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We narrowed to 18 results for: ANT

Showing: 1 - 18 of 18 results

  1. Antibody Guide

    Type
    Guide
    ... are called recombinant antibodies. Recombinant antibodies are created by cloning antibody genes from ...Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection Jump To: Antibody Guide Production... produce antibodies, with the highest variability in antibodies produced. Cells - Antibodies can also ...amounts of a single antibody with little variation. Recombinant plasmids - Antibodies can be produced from...variable region and constant regions of the antibody to be independently manipulated. Antibodies produced this... in a buffer. Antibody Structure Figure 1: Structure of an antibody A standard antibody is made up of ...producing antibodies, can change the constant region, and therefore change the isotype of antibodies they ...

  2. Modular Cloning Guide

    Type
    Guide
    ... MoClo Plant Parts Kit Plant Expression Nicola Patron 95 standardized parts specific for plant synthetic...multi-gene constructs for Xanthobacter or Roseixanthobacter species. LbPlant Toolbox Kits Bacterial Expression...synthetic biology. MoClo Plant Parts II and Infrastructure Kit Plant Expression Johannes Stuttmann 95 additional...plasmids for hierarchical assembly of plant multigene constructs. MoClo Plant Parts III: Transformation & Genome...GreenGate Cloning System Plant Expression Jan Lohmann 56 plasmids to create plant expression vectors containing...generate multi-construct transgenic plants. GreenGate 2.0 Toolkit Plant Expression Alexis Maizel 68 plasmids... multi-gene constructs for plant transformation. GoldenBraid 2.0 Kit Plant Expression, CRISPR Diego Orzaez...

  3. CRISPR Guide

    Type
    Guide
    ...binding) SpCas9 VRER variant 3' NGCG SpCas9 EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9...LbCpf1) 5' TTTV AsCpf1 RR variant 5' TYCV LbCpf1 RR variant 5' TYCV AsCpf1 RVR variant 5' TATV Campylobacter...PAM sequence often found in plants, expanding the targeting range for plant genome engineering. Read more...more about Cas9 variants . Anti-CRISPR Prolonged Cas9 activity can increase the risk of off-target cutting...like 3xFLAG-tag, PA, and biotin tags, or an anti-Cas9 antibody. The CAPTURE system, developed by the Jian...Species/Variant of Cas9 PAM Sequence* Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant 3' ...Journal . 2 (6), 376–394. PMID: 31742433 Durrant, M. G., Fanton, A., Tycko, J., Hinks, M., Chandrasekaran...

  4. Molecular Biology Reference

    Type
    Guide
    ...information on antibiotic resistance and additional antibiotics, see our blog post on antibiotic resistance... grow in or on media containing that antibiotic. These antibiotic resistance genes not only give the scientist... multiple generations. It is important to distinguish that the antibiotic resistance gene is under the...cultures with antibiotics, see Addgene’s protocol on inoculating bacterial cultures . Antibiotic Recommended...Origins of Molecular Genetics The Genetic Code Recombinant DNA Working with Plasmids Plasmid Elements Types...Molecular Cloning Common E. coli Strains Common Antibiotics DNA Sequencing Sanger Sequencing Next-generation...Additional Resources Plasmids are one of the most important tools in molecular biology. They allow scientists...

  5. Optogenetics Guide

    Type
    Guide
    ... its variants allow for inhibition of neurons using blue-green light. Mac Variants. Mac variants have ...wild-type opsin of each and some examples of relevant variants. This list is not exhaustive - please browse...Inhibitory (Hyperpolarizing) ChR Variants. Alternatively, ChR variants that inhibit neurons have been created... these variants result in the hyperpolarization of neurons. Examples of anion channel variants from other...thus inhibiting function of the neuron. NpHR Variants. Variants have been engineered with enhancements such... triggered by green-yellow light. Arch Variants. Arch variants have been developed with the following ...plasmids . Glossary of Microbial Opsin Variants Opsin Type Variant Description Peak Response Spectra (nm...

  6. Adenovirus Guide

    Type
    Guide
    ...in a higher background of non-recombinant adenoviral plasmids. Recombinant adenoviral plasmids are then...Treatment Recombinant adenoviral vectors are also being used in cancer therapy to stimulate anti-tumor immune...specialized immune cells and antibody production that develops after exposure to an antigen. AdEasier-1 cells BJ5183...vectors for recombinant adenoviral vector production that do not contain GFP. Recombinant adenoviral vector...Parvoviridae family. AAVs were discovered as a contaminant of adenovirus preparations, which is how they...information about AAVs, read our AAV guide . Recombinant Adenoviral Vectors Wild-type adenoviruses have...have been modified by researchers to create recombinant adenoviral (rAdV) vectors in order to deliver genetic...

  7. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...Guide . Recombinant AAV Vectors Wild-type AAVs have been modified by researchers to create recombinant adeno-associated...activation, both key advantages of rAAV vectors. AAV Types Serotypes AAV serotypes are AAV variants that differ...Parvoviridae family. AAVs were discovered as a contaminant of adenovirus preparations, which is how they...in order to deliver genetic cargo into cells. Recombinant AAV constructs are designed by replacing the ...Gateway cassette containing the ccdB gene, a ccdB-resistant strain is necessary. For more information on cloning...Created with BioRender.com. Genome Integration Recombinant AAVs are generally considered to not integrate...vectors for in vivo applications. Other Engineered Variants Hybrid capsids Hybrid capsids are engineered capsids...

