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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,041 results for: ins

Showing: 621 - 640 of 1041 results

  1. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    ...the page looked clean, professional, and modern. Instead of trying to place all of the content above the... regularly be paired with a variety of science-inspired friends. As you can see on the homepage, Blugene... you can find what you need and feel happy and inspired to tackle your next science challenge. If you ...

  2. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...plant species. Thus, binary vectors contain two origins of replication, one each for E. coli and Agrobacterium...promoter has been modified with duplicated enhancer domains and characterized in studies of tobacco, Arabidopsis...regions permit multiple regulatory elements to be inserted, further customizing gene expression. Sahoo et...

  3. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...audience to use their useful energy and to choose institutions with allies engaged in training excellence to...experience and encouragement.  Dr. Sibby Anderson-Thompkins, Director of the Office of Postdoctoral Affairs... training programs (like at the Keck Graduate Institute), funding for training (such as the NIH BEST award...

  4. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...systems. As a part of this effort, members of the Rawlins Lab have compiled a new toolkit for performing ...SL fluorescent biosensors by integrating a SL-insensitive fluorophore (LSSmOrange) as an internal control...in tobacco protoplasts, the enzymatic biosensor retains its catalytic activity.  As SLs are also known ...

  5. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...activation to behaviors. Scientists continue to engineer opsins to operate at increasingly faster kinetics. The...by working with organisms with light accessible brains, such as zebrafish larvae. Mouse studies, however...permanent intracranial implant to be surgically installed to allow for light delivery. Chemogenetics poses...

  6. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...favoring the separation of the two helixes. Low pH instead results in the breakage of the ester bonds responsible...the gene endA. Nowadays, the most common E. coli strains used for plasmid amplification are endA negative...mutated to neutralize the nuclease activity. For strains that still carry this gene, there are usually extra...

  8. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ...bacteria. Until recently the only available Cas9 proteins were isolated from mesophilic (medium heat loving... as Streptococcus pyogene’s SpCas9. These Cas9 proteins don’t work well at high temperatures, so to use...when incubated in diluted human plasma. GeoCas9 remains active after being incubated in up to 30% human...

  9. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    ...calculators These links can help you calculate how much insert and backbone you need for different types of cloning...method. You can select from five different insert:backbone ratios. In-Fusion cloning molar ratio calculators...IHC.  FPbase: A data repository for fluorescent proteins and their properties.  Expasy: Bioinformatics ...

  10. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ...knowledge base extensively, you open doors to fresh insights and broaden your understanding, ultimately enhancing...and connections that emerge during moments of inspiration. Additionally, journaling in the morning has ..., whether within academia or industry. Albert Einstein once said: “There are only two ways to live your...

  11. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...were excited when Amos Bairoch from the Swiss Institute of Bioinformatics reached out to us to tell us...surprising, and important, findings being shared. For instance, recently Cellosaurus received a report that HT...out the Cellosaurus FAQ! References Lorsch JR, Collins FS, Lippincott-Schwartz J. Cell biology. Fixing...

  13. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...this is a much-needed community resource. Instead of painstakingly searching for experimental details in ...serotype, promoter, or tool type and each entry contains an image along with key experimental details (...

  14. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...use two antibodies - one against your target of interest and a second against the first antibody. When.... (C) A third solution is to use Fab fragments against the sample species to block the binding sites on...

  15. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ... to carry out a yeast-2-hybrid library screen; instead I thought they would focus on “Has this person ...the Harvard Business Review or some Chartered Institute of Marketing reviews, I would have struggled. ...interviewed for 1 hour so having a broad enough insight was going to help me push both my scientific and...

  16. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ...platform to help researchers working on COVID-19. Its origins trace back to March 2020, when the first coronavirus...use this potential to make a difference not just against COVID-19, but also in other areas of scientific...Hernández-Sánchez is a postdoctoral researcher at Instituto de Investigación Biomédica de Salamanca (IBSAL...

  17. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ... of cell culture medium, which is buffered and contains the pH indicator phenol red. We froze samples ...der Geer P, Manning MC, Fitchmun MI. Protein instability following transport or storage on dry ice. Nat...10(4):278-9. PubMed PMID: 23538862. 2. Potter M, Lins B, Mietzsch M, Heilbronn R, Van Vliet K, Chipman...

  18. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...was robust, capable of generating a wide range of insert sizes and also produced high yields of quality ... an NEB drawstring backpack and a double wall insulated glass to take home with us. Tips to to get the...being overwhelmed by all the new information, and instead allow me to pay attention and answer my key questions...

  19. Cultivating Community Science at BosLab

    Type
    Blog Post
    ... became our bacterial transformation guru and instructor for our intro to biohacking courses. Our members...initiated a soil microbiome project. This project was inspired by previous city-wide genomic studies and our ...DIY bio If you’re not in the Boston area but are inspired to get involved, check out the local groups page...

  20. Components of CRISPR/Cas9

    Type
    Blog Post
    ...DSB) Cas9 and its variants have two endonuclease domains: the n-terminal RuvC-like nuclease domain and the...conformational change that positions the nuclease domains to cleave opposite strands of the target DNA. Thus...refer to this RNA complex as the “gRNA”. The gRNA contains both the 20 nucleotide target sequence to direct...
Showing: 621 - 640 of 1041 results