We narrowed to 762 results for: cell

...force to control protein expression in mammalian cells: easy to use biological tools are abundant! In this...tag prior to introduction of the construct into cells. However, if you would like tunable control of an...

... channels in living cells. Multiple properties of a channelrhodopsin, such as cell localization, kinetics...

...strain, the promoter being used should be tested in cell lines to determine how it impacts the expression...interest. Cre may be expressed globally (in every cell of the mouse) or in a tissue-specific manner (with...

...applications like recombinant protein purification and cell-free circuit design in E. coli - Freemont Lab MoClo-YTK...

...annealing to a complementary ssDNA target in the cell.  Only Beta expression is required for recombineering...definitely the easier of the two options, but these cells are prone to mutations and will have more unintended.../Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR Methods for Bacterial Genome Engineering ...

...allow scientists to express a gene of interest in a cell or animal model. AAV plasmids contain several important...Integrity on rAAV8 Production Using the Baculovirus/Sf9 Cells System. Hum Gene Ther Methods, 28(5), 277–289. https...

...Different AAV serotypes have preferences for specific cell types. For example, AAV1 and AAV9 serotypes have...most cases with VSV-G that generally mediates broad cell tropism and has a high percentage of neuronal targeting...

...hypothesized that life started with RNA and a simple cell membrane. His recent work shows that they are making... circular RNA they have identified resulted in a cell migration defect. They have created a circRNA website...

...protein-coupled vertebrate rhodopsin in cerebellar Purkinje cells. The Journal of biological chemistry. 2011;286(29...Programmable In Vivo Pharmacology and Optogenetics. Cell 162.3 (2015): 662-674. doi: 10.1016/j.cell.2015.06.058...

...and is able to target and edit RNA in mammalian cells. Type IV Type IV is the middle child (like me!) ...-Cas3 for genome-editing applications. Molecular Cell, 84(3), 463-475.e5. https://doi.org/10.1016/j.molcel...RNA Cleavage during Type III CRISPR-Cas Immunity. Cell, 161(5), 1164–1174. https://doi.org/10.1016/j.cell...biotechnology toolbox for and beyond genome editing. Cell & Bioscience, 8(1). https://doi.org/10.1186/s13578...

...concepts and challenges. Molecular Biology of the Cell , 30 (11), 1249–1271. PMID: 31084567 (Link opens...

...and the resulting heteroduplex is resolved by the cell’s mismatch repair (MMR) system. Alternatively, if...

.... Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Sander JD, Cade L, Khayter...

... tools that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments...

...conversation! Do you have a question about culturing iPS cells? Confused by which system to use for genome editing...

...different substances can be injected, including cells, drugs, DNA, viral vectors, and viruses. All researchers... injections are most often used when injecting a cell suspension to create xenograft models. Restraint...

...the African American woman whose cervical cancer cells became a mainstain of biological study without her...

... chemically induce neuronal activity in specific cell types. See our Chemogenetics Plasmids and Chemogenetics...

...Your Favorite Fluorescent Protein Oligomerizes in Cells   ...and many more! Sign up to write for the Addgene...

...screening for functional long noncoding RNAs in human cells by Cas9 targeting of splice sites. Nat Biotechnol...non-coding RNAs with the CRISPR/Cas9 system in human cell lines. Nucleic Acids Research. 2014;43(3):e17. doi...