...choose? Which way is the right way?  First of all, I want to put to rest a common misconception. I find personal...natural - if it works so well for you, of course you want to share it with others! After all, won't it work... to where your audience already is.  You’ll also want to keep in mind that access and availability to ...resources do you have?  No matter the project, I can guarantee you that either resource or time restrictions ... design a hands-on interactive activity using elephants than it is to design one demonstrating mitosis...Consider also the amount and type of information you want to convey. Small tidbits and fast/fun facts are ...

... likely seem important for any virus, we will see that they are very central determinants of how AdVs ...toward saying that host-virus interactions are an important part of the biology of adenoviruses (AdVs). AdVs...from the genomic plasmid can be used to house a recombinant sequence (transgene) instead.    Although the...natural AdVs, replication-competent viruses with a recombinant genome, and adenoviral vectors all have a genomic...basic research, like other viral vectors, though significant challenges remain (Wold & Toth, 2013).  While...

...the cells’ germline genetic variations. They then wanted to identify cancer-associated genetic dependencies...ancestry, Sean and Aaron knew that their study had significant statistical limitations, and expected to find...Cas9 from cutting, both of which can introduce significant bias into the results. But the reference genome...design of the experiment or dataset itself. It is important for scientists to not only be aware of the potential...ancestral bias and the human genome Wang, T., Antonacci-Fulton, L., Howe, K. et al. The Human Pangenome...doi.org/10.1038/s41586-022-04601-8 Lewis, Anna CF, Santiago J. Molina, Paul S. Appelbaum, Bege Dauda, Anna...

...). Transformed bacteria are then grown up on antibiotics such that only bacteria with the plasmid can ...filtered pipet tips and prepare fresh media with antibiotics at each stage. The barcodes and LIMS help us ...enter the cells. Some samples require a reduced antibiotic concentration or need to be transformed into ...tubes. 1D barcodes are better for tubes that are meant to be read one at a time. 1D barcodes can be purchased...misread.  2. Track the container - It is just as important to track the location of the container housing...to fulfill orders.  The barcoded tubes help to guarantee that no samples get lost during their journey....

...systems. Can you describe this technology and its advantages? Fujii: In the last several years, my lab has...is being involved in the scientific community important to you and your lab? Fujii: I first knew Addgene...considering the peer-review system of journals and grant applications, for example. However, at the same ...interact with each other, which is critically important, especially for young researchers. It was a really...living for Japanese, which is not trivial, and the grant situation is currently much better than that in ...tough competition for academic positions and poor grant situation. So, young researchers need to publish...

...between the purine bases (adenine, guanine, and hypoxanthine) or between the pyrimidine bases (cytosine, ... purine to a pyrimidine or vice-versa. Note: hypoxanthine (shown) is the nucleobase component of the nucleoside...nucleoside inosine consists of the nucleobase hypoxanthine + a ribose sugar. Their fusion of wild-type ...glycosylase / alkyladenine DNA glycosylase  Removes hypoxanthine from DNA, creating an abasic site. The same ...one name. Indel Insertion/deletion A type of unwanted DNA edit. AYBE, AXBE, ACBE Adenine transversion...models-assisted optimization of a uracil-N-glycosylase variant enables programmable T-to-G and T-to-C base editing...

... L., Martínez-Antonio, A., Quiñones-Valles, C., Espíndola-Serna, L., & Martínez-Antonio, A. (2011). Mechanisms...base pairs for the protein to be translated. An antisense RNA called Inc, also encoded by the ColIb-P9 plasmid... the host with a beneficial function (usually antibiotic resistance).  In the lab, low copy plasmids may... competition between pseudoknot formation and antisense RNA binding at a specific RNA site. The EMBO Journal...Leonard, A. C. (2018). Origin recognition is the predominant role for DnaA-ATP in initiation of chromosome...

...recipe. It’s unrealistic to plant, grow, and harvest grains every time you want to bake a cake. Instead, ...development happening at Addgene under the hood. A significant portion of our development may not be visible...changing needs of the teams at Addgene. Another advantage of Scrum is that it provides transparency to everyone... been updated in several years) or they make significant changes that make it incompatible with our software...software from Python 2.7 to Python 3.5. There are significant changes to the language and it took us a total...

...targets for complement-based killing by anti-alphaGal antibodies. The VSV-G envelope protein is inactivated...different viral membranes, tropism, and advantages and disadvantages: Table 1 (Joglekar et al., 2017)  Table...its own, it needs to be pseudotyped. This takes advantage of the EnvA-TVA receptor-envelope system (Wickersham...2006) Retrograde neuronal tracing with a deletion-mutant rabies virus. Nat Methods 4:47–49 . https://doi.org...

