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We narrowed to 993 results for: Ran

Showing: 621 - 640 of 993 results

  1. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...technical support Our customer and technical service brands continue to be impeccable. We score ~99.9% helpful...lauded by another set of stakeholders - the tech transfer community. In a recent law review article we received... have a UI/UX team. Last time I checked we were ranked as the 21,806th most popular website in the world...pages and excellent work on our external website appearance and functionality are happening and this strengthens...

  2. Summer SciComm: Preprints

    Type
    Blog Post
    ...conference poster or talk, or submitting patent or grant applications; to informal settings like lab meetings..., and increase their impact. Open access and transparency Another major benefit of preprints is their ... of listing “Submitted” articles in their CV or grant applications. A number of organizations (such as...be prone to biases or errors, with a lack of transparency in most review processes. Preprints and their...

  3. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...Gate cloning, perform an exonuclease cleanup, transform, plate, and pick. In short, the Fragmid system...AAV pRDA_889 (ITRs from AAV2) Mammalian Transposon pRDA_757 (Piggybac; constitutive expression...   Drosophilia Plasmid pRDB_053 (for transgenic flies; gypsy insulator sequences; white + selectable... drosophila) Delivery type (lentivirus, AAV, transposon, Plasmid) Destination vector (dropdown options...

  4. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    ...expression in E. coli. This operon is under the transcriptional control of a strong constitutive promoter (... required enzymes, including a glutathione S-transferase homolog from a Janthinobacterium sp. Moreover... protein kinase A (PKA) activity at the plasma membrane in living cells using a construct in which TagRFP-T...used in quenching, FRET (Förster resonance energy transfer), and FLIM (fluorescence lifetime imaging microscopy...

  5. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    ...chain and light chain for the antibody and when translated into protein will assemble into a fully functional...synthesize the genes that encode those amino acids (Tran et al., 2016). Each antibody would then be tested...P, Frenzel A, Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018) When monoclonal antibodies...:633–644 . https://doi.org/10.1083/jcb.201409074 Tran NH, Rahman MZ, He L, Xin L, Shan B, Li M (2016) ...

  6. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    ...fall within a 10 fold range. Plasmids that don't meet the lower bound of this range are “failed” and removed...mutations, we check to see if they affect the translated amino acid. We also confirm that the species ...2017) Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening. Nat Protoc 12:828–863...

  7. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...fragments are named according to the number of strands in the canonical beta-barrel structure. Most frequently...frequently, the proteins are split between strand 10 and 11 to yield large (1-10) and small (11) fragments...oligomerisation. Eastwood T, Baker K, Brooker H, Frank S, Mulvihill DP. FEBS Lett. 2017 Dan Mulvihill ...shifting tag (FAST) for use with green-yellow or orange-red fluorogen A split fluorescent reporter with...

  8. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right: T1 tomato plants that inherited an edited...Cultured rat hippocampal neuron expressing pan-membrane ASAP5. Solid blue circle marks the initiation ...via the Kv2.1 targeting motif as well as a pan-membrane construct without the Kv motif. Altogether, if...kinetics and high responsivity near the resting membrane potential, ASAP5 is a great new option. Find ASAP5...

  9. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    ...would at least be guaranteed a successful result? While there are never any guarantees, some cloning techniques...Resources” section at the end of the post! Speed: Your grant deadline is approaching or your advisor is demanding...your insert, DpnI digest away the templates, and transform the PCR products into E. coli. Ligases within ...

  10. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...production of dsRed E5. This timer predictably transitions from green to red fluorescence (500 to 580 nm... of vesicle fusion and release from the plasma membrane by fusing various vesicle cargoes to FP timer ...fusing Medium-FT to LAMP-2A, a lysosome-associated membrane protein, and following its subcellular localization...whether or not newly synthesized or long-lived transcription factors control gross changes in gene expression...

  11. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...sequences that, when bound by transcription factors, can increase a gene’s transcription. Sometimes enhancers ...and proteins that forms chromosomes. These long range DNA interactions are often detected with chromosome...control gRNA or predicted gRNA off-target sites. Long-range chromatin interactions identified with the multiplexed...

  12. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    ...during ESC isolation. Low Chances of post-transplantation rejection when used therapeutically High Low...similar functional utility, iPSCs bypass the transplantation compatibility issues and ethical concerns of... used therapeutically, the chances of post-transplantation rejection are low. Further, as iPSC generation...somatic cell type to another, also known as transdifferentiation.   Many thanks to our guest blogger, Kusumika...

  13. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...blastn), Standard Protein BLAST (blastp), and Translated BLAST (blastx). NCBI has a terrific getting-started...gaps in the nucleotide sequence, you can run a Translated BLAST (blastx) search to check for expected ORFs..., due to the additional searches involved in translating the nucleotide sequence into all six possible...choose for your sequencing result and can easily translate it, we recommend using blastp over blastx. The...

  14. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...cases being reported (right, red), likely due to quarantining {% video_player "embed_player" overrideable...-examine our global data at a finer temporal granularity. For each paper, we observed that the demand ...imposed on scientists. Figure 6: Model fit. Orange line depicts observed data. Blue line is the sum...depending on when we believe the China COVID-19 date range is, they could be the most impacted or least impacted...

  15. MXS Chaining

    Type
    Blog Post
    ...visualize cell cycle dynamics, titrate inducible transgene expression, or a variety of other clever applications...expression) and a polyA signal (to terminate transcription) and the four constructs were then combined ...derived from the tyrosine-protein kinase Lyn) Membranes 3 mCherry 587nm/ 610 nm human β-Actin Actin ...their 6-mer sequences, are rarely found in the transcriptomes of these species and can often be used to clone...

  16. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...expressing neurons and GFP (green) to label neurons transduced by each serotype. Working at Addgene has ...answers hundreds of AAV questions every year on a range of issues related to AAV vectors, many from people...S., & Klein, R. L. (2018). More expansive gene transfer to the rat CNS: AAV PHP.EB vector dose–response.../10.1038/s41434-018-0028-5 de Backer, M. W. A., Brans, M. A. D., Luijendijk, M. C., Garner, K. M., & Adan...

  17. CRISPR Antimicrobials

    Type
    Blog Post
    ...antibiotic resistance using multiple approaches. Major restaurant chains including Chipotle and Panera Bread have... joining machinery, so a CRISPR-induced double-stranded break (DSB) in the genome is lethal. If this DSB...diverse, and each phage has a narrow bacterial host range. To further potential clinical applications, Ando...Beisel, Chase L., Ahmed A. Gomaa, and Rodolphe Barrangou. “A CRISPR design for next-generation antimicrobials...

  18. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ... elaborately pranking each other, dressing in costume, and holding competitions (ranging from cook-offs...orderly and timely fashion. 9. Ask for help My grandma used the old adage “many hands make light work”..., evite, posters, megaphone, or your company’s intranet. Don’t forget about the power of simple word of...

  19. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ... package stays in transit for a certain number of days and tracking average transit time for each courier... often weren’t easily accessible, due to a wide range of logistical barriers. Addgene worked to identify...Protocols Agarose Gel Electrophoresis Bacterial Transformation How to Design a Primer Creating Bacterial Glycerol...

  20. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ... protein of interest, such as a sample transiently transfected or stably infected with the protein of ...downregulated, examine a protein’s stability or post-translational modifications, or study how a target protein...experiments using a tagged protein may not be translatable to the endogenous system and will need to be...
Showing: 621 - 640 of 993 results