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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 843 results for: ANG

Showing: 621 - 640 of 843 results

  3. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...diverse needs of our community and support a wide range of experimental designs. Here are nine great additions...

  5. Editor's Choice, July 2016

    Type
    Blog Post
    ...the entire blog and hosted new posts on topics ranging from new tools you can use to monitor cellular ...

  8. A Scientist's Guide to Ebola

    Type
    Blog Post
    ...through the air and that’s considered unlikely to change – but the illness has been fatal in 70 percent ...

  11. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ...this process. We take pride in facilitating the exchange of not just the physical samples, but also the...

  14. Reflections and Looking Ahead

    Type
    Blog Post
    ...Content Accessibility Guidelines (WCAG). These changes mean our content is both available and accessible...

  16. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...sequences from step B.1 into the oligos below. Do not change the ends; these bases are important for cloning...CO 2 for 12-15 hours. Day 3: j. In the morning, change the media to remove the transfection reagent. Replace...Lentiviral particles can efficiently infect a broad range of cell types, including both dividing and non-dividing...to cells. Days 3+: e. Examine cells each day and change to fresh puromycin-containing media every other...Target cells should be approximately 70% confluent. Change to fresh culture media containing 8 μg/mL polybrene...particles per cell. Addgene recommends that you test a range of MOIs to determine the optimal MOI for infection...Incubate cells at 37°C, 5% CO 2 overnight. Day 3: e. Change to fresh media 24 hours after infection. TIP: If...

  17. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...determine when the desired color change has occurred. The color will change from yellow to blue and generally...experimental conditions. When the desired color change has occurred, gently remove the plate seal. Using...generate a standard curve . If you don’t see any color change or dose response, your antibody concentration may...case, use lower antibody concentrations. The ideal range of the standard curve will vary between targets ...your unknown sample’s absorbance falls above the range of the standard curve you will need to either include...

  20. Science as Art

    Type
    Blog Post
    ...Beautiful microscope images which traditionally languished in the pages of thick textbooks are now liberated...
Showing: 621 - 640 of 843 results