...speed easier and faster when you’re an active participant in the training. In this blog we will review ...valuable lessons along the way. We think it’s so important that we have written plenty of articles on building...bench mentor-mentee relationship is extremely important – without a good set up it could be very difficult.... Preparation before bench work Are there any relevant papers you can read prior to the start of your...through the protocol mentally (or physically where relevant) and identify any gaps or questions you have, ...everything is magically made up by helpful TAs or assistants, and sitting out on the bench waiting for you...

... maybe you’re researching a topic for which no relevant or recent review exists. Once you pick a topic...format also has the benefit of exposing you to relevant researchers in the field. Finally, you can sound...from start to finish. Find the information that’s relevant to you, note it, and move on. At the beginning...retreat to the woods for months at a time, so take advantage of whatever time you have. If you’re like me, ...were, and you could find the original paper if you wanted to learn more. Another example: we made a schematic...Tips for Improving Your Next Manuscript 6 Tips for Grant Writing Resources on Addgene.org Check out Our...

...Biddle, of Only Good Antibodies, who shared their antibody validation expertise. Antibody Validation for Flow... Caitlin Faulds, who introduced the very first antibody deposit (the Integrin collection) from the Institute...Institute for Protein Innovation. An Integrin Antibody Toolkit from IPI Simon Moe, from Promega, who ...

...and spread over the second quadrant. The process continues until all quadrants are used, hopefully revealing...plate, first the bacteria are inoculated over a quadrant of an agar plate. Then, a stick is dragged through...revealing single, isolated colonies in the last quadrant after incubation. The challenge is usually successful...characterizing the microbes from the termite hindgut. Want to level up your or your class’s streak plate skills...

...includes small epitopes fused to each XFP. Secondary antibodies that target these epitopes and fluoresce in the...group developed Zebrabow as a system that takes advantage of the multicolor labeling possible from Brainbow...developed from the Brainbow 1.0-L construct, a variant of Brainbow 1.0 that contains RFP, CFP, and YFP...fluorescent neuron is visible. Chemical tags are more resistant to tissue clearing methods and can be labeled ...Corinna, et al. "Mouse cutaneous melanoma induced by mutant BRaf arises from expansion and dedifferentiation..., Alexander, et al. "Visualizing the Fate of Transplanted K14-Confetti Corneal Epithelia in a Mouse Model...

...collaborates with NIH-funded researchers and utilizes grants to support our own initiatives, Addgene is deeply...constrained. This collaborative approach eliminates redundant work, saving time and allowing precious research...DNA sequencing, viral vector preparations, and antibody production that labs may find increasingly difficult...services can be listed as direct costs from a CRO in grant applications, addressing the new funding structures...scientific community for the benefit of everyone. We Want to Hear from You We invite researchers to share ...

...Dr. Koeris' reasons for wanting to develop bacteriophage for use as antibiotics 8:26 - 10:30 : How Dr. ...MIT) where he worked on understanding bacterial antibiotic resistance. During this time, Dr. Koeris and ...bacteriophage (viruses that infect bacteria) for use as antibiotics. To develop this idea, Drs. Koeris and Lu founded...36:53 - 38:00 : As an entrepreneur, is it more important to develop something that could succeed in the...

...been using Zoom’s default backgrounds all year and want a change? Addgene has you covered. Today we’re releasing...hanging out with Aavery in this Zoom video background. Want the purple bacteria video background with mascots...with AAV or lentivirus in your research, you might want to use one of our virus backgrounds. Addgene’s viral...red COVID-19 background.         If you’re predominantly a plasmid user, instead, don’t fret; we have...        Did you get the joke? If not, you might want to check out Addgene’s Fluorescent Proteins blog...

...ready-to-use recombinant antibodies collection, currently composed of over 100 antibodies. Everything ...which includes both neuroscience and general-use antibodies, is available in both trial- and full-size, with...validation data, sharing what works and, equally importantly, what doesn’t. Reports receive a DOI for citation...

...system to developing an antibiotic that selectively kills only the bacteria you want it to, leaving all those...there's a pretty good reason for that. Lately, significant advances in the understanding and application...mutations, both in mice and in human cells. But that important advance was quickly followed by even splashier...All this, and scientists are still working out important details in their understanding of how CRISPR/Cas9s...

...selection (ex: antibiotic selection and auxotrophy) and negative selection (ex: toxin-antitoxin systems and...posts from the year. 10. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators...Photoswitchable Proteins by Cary Valley, Shreya Vedantam, Michelle Cronin, and Angela Abitua Thanks to...us at [email protected].  Thank you again for a fantastic year and happy reading. Best wishes on your research...