  8. Molecular Cloning Techniques

    Type
    Guide
    ... is important to confirm that there are no additional sites present within the fragments you want to assemble...Resources Molecular cloning, or the creation of recombinant DNA, is an essential process used in scientific... popular cloning methods for the creation of recombinant DNA. Restriction Enzyme Cloning Restriction enzyme... and ligated into your desired plasmid. It is important to note that restriction enzyme target sites can... fresh PCR product into a plasmid. The major disadvantage of TOPO cloning is that very few plasmid backbones...more commonly known as Gibson assembly, takes advantage of the properties of three common molecular biology...orientation at a time, and without the need for any unwanted sequence at the junctions (such as a restriction...

  9. Plan Your Experiment

    Type
    Guide
    ...(knockout)? Generate a specific mutant allele of a gene (point mutant)? Increase or decrease expression...of a selectable marker (antibiotic resistance or fluorophore) are also relevant. Delivery Using Plasmids... specific disease or process of interest. Do you want to: Generate complete and permanent loss of gene... window from the PAM sequence, and base editor variants often have different optimal editing windows. ...testing multiple pegRNA designs is particularly important for prime editing. CRISPR Intereference : Target...Predicted On-Target and Off-Target Activity Another important factor to keep in mind when designing your gRNA...In addition to off-target activity, it is also important to consider factors that maximize cleavage of ...

  10. Lentiviral Vector Guide

    Type
    Guide
    ...release them into the supernatant. After an initial media change, the supernatant containing the viral ...gene, conferring antibiotic resistance to infected host cells. When these antibiotics are added to the...Gateway cassette containing the ccdB gene, a ccdB-resistant strain is necessary. For more information on cloning...integration site preferences. A major cellular determinant is LEDGF (also known as PSIP1 or p75 ), a lentiviral...predict. In therapeutic applications, like chimeric antigen receptor T cell (CAR-T) therapy, the location of...the genetic information encoded by that genome. Antibiotic resistance is not the only type of selectable... selection marker. Instead of selection by an antibiotic, fluorescence-activated cell sorting (FACS) is...

  11. Chemogenetics Guide

    Type
    Guide
    ...ligands can have high potency, which is potentially important for dosing in animal studies, made them attractive... N ‐oxide (CNO). CNO is a metabolite of the antipsychotic clozapine and seems to be pharmacologically ...however, it has off-target effects. The atypical antipsychotic drug olanzapine has also been shown to be an...domain was engineered to respond specifically to the anti-smoking drug varenicline. This PSAM carries mutations...consider: Chemogenetic activation or inhibition. Do you want to turn ON or turn OFF neurons in your experiment...potentially express the chemogenetic receptor in unwanted cell types. Generating a FLEx switch to control...until a cell or tissue-specific Cre is provided. Want to know more about FLEx vectors? Read our Plasmids...

  12. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... release it into the supernatant. After an initial media change, the supernatant containing the viral ...gene, conferring antibiotic resistance to infected host cells. When these antibiotics are added to the...Gateway cassette containing the ccdB gene, a ccdB-resistant strain is necessary. For more information on cloning...vectors are produced in just a few days. Another variant, Phoenix-gp, contains only gag-pol and enables ...the genetic information encoded by that genome. Antibiotic resistance is not the only type of selectable... selection marker. Instead of selection by an antibiotic, fluorescence-activated cell sorting (FACS) is...immunodeficiency disorders. Delivery of chimeric antigen receptor T cell (CAR-T) therapy is a particularly...

  13. Science Guides

    Type
    Guide
    ... below. Antibodies Chemogenetics CRISPR Optogenetics Pooled Libraries Viral Vectors Antibodies Produced...system, antibodies are proteins capable of highly specific binding to other proteins. Antibody-based applications... often use antibodies conjugated to a signaling molecule, can be used to capture, quantify, and identify...

  14. Promoters

    Type
    Guide
    ... Constitutive Plant Strong promoter from Cauliflower Mosaic Virus Ubi Constitutive Plant High-expression...corresponds to the mRNA transcript produced. The antisense strand is referred to as the template strand or...itself, which helps to explain how many physically distant DNA sequences can affect transcription of a given...

  15. Addgene's eBook Collection

    Type
    Guide
    ... Overlay Select Antibodies 101 New! October 2024 | First Edition About Antibodies 101 October 2024...contains practical introductions and helpful tips for antibody-based applications, from flow to IHC! Close About...

  16. Guide to Using Pooled Libraries

    Type
    Guide
    ...can help you begin to understand what genes are important to a certain phenotype, and allow you to design...Both types of screen employ a selection method relevant to the phenotype being studied. Examples of published...lentiviral CRISPR libraries include: resistance to the anti-cancer drug vemurafenib , resistance to Clostridium...

  17. Educational Resources

    Type
    Guide
    ...your molecular biology research. Topics include: Antibiotic Concentrations for Bacterial Selection What is...

  18. Sequencing Primers

    Type
    Guide
    ...information on molecular biology, plasmids, and recombinant DNA, please consult Addgene's Molecular Biology...
Showing: 1 - 18 of 18 results