feb 5;81(3):629-40.  Next-Gen Brainbow toolkit for neuronal...2012 Mar 20;109(12):E690-7. For further reading about SpyLigase technology, read the Addgene interview...; DOI: 10.1021/sb4001504. For further reading about Nicola Patron's MoClo kit and her plant synbio research...in optimizing TALEN assembly and activity? The laboratory of Takashi Yamamoto has created a complete TALEN...

...Tris-acetate 40–500 kDa Tris-tricine 150 V, 1–3 hours Good for higher weight proteins Long running...very large proteins. A semi-dry transfer is faster — 3–30 minutes — and uses less buffer, but it is not as...approaches best suit your experimental needs. Table 3: Optimizing the technical design of a western blot...If you’ve ever run a western blot, or thought about running one, you’ll know there’s a lot of choices...

...cyanobacterium Vampirovibrio chlorellavorus." PeerJ 3 (2015): e968. PubMed PMID: 26038723. PubMed Central... Additional resources on the Addgene blog Read about company culture at Addgene See some of our previous...

...for more on timely submission of a job application. 3) Read job descriptions carefully Be open minded and... your strengths in your resume and don't be shy about asking for help. The worst thing that can happen...

...to wash Eppendorf tubes which were to be used for 3 months before they could be discarded.  Seeding Labs...Reddit AMA  Few of us spend much time thinking about conserving resources in the labs. Disposable plastic...Dudnik, Founder of Seeding Labs, tells the story about working as a student in a rice lab in the Ivory ...didn’t feel the need to reuse plasticware or think about conserving reagents and buffers.  However, I had...environmentally conscious friends who had me thinking about energy conservation even before it was a popular...Turns out, one could make a lifetime supply for about $10 US dollars worth of glass powder pruchased from...So, I designed my own gel box (see illustration above) and bribed the guys in the hospital machine shop...

...it doesn’t help much if the actual response takes 3 days and still doesn’t even answer your question. ...not even get a solution to the problem you called about. Next, you try an email, given that this is the ...” next to an encircled question mark (see image above). This useful widget allows you to access our Help... or you just have a general question or concern about the answers we provide, within the widget you also...Addgene Blog Read other Inside Addgene Posts Learn about New Features on Our Plasmid Pages Resources at ...

...plasmid, which contain regions of dsDNA. To generate a 3’ hydroxyl group needed for DNA replication, Rep78/...engineered Learn about the parts of the AAV transfer plasmid  Resources on Addgene.org Learn about AAV Find ...plasmid! Check out this blog post to learn more about the AAV transfer plasmid! References: Naso MF...

...C-terminus (3xHA, 13xMyc, GST, or GFP). Longtine et al.(3) describe a complimentary set of plasmids for use ...strain. In addition to the collections featured above, many other modular yeast tagging systems have been...Addgene would love to hear from you, our community, about your experience with yeast genome modifications....

... ways we get your plasmids out there Beyond these 3 big pushes, we try to increase your work’s visibility... will be able to easily find the fruits of your labor and won’t have to repeat the modification. This ...re ever interested in writing your own blog post about a plasmid or set of plasmids you’ve deposited with...